ObjectiveTo selected the safe dosage of intrathecal injection of sufentanil for preemptive analgesia in lower limb orthopaedic surgery.MethodsSelected 96 patients who ASA classification Ⅰ - Ⅱ grades undergoing elective lower limb orthopaedic surgery with subarachnoid epidural block down,they were divided into six groups by random number table and double blind method,each group of 16 cases.Group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ received 0.05,0.10,0.15,0.20,0.25,0 μ g/kg of intrathecal sufentanil and 2.5 ml of 0.5％ levobupivacaine.Observed and recorded the duration of block level of pain to T10,visual analog score (VAS)postoperative at 2,4,6,8,12,24,48 h,and side effects.ResultsThe duration of block level of pain to T10 of group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ was (245.6 ± 34.2),(259.3 ± 36.7),(268.9 ± 37.8),(286.6 ± 38.8),(287.7 ± 39.1 ),(228.6 ± 31.4) min.There was no significant difference between group Ⅳ and group Ⅴ (P ＞ 0.05 ),the differences of other groups were statistically significant (P ＜ 0.05 ).Compared with group Ⅴ,VAS of group Ⅲ and group Ⅳ at 6,8,12,24,48 h after operation was not statistically significant (P ＞ 0.05),and they were lower than the other three groups(P ＜ 0.05 ).There were no respiratory depression occurred after operation in group Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅵ,4 cases occurred in group Ⅴ.ConclusionThe best of effective dose of intrathecal injection of sufentanil for preemptive analgesia in lower limb orthopaedic surgery is 0.20 μ g/kg.

This study was an attempt to excise or transplant optic vesicles, and excise or transplant presumptive lens ectoderms, by means of microscopic manipulations. Its purpose was to explore the dependence of the development of lens on the eye cup and the ability of induction of the eye cup to develop lens in liana nigromaculata, R. limnocharis, and Kaloula barealis, which are frequently used in experimental embryology in China. The results show that their lenses cannot develop without eye vesicles. This indicates that they belong to the pattern of dependent differentiation. The results also indicate that the situation of forming lenses from ectoderms under induction of eye cup is different in three kinds of frogs: the lenses can be frequently formed from ectoderm of both Lead and abdomen in Rana nigromaculata; but the frequency of lens formation from ectoderm of abdomen is rather low in R. limrocharis; and only the ectoderm of head is positive in Kaloula barealis. The interrelation in size between the eye cup and the lens was recorded. The formation of a secondary eye cup from brain wall under condition of the contact with the presumptive lens ectoderm was reported too. In addition, it was found that the temperature might have influence on eye differentiation.

The mesenchymal cells were carefully scraped off from the optic vesicles or eye cups at 15 to 24 stages in Rana limnocharis by a hair loop (those specimens from 21 to 24 stages were treated with trypsin in order to separate the mesenchymal cells easier) and then the optic vesicles or eye cups were transplanted into the coelom of tadpoles for 1 to 2 weeks. The transplantation of the eye cups Without scraping mesenchymal cells off at 21 to 24 stages were used as control. The results are as follows: 1.The pigment epithelium of eye, which was transplanted at 15 to 20 stages, (the pigment epithelium have not differentiated at those stages) can not fully develop or only a tiny piece of pigment epithelium layer occurs. 2.The pigment epithelium of eye, which was transplanted at 21 to 24 stages, (the pigment epithelium have differentiated at those stages) can develop, but separates itself from the normal position and hangs outside the eye or curls inside the cavity of eye in a vesicular form. 3.In the intact mesenchymal cells, the pigment epithelium of eye, which was transplanted at 21 to 24 stages, can develop well and remain in normal position. These results suggest that the mesenchymal cells might have an important effect on the development and morphology of eye pigment epithelium before and after its differentiation. Before differentiation of pigment epithelium, the existence of mesenchymal cells is a necessary condition for its normal differentiation; after its differentiation, the mesenchymal cells might play a mechanical role to keep the pigment epithelium layer in normal position.

It is well-known that the presumptive significance of every part of the optic vesicle has already been decided. The centrifugal part (i. e. the far end wall) of the optic vesicle is called presumptive retina and its centripetal part, (i. e. the near end wall) the presumptive pigment epithelium. In the present study, we caused the latter to come into contact with different tissues and organs by transplanting and excising for the purpose of observing whether it would be able to alter normal course and form retina. The experimental material was the embryos of frogs (Rana nigromaculara, R. japonica and Bufo bufo gargarizans). The experiment was divided into two groups: (1) brought presumptive pigment epithelium into contact with presumptive lens ectoderm, head ectoderm and abdomen ectoderm; (2) brought it into contact with other organs and tissues, including otic vesicle, pericardial membrane, nephridial tubule, pharyngeal wall, muscle, coelomic membrane, hepar, and cartilage.The results indicate that the contact with three kinds of ectoderm can alter normal course of development of presumptive pigment epithelium, transform it into retina and further produce secondary eye cup. As regard to its frequency of transformation into retina, the highest is in the case contacted with presumptive lens ectoderm; in the case contacted with head ectoderm come next and the lowest in the case contacted with abdomen ectoderm. The results also show that the above-mentioned organs and tissues can cause similar reactions, but the frequency of transformation is lower than that caused by abdomen ectoderm, and there are very few secondary eye cups which appear only under the conditions of contacting with otic vesicle, coelomic membrane and cartilage. This suggests that the action of the contact which brings presumptive pigment epithelium to transform into secondary retina might be an induction and material of induction widely distributed in the embryonic body.Two other reactions, moreover, were also found, i. e. the presumptive pigment epithelium was transformed into brain tissue and merged after the former came into contact with the latter, and the transplanted eye and the host eye fused in part or fully merged into a large eye when they came into contact.Above-mentioned phenomena further prove that the optic vesicle has a certain plasticity.

