1.Expression of Proliferating Cell Nuclear Antigen and B-cell Lymphoma/Leukemia-2 Gene in Cholesteatoma and Its Clinical Implication
Hongguang PAN ; Yonghua CUI ; Qixue GAO
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2001;30(2):163-164
The expression of proliferating cell nuclear antigen (PCNA) and B-cell lymphoma/leukemia-2 gene (Bcl-2) was detected in 25 cases of cholesteatoma tissues and 10 cases of normal skin by using immunohistochemical technique. The results showed that the PCNA index of cholesteatoma tissues was higher than that of normal skin, but Bcl-2 was not expressed in cholesteatoma tissues and normal skin. The PCNA index was correlated with the aggressiveness of cholesteatoma. It was suggested that PCNA could be considered to be a prognostic marker and an indicator of aggressive behavior in cholesteatoma. Although the cholesteatoma had a very high proliferating character, it wasn't a malignant tumor.
2.DNA character of the cholesteatoma DNA character of the cholesteatoma
Yonghua CUI ; Hongguang PAN ; Qixue GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2001;(2):62-63
Objective:To compare the DNA character with in cholesteatoma tissues with that in normal skin.Method:DNA content within 25 cholesteatoma tissues and 10 normal postauricular skin was measured with flow cytometry.Result:The content of cholesteatoma tissues and postauricular skin specimens are a normal euploid.The cell number during S phase of cholesteatoma(10.9±2.32)% was higher than that in normal skin(7.31±2.82)%.Conclusion:Although the cholesteatoma has a very high proliferating character,the result suggest it isn′t a malignant tumor.
3.A case of malignant melanoma on the left side of the maxillary sinus.
Wanqing NIAN ; Heng WANG ; Qixue GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(4):279-280
The clinical manifestations of the disease lacked specificity,and the clinical symptoms were nasal obstruction, any blood in snively and slightly swelling pain on face. The neoplasm with unsmooth surface looked taupe and maroon, and was brittle and easy blooding. Paranasal sinus CT:crumbly mass lesion within the shadow filled in the left side of the maxillary sinus,and the sinus stopped up,sinus wall bone was absorpt and thinned, front and rear wall and inner wall bone were boundedness broken off, knuckle partly to the nasal cavity. Direct reinforcement MRI:in addition to the result of paranasal sinus CT,enhance examination found obviously asymmetrical intensify of the focus. The pathological diagnosis of postoperation was malignant melanoma on the left side of the maxillary sinus.
Humans
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Male
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Maxillary Sinus Neoplasms
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Melanoma
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Middle Aged
4.Inhibitory Effects of Glucocorticoids on the Tenascin C Expression in Nasal Polyp
Heng WANG ; Zheng LIU ; Xiang LU ; Xuejun YOU ; Qixue GAO ; Yonghua CUI
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2009;38(6):756-759
Objective To investigate the effect of glucocortieoids on tenasein C(TNC) expression in nasal polyp tissues and airway epithelia,and explore the possible mechanism of glucocorticoids inhibiting remodeling of nasal polyp tissue.Methods The enzyme linked immunosorbent assay(ELISA) was used to detect the protein levels of tenascin C and transforming growth factor-β1(TGF-β1) in nasal polyp tissues from intranasal glucocorticoids (Budesonide,BUD) treated group and untreated group.The cell culture,real-time RT-PCR and in situ ELISA were employed to investigate the regulatory effects of budesonide on the TNC mRNA and protein expression in BEAS-2B immortalized human bronchial epithelial cells.Results The protein levels of TNC and TGF-β1 were decreased in nasal polyp tissues from intranasal BUD-treated group as compared with untreated group(P<0.01).There was a significantly positive correlation between TNC and TGF-β1 protein levels in nasal polyp tissues (r =0.68,P<0.01).After preincubation with BUD,TNC mRNA and protein expression induced by TGF-β1 in BEAS-2B cells was significantly decreased(P<0.01).Conclusion Glucocorticoids might participate in the regulation of tissue remodeling in nasal polyp by inhibiting the TGF-β1 expression in nasal polyp tissue and suppressing the induction of TGF-β1 to up-regulatie the TNC expression in airway epithelia.
