The etiology in Basic of TCM was six exopathogens (wind, cold, summer heat, wet, dryness, and fire),seven emotions, blood stasis, phlegm retention, improper diet, exhaustion, infectious damp heat, trauma and bitten by animal and insect. Through years of observation, we found that some diseases could not be explained by such etiologies and thus put forward some new etiologies, including pollution poison gas, drugs, chemicals and radiation. These new etiologies had some significance in improving TCM theories and guiding clinical practice.

ObjectTo establish HPLC fingerprint for the identification and evaluation of Caulis Spatholobi. Methods HPLC system was used to obtain the chromatograms of Caulis Spatholobi and the clustering analysis was applied for data analysis. Results There are 11 peaks in total, among them five are common, the relative retention time is 0.046, 0.664, 1.000, 1.198, 1.383, the relative peak area is 0.819,0.221,1.000,0.331,0.458. There was obvious difference among chromatographic fingerprints of Caulis Spatholobi and its easily-confused species. The 11 measured peaks could be used as the fingerprint features. Conclusion Chromatographic fingerprint of HPLC can be used for identifying the Caulis Spatholobi and its easily-confused species.

Nano-hydroxyapatite biomimetic bone materials have become a hotspot in the field of tissue engineering research due to the similarity of the structure and composition to natural bone. This article describes a variety of preparation of nano-hydroxyapatite and synthesis of nano-hydroxyapatite composite, as well as the properties of nano-hydroxyapatite composite materials. Through surface-modification nanohydroxyapatite composite materials will have a potential application foreground including bone defects repair,drug carrier for cancer treatment. In this paper, research progress of nano-hydroxyapatite biomimetic bone materials in recent years were reviewed.

Xenogeneic/Allogeneic bone materials are among the hot research interests of biological scaffolds.These materials are ideal materials for repairing bone defects because they have the structure and morphology of the natural bones,are able to be degraded and absorbed in the body,and are less immunogenic through appropriato treatment.Through further modification with biological molecules such as growth factors,they could provide a better microenvironment for the bone cells to grow into and fully differentiate.This article summarizes the factors affecting the immunogenicity of xenogeneic/allogeneic bone and the methods of removing immunogenicity.Advances in sintered bone materials,defatted bone materials,deproteinated bone materials,decalcifying bone materials,and composite materials are reviewed in greater details.

Objective To evaluate the effects of cerebral ischemia/reperfusion or/and icariin on expression of cytochrome C oxidase subunitⅡ(COⅡ) mRNA in mice. Methods The mouse model of transient cerebral ischemia/reperfusion was made by bilateral occlusion of common carotid arteries and ischemic hypotension/reperfusion. The mice were divided into several groups at random: normal control group, model group and icariin preventive treatment group (100 mg/kg). The changes in expression levels of COⅡ mRNA in mice cerebral tissue were detected by RT-PCR at different time points. Results The levels of CO Ⅱ mRNA in model group had no significant changes at 1 h and 24 h after cerebral ischemia/reperfusion as compared with that in control group, but decreased remarkably at 3 h (P

Objective To investigate the radiographic features and surgical approach of severe fixed cervical kyphosis.Methods Seventeen cases of severe fixed cervical kyphosis from January 2007 to January 2012 were studied retrospectively.There were 8 males and 9 females,with an average age of 49.7 years.The etiologies were 3 cases of infection,3 cases of degeneration,3 cases of neurofibromatosis,3 cases of prior laminectomy,2 cases of idiopathic,2 cases of trauma and 1 case of neuromuscular disease.The average length of kyphosis was 4.3 ± 1.2 segments.All patients complained of severe neck pain (visual analogue scale,VAS,7.6±1.5) or progressive cervical kyphosis.There were 4 cases with myelopathy,2 with radiculopathy,3 with difficulty of forward gaze and 1 with difficulty of swallowing.All patients were underwent dynamic flexion-extension radiographs and traction views.CT scans were carried out to identify the sites of fixed kyphosis.Continuous traction was performed after general anesthesia,and the surgical approach was decided according to spinal cord compression,length of kyphosis and the cause of fixed kyphosis.Surgical outcomes were assessed in terms of correction of Cobb angle and Odom criteria.Results All patients were followed-up for 2 to 5 years.According to the CT scans,the sites of fixed kyphosis were identified:anterior bony ankylosis in 7 cases,posterior in 6 cases and both anterior and posterior in 4 cases.The surgical approach were as following:anterior only in 4 cases,posterior only in 2 cases,anterior-posterior in 5 cases,posterior-anterior in 3 cases,anterior-posterior-anterior in 1 case and posterior-anterior-posterior in 2 cases.The Cobb angle was corrected from 49.3°± 14.6° preoperation to 2.1 °±6.8° at the latest follow-up,with an average correction of 47.2°.According to Odom criteria,there were 7 excellent outcome,8 good,2 fair and none poor outcome.Revision surgery was performed in 1 case due to proximal junctional kyphosis.At the latest follow-up,bony fusion was found in all patients.Conclusion CT scan is helpful in identifying the cause of fixed kyphosis.The surgical approach of fixed cervical kyphosis is decided by spinal cord compression,length of kyphosis and cause of fixed kyphosis.Anterior approach is suitable for anterior ankylosis,posterior approach for posterior ankylosis,combined approach for both anterior and posterior ankylosis.

