Objective To explore the reason of negative HBeAg result detected by ELISA in the specimen with both positive HBV DNA and positive PreS1 in order to provide objective analysis of HBV replication.Methods Senty-six sera with negative HBeAg,but positive PreS1 and HBVDNA were included in this study.(1) HBeAg was detected again by ELISA after the specimen was diluted at a ratio of 1:100.(2) HBeAg/IC was detected by solid ELISA using monoclonal antibody against HBeAg.(3) The gene mutation of Pre C region was detected by oligonucleotide hybridization.Results HBeAg was positive in 5 specimen which were detected in dilution of 1:100 and HBeAg/IC was detectable in 38 specimen.The gene mutation of Pre C region was found in 24 specimen.Conclusion Hook effect of post-zone,formation of HBeAg/IC and gene mutation of Pre C region were the main reasons of negative HBeAg result by ELISA in the specimen with positive HBV DNA.The specimen with positive HBV DNA and negative HBeAg were presented in the patients who were mostly infected by wild type of HBV.Formation of HBeAg/IC result in the undetectable HBeAg in serum by routine ELISA.

Objective To study the chemical constituents in fruits of Catalpa ovata. Methods Isolation and purification were carried out by silica gel, Sephadex LH-20, and active carbon column chromatography etc. Constituents were identified and structurally elucidated by physicochemical properties and spectral analysis. Results Eight compounds were obtained, six of them were determined as catalpol (Ⅰ), catalposide (Ⅱ), ursolic acid (Ⅲ), ?-daucosterol (Ⅳ), nonacosane (Ⅴ), ?-sitosterol (Ⅵ). (Conclusion)(All (~1H-NMR) and (~(13)C-NMR) chemical shifts of the compound) Ⅱ are assigned by UV, IR, ESI-MS, HREI-MS, (~1H-NMR), (~(13)C-NMR), HMQC, and HMBC techniques. Compounds Ⅲ and Ⅴ are isolated from the plant for the first time.

Objective To study the chemical constituents in fruits of Catalpa ovata.Methods Various chromatographic techniques were used to separate and purify the constituents.Structures of the compounds were elucidated by physicochemical properties and spectral analysis (UV, IR, ESI-MS, 1H-NMR , 13 C-NMR , 1H- 1H COSY , HMQC, HMBC, NOESY, and CD).Results A compound was isolated and identified as 2(S)-(3′-hydroxy-5′-methoxy)-benz-3(S)-ethoxycarbonyl-6-trans-ethyl acrylate-8-methoxy-benzofuran.Conclusion This compound is a novel compound named as catalpafurxin.

Objective: To evaluate the properties of PLA-PEG as a biodegradable scaffold material combined with rhBMP-2 for bone construction.Methods:PLA-PEG/rhBMP-2 multipore compound was prepared,then compression intensity and compression module were tested.The mandibular defect model was made in 20 rabbits,then PLA-PEG/rhBMP-2 was implanted into the defects on one side,and PLA-PEG without rhBMP-2 was implanted into the defect on another side as the control.The bone specimens were retrieved to make density analysis with X-ray photogram following sacrifice of the rabbits 2,4,8 and 16 weeks respectively after operation,then the specimens were decalcified to make histopathological observation by means of HE stain.Results:Compression intensity(MPa) of the PLA-PEG/(rhBMP)-2 compound was 30.05?3.12,and compression module(MPa) was 371.67?12.37.At 2,4,8 and 16 weeks,more new bone was observed,and higher intensity(P

Urothelial carcinoma associated 1 (UCA1) is a kind of long non-coding RNA (lncRNA),which has no capacities for coding proteins.UCA1 over-expresses in diverse tumors,and plays a pivotal role in initiation and progression of tumors.Researches indicate that UCA1 may function as an oncogene in gastrointestinal tumors,such as gastric cancer,colorectal cancer and hepatocellular cancer,and participate in regulating cell proliferation,metastasis and chemo-resistance.

Rhetsinine has been isolated from the fruits of Evodia rutaecarpa ( Juss. ) Benth. ~1H NMR and ~(13)C NMR assignments reported previously for rhetsinine were revised on the basis of UV, IR, ESI-MS,~1H NMR, ~(13)C NMR and X-ray crystallographic analysis.

Objective To analyze the syphilis epidemic characteristics and trends of Yongchuan District during 2010? 2015 to provide a basis for formulating the prevention and control strategy of syphilis.Methods The data of syphilis cases reported in Yongchuan District of Chongqing City during 2010-2015 were arranged,checked anal statisticallyanalyzed.Results The incidence rate of syphilis showed a reverse U type change trend from rising to declining,latent syphilis accounted for the highest proportion,congenital syphilis cases were decreased year by year.The analysis results showed that the constituent ratio of latent syphilis and congenital syphilis had statistical difference between 2010-2013 and 2014-2015(P＜0.05);syphilis high prevalent area was concentrated at the city and town area,while prevalence rate was slower in rural area.Most of syphilis patients were concentrated in the age group of 25-45 years old.There was no difference between women and men;syphilis cases generally had lower educational level,majority concentrated at the population of junior high school or below;the occupations in the syphilis patients were concentrated at the entertainment personnel,followed by migrant workers.Conclusion The incidence rate of syphilis is changed from rising to smoothly and steady decreased.It is necessary to adopt the pertinent prevention and control measures for the high-risk population.

Objective To investigate the relationship between serum vitamin A deficiency (vitam in A deficiency, VAD) and infectious diseases in children.MethodsAdopt the method of random sampling, sample of Cixi City prevention center with a total of 5000 children under the age of 6, according to the age groups were divided into 6 groups, the detection of vitamin A in children refers to the blood, separation of serum collected in dark conditions, with strict quality control, detection of serum vitamin A by fluorescence method (vitamin A the concentration of VA.).ResultsIn the two weeks, the incidence of VA in children with fever and acute respiratory infection (ARI) increased.ConclusionOur children's serum VA content is low, the lack of the high rate of children regardless of individuals or groups are prone to acute infection, VAD is the cause of children prone to acute infectious diseases is one of the important reasons.To strengthen health education, improve parents' feeding knowledge, proper oral Cod Liver Oil drops and reasonable feeding, is conducive to the healthy growth of children.