6.Computed tomographic images analysis of frontal recess anatomy based on three-dimension reconstruction.
Peng WANG ; Xiaodong HAN ; Gui YANG ; Yanhong ZHANG ; Jianping LAN ; Hailiang ZHAO ; Shuqi QIU ; Hongtao ZHEN ; Qixue GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(17):1305-1310
OBJECTIVE:
To observe the CT three-dimensional imaging features of the frontal recess region with advanced three-dimensional reconstruction, and develop the real image of the important anatomical structures around the region to conduct surgery.
METHOD:
Thirty patients were undergone spiral CT by 16 line high speed spiral CT, and multiplanar reconstruction images using standard three-dimensional reconstruction protocol on a computer workstation. The structure of the frontal recess, the agger nasi cell and adhere style of the uncinate process were observed. The parameter of the important anatomic structure of frontal recess was measured precisely.
RESULT:
After the reconstruction, we get the three-dimensional model very close to the true state of the nasal cavity-sinuses cell, in which parts of the frontal recess can clearly identify the agger nasi cell, frontal cell and other important structures. In these patients, the height, width and depth of the agger nasi and frontal sinus were (9.45 ± 3.60)mm, (8.08 ± 3.37)mm, (26.98 ± 6.82)mm and (26.86 ± 9.45)mm, respectively.
CONCLUSION
This study tried to develop the standardized techniques and measurements from three-dimensional reconstructed images of the frontal sinus and to ascertain the usefulness of the frontal sinus in identification of patients. The project results in better preoperative patient counselling and in predicting postoperative improvement in clinical status.
Ethmoid Sinus
;
diagnostic imaging
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Frontal Sinus
;
diagnostic imaging
;
Humans
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Image Processing, Computer-Assisted
;
methods
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Imaging, Three-Dimensional
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Nasal Cavity
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Paranasal Sinuses
;
diagnostic imaging
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Tomography, Spiral Computed
;
methods
7.Three dimensional reconstruction study of multi-slice helical CT imaging on optic canal area.
Zhihai LI ; Qixue GAO ; Zhiyi CAL ; Baohong TAO ; Jingyao LÜ
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2007;21(1):7-9
OBJECTIVE:
To investigate the accuracy and clinical application of multi-slice spiral CT imaging on optic canal area.
METHOD:
Forty intact specimens of human corpse head were tested with three dimensional reconstruction of multi-slice spiral CT imaging. The results of radiology and anatomy were compared on adjacent structures of optic canal interior wall and its developmental relationship with sphenoid sinus.
RESULT:
(1) The high spatial resolution of integrity could be obtained with dimensional processing technique. The anatomical structure of optic canal and its surrounding area could be demonstrated clearly. (2) With the technique of virtual endoscopy, the interior reconstruction of optic canal could demonstrate the inner topography of optic canal, the reconstruction image of which was identical to that of the control subjects. (3) There was no significant difference between the data of multi-slice spiral CT imaging and that of anatomy (P > 0.05).
CONCLUSION
Associated application of dimensional processing technique and virtual endoscopy, not only can the subtle structures of optic canal be demonstrated and measured, but also the detailed information on spatial relationships of optic canal area can be provided for operation.
Adult
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Asian Continental Ancestry Group
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Female
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Humans
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Imaging, Three-Dimensional
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Male
;
Optic Nerve
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anatomy & histology
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diagnostic imaging
;
Tomography, Spiral Computed
8.Prevalence of sensitization to allergens in patients with allergic rhinitis and respiratory disease in Qingshan of Wuhan.
Peng WANG ; Wei LI ; Ping ZHAO ; Youyun LOU ; Qixue GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2008;22(14):634-636
OBJECTIVE:
The purpose of this study was to determine which allergens were prevalent in patients with allergic rhinitis and respiratory disease in Qingshan Wuhan.
METHOD:
A total of 700 cases, which were diagnosed as allergic rhinitis and respiratory disease by history and clinical presentation, underwent ELISA test with 7 standardized allergens, and the most important sensitizing allergens were assessed. The samples were stratified on two age groups: AR and respiratory disease group and the positive rate of two main allergens in two groups were contrast analyzed.
