OBJECTIVE:To establish a RP-HPLC method for the determination of Esculetine in Dinglian tablets.METHODS:The chromatographic column was Hypersil ODS C18(200mm?4.6mm,5?m)at a temperature of 25℃;the mobile phase was methyl-water-glacial acetic acid(40∶60∶0.4)at a flow velocity of 1.0mL?min-1,and the detection wavelength was set at 353nm.RESULTS:The linear concentration range of Esculetine was within 0.045～0.360?g(r=0.999 5).The average recovery rate was 97.72%(RSD=1.87%,n=5).CONCLUSION:This procedure is simple,accurate and reliable,and it can be used for the quality control of Dinglian tablets.

Humans ; Transcriptome/genetics* ; East Asian People ; Genetic Predisposition to Disease ; Genome-Wide Association Study ; Pulmonary Disease, Chronic Obstructive/genetics* ; Polymorphism, Single Nucleotide/genetics*