Objective To evaluate the value of upright standing myelography in the diagnosis of lumbar disc herniation (LDH). Methods One hundred and ninety six patients with LDH diagnosed by CT or MRI were examined by upright standing myelography before surgery. Results The most diagnosis of myelography were accorded with CT or MRI, but 7 patients of myelography with LDH at L4-5 showed compression of nerve roots, while the images of these on CT or MRI only showed disc bulge or protrusion without nerve root compression. Conclusions Myelography in the upright standing position is perhaps superior to CT or MRI for the diagnosis of LDH, especially LDH at L4-5 with nerve root compression.

Objective To investigate the correlation between MR imaging and pathological features of nucleus pulposus degeneration in the lumbar disc, and to provide a reliable evidence to evaluate the nucleus pulposus degeneration by MR imaging relative signal intensity. Methods From January 2001 to December 2001, 91 patients who underwent operative treatment for lumbar disc diseases were recruited for the study, while 4 lumbar fracture patients with no previous low back pain and 2 fresh brain-injury cadavers were recruited for contrast study. Before operation, the relative signal intensity was measured in MRI T2WI images. All 117 specimens were dyed with HE and Alcian blue CEC. The water content was also detected. The correlation between MRI relative signal intensity and chondroitin sulfate index, water content was analyzed with SPSS 10.0 software, the correlation between chondroitin sulfate index and water content was analyzed as well. Results There was significant difference between the above mentioned two groups when MRI relative signal intensity, chondroitin sulfate index and water content was compared. Whereas there was no significant statistical difference when keratin sulfate index was compared between the two groups. MRI relative signal intensity positively correlated with water content and chondroitin sulfate index, and the chondroitin sulfate index positively correlated with water content. Conclusion The MRI relative signal intensity reflects not only the water content, but also the pathological characteristics of nucleus pulposus degeneration. The quantitative classification of relative signal intensity in MRI is a method to identify the grades of nucleus pulposus degeneration.

[Objective]To investigate the treating effect of bicondylar tibial plateau fractures with lateral supporting plate and compression screw system.[Method]Forty-six cases of bicondylar tibial plateau fractures were treated with internal fixation from the year of 2000 to 2004 in our hospital.All of them were treated with open reduction and internal fixation by lateral supporting plata and compression screw system.Orthopaedic evaluation at latest follow-up included a knee functional outcome and radiographic examination.[Result]Forty-six cases were followed up for 12 to 18 months(averaged,14.4 months).All fractures healed.According to Rasmussen scale,84.8% excellent and good results were obtained.[Conclusion]Most of all bicondylar tibial plateau fractures were stabilized by applying lateral supporting plate and compression screw system.This system help reduce complication rates resulting from open reduction and internal fixation.

Objective To investigate the safe directions of screw trajectory in atlas via posterior arch and lateral mass and its clinical significance. Methods Lateral radiographs and CT axial scans of atlases were performed in 30 cases with normal morphology of atlases and axes. The minimal height of posterior arch, the maximum inclination of screw projection relative to sagittal plane, and the maximum medial angle of screw projection relative to axial plane were evaluated radiologically. According to the safe directions obtained radiologically 21 cases of atlantoaxial instability were treated with screw fixation atlas via posterior arch and lateral mass. During operation the influence of screws on surrounding structures was investigated and postoperative neural symptoms were documented also. Preoperative and postoperative radiographs and CT scans of 13 patients were available and some related parameters were measured to evaluate the safety of the screw placements. Results 1) The maximum angle of screw projection to sagittal plane is about 10? cephalad to 6? caudal, with the tendency of increasing maximum angle as the minimal height of posterior arch increases. 2) When the entry point on the posterior arch was switched laterally, the medial angle of screw projection should be adjusted from 0? to 30?, correspondingly. 3) The actual directions of screw trajectory might differ from preoperative ones, but all were in the estimation range. 4) All screws were placed successfully, and the postoperative radiographs and CT scans shows no neural or vascular complications relative to atlantal screws placed in traditional way. Conclusion There is a safe range to insert atlas screw via posterior arch and lateral mass both in sagittal and axial plane.