RESULT:
For patients with allergic rhinitis, the positive rate of the top seven aeroallergen were dermatophagoides (47%), herbs (19.7%), tree (18%), animal dander (8.9%), house dust (6.5%), mold (4.9%) and wormwood (3%). There was no significant difference in higher sensitivity to dust mite and ragweed between allergic rhinitis and respiratory disease group (P > 0.05).
CONCLUSION
Dust mite, Tree and Herbs are the most important three aeroallergens in Qingshan region of Wuhan.
Adult
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Aged
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Aged, 80 and over
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Allergens
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analysis
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Antigens, Dermatophagoides
;
analysis
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China
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epidemiology
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Dust
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analysis
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Female
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Humans
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Immunoglobulin E
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analysis
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Male
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Middle Aged
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Pollen
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Prevalence
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Rhinitis
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epidemiology
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Young Adult
9.3D computed tomographic analysis of frontal recess region.
Peng WANG ; Yanhong ZHANG ; Xiaodong HAN ; Gui YANG ; Jianping LAN ; Hailiang ZHAO ; Shuqi QIU ; Hongtao ZHEN ; Qixue GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(5):311-317
OBJECTIVE:
The purpose of the study was to observe the three-dimensional (3D) CT imaging features of the frontal recess region with 3D reconstruction, and obtain the real image of the important anatomical structures of the region to conduct surgery.
METHOD:
Five patients were undergone spiral CT by 16 line high speed spiral CT, and multiplanar reconstruction images using standard 3D reconstruction protocol on a computer workstation. The structure of the frontal recess, the agger nasi cell and adhere style of the uncinate process were observed. The parameter of the important anatomic structure of frontal recess was measured precisely.
RESULT:
After the reconstruction, we get the 3D model very close to the true state of the nasal cavity--sinuses cell, in which parts of the frontal recess can clearly identify the agger nasi cell, frontal cell and other important structures. In this patient, the height, width and depth of the agger nasi and frontal sinus were 12.3 mm, 12.1 mm, 38.5 mm, respectively.
CONCLUSION
This study tried to develop the standardized techniques and measurements from 3D reconstructed images of the frontal sinus and to ascertain the usefulness of the frontal sinus in identification of patients. The results in better preoperative patient counselling and in predicting postoperative improvement in clinical status.
Adolescent
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Adult
;
Aged
;
Female
;
Frontal Sinus
;
diagnostic imaging
;
Humans
;
Imaging, Three-Dimensional
;
Male
;
Middle Aged
;
Tomography, Spiral Computed
;
Young Adult
10.Computed tomographic analysis of frontal recess region.
Peng WANG ; Xunhua XU ; Ping ZHAO ; Hongtao ZHEN ; Qixue GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;23(5):214-218
OBJECTIVE:
The purpose of the study was to observe the CT imaging features of the frontal recess regional.
METHOD:
Eighty-two patients were undergone spiral computed tomography (CT). Then multiplanar reconstruction images were made using standard triplanar reconstruction protocol on a computer workstation.
RESULT:
The prevalence of agger nasi cell was 87.8% (144/164). One hundred and forty-five (89%, 145/164) uncinate processes had one superior attachment for each uncinate process. The others had two superior attachments for each uncinate process. The uncinate process' single superior attachment of into the surrounding structures was identified to have the following distribution: 90/164 (54.9%): to the lamina papyracea, 50/164 (30.5%) to the middle turbinate, and 5/164 (3.0%) to the skull base. Of all the frontal cells were identified in 144 (87.8%) sides of frontal recesses, the prevalence of type I II, type III IV, were 30.5% and 9.8% respectively.
CONCLUSION
T Agger nasi cell and uncinate process play an important role in endoscopic frontal sinus surgery.
Adolescent
;
Adult
;
Aged
;
Child
;
Female
;
Frontal Lobe
;
diagnostic imaging
;
Frontal Sinus
;
diagnostic imaging
;
Humans
;
Image Processing, Computer-Assisted
;
Male
;
Middle Aged
;
Nasal Cavity
;
diagnostic imaging
;
Tomography, X-Ray Computed
;
Turbinates
;
diagnostic imaging
;
Young Adult