This paper analyzed the problems existing with the usage of assistive ambulatory devices (AADs) among older adults in America and proposed solutions to improve these problems. The effects of AADs － walker, rollator, and cane － on gait and posture of older adults were assessed. Also, the relationships between AAD usage, fall occurrence, and why older adults continue to fall despite use of AADs were analyzed. It was suggested that in order to prevent from falls among older adults in the community routine assessment and training in correct AAD use should be performed.

OBJECTIVE:To study protective effects of glutamine (Gln) on cardiac muscle cell in septic model rats. METH-ODS:Rats were randomly divided into sham operation group (normal saline),model group (normal saline) and Gln low-dose, medium-dose and high-dose groups(0.5,0.75,1.0 g/kg)with 10 rats in each group. In these groups,septic rat model was induced by cecal ligation and puncture except sham operation group received sham operation. They were given relevant medicine intrave-nously 10 min after operation,and the characteristics and apoptosis of cardiac muscle cell were observed 12 h after operation. The serum contents of CK,LDH and TnⅠ,and the expression of Bcl-2 and p53 mRNA were all detected. RESULTS:Compared with sham operation group,myocardial necrosis of model group was found,and the serum content of CK,LDH and TnⅠ and apoptotic index increased,and mRNA expression of Bcl-2 in cardiac muscle cell decreased while that of p53 increased,with statistical signifi-cance(P<0.05). Compared with model group,myocardial injury relieved significantly in Gln high-dose and medium-dose groups, and serum contents of CK,LDH and TnⅠ and apoptotic index decreased;mRNA expression of Bcl-2 increased in cardiac muscle cell while that of p53 decreased,with statistical significance (P<0.05). CONCLUSIONS:Gln can improve myocardial injury of septic model rats significantly,by a possible mechanism of down-regulating the expression of p53 gene and up-regulating the ex-pression of Bcl-2 gene.

On the background of internationalization for higher education,we have reformed the educational model for bachelor students majoring in public health laboratory science and quarantine.The exploration and pilot study are focused on educational principles,curriculum planning,teaching patterns,experiments and practice,and teachem training.The four teaching strategies have been firstly recommended as follows:laying the strongest foundation of chemistry,expanding quarantine-related curricula for increasing quarantine capability,making full use of strength of clinical laboratory medicine,emphasizing English application.The curricula structure is refined and more social supporting resources have been got to make globalization to cultivate more health inspection and quarantine talents with multidisciplinary knowledge,abilities and strong adaptability.

Three types of microscopic operations were used in this study: 1. excision of the optic vesicle alone; 2. after excising the optic vesicle, a piece of forebrain tissue taken from another donor was inserted in between presumptive lens ectoderm and forebrain wall; 3. cut down the optic vesicle and portion of forebrain tissue and replaced them back in situ by turning over 180?. The operations were carried out on Rana nigromaculata at 15 and 16 stages, in order to bring the presumptive lens ectoderm to come into contact directly with the forebrain tissue for the purpose to analyse the possibility of eye cup formation from the brain wall. The results indicate that the forebrain wall which came into contact with the presumptive lens ectoderm could be induced and differentiated into a secondary eye cup or retina. A total of 81 cases of secondary eye cups among 282 operations (28.8%) were observed. The mechanism for the induction and its significance were discussed.

Aim To investigate the effect of catalpol from Radix Rehmanniae on M_2 receptor density in CHO cells transfected with gene of M_2 receptors(CHO m2).Methods Cultured CHOm2 cells were randomly divided into 4 groups:three concentrations of catalpol :10-6、10-5、10-4 mol?L-1,and saline control.After addition of catalpol and saline for 72 h,M_2 Receptor density was measured by single point 3H-NMS binding assay,the content of protein was measured with Lowry's method.Competitive binding assay using the binding system of 3H-NMS was performed to address the question about whether catalpol could occupy the M receptor binding site.Addition of catapol to brain homogenate and measuring the enzyme activity with the Ellman's colorimetric method were performed to address the question about whether catalpol could in-hibit acetylcholinesterase activity.Results Catalpol can elevate the M_2 receptor density in CHO m2 cells significantly at the doses of 10-5、10-4 mol?L-1(P

Aim To observe the effect of ZDY102, a C25 stereo-isomer of ZMS, the active component of Zhimu, on brain M receptor density of dementia model animals and the correlation with its effect on learning/memory ability. Methods The rats were randomly divided into five groups: control group, model group, model given orally for 2 months with 3.6 mg?kg -1?d -1 of ZDY102 treatment, model treated with 9.0 mg?kg -1?d -1 of ZDY102, and model treated with 18.0 mg?kg -1?d -1 of ZDY102. Dementia model was produced by single unilateral injection of 4 ?l of normal saline containing 4 ?g of ?-amyloid (25～35) and 1 ?g of ibotenic acid into right basal ganglion region with the aid of a stereotaxic equipment. The brain muscarinic receptor density was analyzed with single-site binding assay using 3H-quinuclidinyl benzilae(QNB). The learning/memory ability was measured by Y-maze performance. Results Two months after model production, the learning and memory ability as well as the density of muscarinic receptor in brain were significantly decreased in model rats compared with those in control rats. Parallel models treated with daily oral administration of ZDY102 for two months improved in learning and memory ability and their muscarinic receptor density was significantly increased when compared with model rats. The correlation coefficient between total M receptor densities and the learning/memory ability was significant when examined with linear regression. Conclusion ZDY102 can significantly improve the learning and memory ability and increase the brain muscarinic receptor density of the model. Since brain muscarinic receptors are closely correlated to learning and memory, up-regulation of M receptor density might play a very important role in the therapeutic effect of ZDY102.