1.Antimicrobial resistance and SCCmec genotype of methicillin-resistant Staphylococcus aureus
International Journal of Laboratory Medicine 2014;(18):2512-2514
Objective To investigate antibiotic resistance and Staphylococcal cassette chromosome mec (SCC-mec) genotype of methicillin-resistant Staphylococcus aureus (MRSA)in Dalian Area .Methods Antimicrobial susceptibility of MRSA was detected by Kirby-Bauer method and SCCmec genotype by multiple PCR method .Results 38 strains of MRSA were all multidrug-resistant strains ,but susceptible to vancomycin and chloramphenicol .The most common SCCmec genotype was type Ⅱ (86 .84% ) ,type Ⅲwas in the second place(10 .53% ) ,and unidentified type accounted for 2 .6% .Conclusion MRSA strains in Dalian area are charac-terized by multidrug resistance ,type Ⅱ is the most common SCCmec genotype .
2.Role and mechanism of thrombin in white matter injury after intracerebral hemorrhage
International Journal of Cerebrovascular Diseases 2017;25(5):449-453
Intracerebral hemorrhage accounts for about 10%-30% of all stroke types.It is characterized by rapid onset, rapid progress, varied clinical symptoms, high morbidity and mortality, and poor prognosis.After the intracerebral hemorrhage, various inflammatory mediators result in white matter lesions and cognitive impairment.Thrombin acting on thrombin receptors at low concentration induces neuroprotection and at high concentration causes brain injury.This article reviews the role and mechanism of thrombin in white matter injury after intracerebral hemorrhage.
3.Detection of Escherichia coli O157:H7 by multiplex PCR-DHPLC
Junyi XU ; Jijuan CAO ; Qiuyue ZHENG ; Qiuyan WANG ; Shuyan LIU ; Dan JIANG
Chinese Journal of Microbiology and Immunology 2009;29(4):366-369
Objective To develop the PCR-denaturing high performance liquid chromatography (DHPLC) for detection of E. Coli O157: H7. Methods The virulence genes of Shiga-like toxin(SLT) and rfbE were specifically amplified by 2 sets of primers. The target gene fragments of the PCR assay were 224 bp and 499 bp, respectively. Results Analysis of 37 strains demonstrated that this PCR system was specific. The detection limit of the PCR was 4 CFU/ml. Conclusion These results indicated that the multiplex PCR-DHPLC assay can be used for specific and sensitive detection of E. Coli O157:H7.
4.Relationship between CD158j expression and phosphorylated ERK levels in CD4 + CD28null T cells in cerebral infarction patients with carotid atherosclerosis
Baoguo CHEN ; Rui ZHENG ; Weihua YAN ; Qiuyue CHEN ; Weiwu SHI ; Boli LI
Chinese Journal of Microbiology and Immunology 2011;(3):269-272
Objective To investigate the relationship between CD158j expression and phosphorylated ERK (p-ERK) in CD4+ CD28null T cells in cerebral infarction (CI) patients- with carotid atherosclerosis and its effects on carotid atherosclerotic plaque stability. Methods Percentage of peripheral CD4+ CD28null and the expression of CD158j and perform on CD4+ CD28null cells was analyzed with flow cytometry in 106 CI patients with carotid atherosclerosis, 33 CI patients with normal carotid arteries and in 50 normal controls, respectively; p-ERK expression was assayed with flow cytometry in 36 CI patients with unstable plaque, and serum IFN-γ was detected with ELISA. The intima-media thickness (IMT) of bilateral carotid arteries in all subjects was confirmed by the colour Doppler ultrasonograph imagingResults Percentage of the CD4+ CD28null T cells, expression of CD158j and perform on CD4+ CD28null T cells and the serum IFN-γ levels was dramatically higher in CI patients than that in normal controls, respectively (all P <0.01), which was decreased in an order of CI patients with patients with unstable plaque, stable plaque, carotid artery IMT and with normal carotid artery. A strong positive correlation was observed between the CD158j expression and degree of p-ERK in CI patients with unstable plaque (P < 0. 01). Conclusion CD4+ CD28null T cells were significantly increased in CI patients with carotid atherosclerosis. CD158j might up-regulate p-ERK expression and induce the proliferation of the CD4+ CD28nullT cells; consequently, higher cytokine production such as IFN-γ produced by CD4+ CD28null T cells may cause the formation of unstable plaque.
5.Effects of sevoflurane on hippocampal neurogenesis in dentate gyrus of mice of different ages
Junke JIA ; Feng ZHENG ; Zongze ZHANG ; Qiuyue YANG ; Ting CHEN ; Mian PENG ; Yanlin WANG ; Chang CHEN
Chinese Journal of Anesthesiology 2018;38(7):817-820
Objective To evaluate the effects of sevoflurane on hippocampal neurogenesis in den-tate gyrus (DG) of mice of different ages. Methods Ninety-six SPF healthy male C57BL∕6 mice, aged 2 weeks, 6 weeks, 9 months and 20 months (24 mice for each age, 12 mice for each group), were divided into 2 groups (n=48 each) using a random number table method: control group (group C) and sevoflurane group (group S). Group S inhaled 3. 0% sevoflurane for 2 h once a day for 3 consecutive days, while group C inhaled the mixture of air and O2. Six mice of each age were selected, and 5′-bromo-2′-deoxyuridine (BrdU) 50 mg∕kg was intraperitoneally injected immediately before and after inhalation once a day for 3 consecutive days in two groups. Mice were sacrificed at 24 h after the last inhalation (T1), brains were re-moved and hippocampi isolated for determination of the number of nestin and doublecortin ( DCX) positive cells in DG by immunohistochemistry. Mice were sacrificed at 4 weeks after the last inhalation ( T2), brains were removed and hippocampi isolated for determination of the number of neuronal nuclei antigen (NeuN)∕BrdU and glial fibrillary acid protein ( GFAP )∕BrdU positive cells by immunofluorescence. Re-sults Compared with group C, the number of nestin and DCX positive cells was significantly reduced at T1, and the number of NeuN∕BrdU and GFAP∕BrdU positive cells was reduced at T2in mice of 2 weeks and 20 months old (P<0. 05), and no significant change was found in the indices mentioned above in mice of 6 weeks and 9 months old in group S ( P>0. 05). Conclusion Three percent sevoflurane can inhibit hipp-ocampal neurogenesis in DG of immature and old mice and exerts no influence on hippocampal neurogenesis in DG of juvenile and adult mice.
6.A Novel Recombinant BCG Vaccine Encoding Eimeria tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis.
Qiuyue WANG ; Lifeng CHEN ; Jianhua LI ; Jun ZHENG ; Ning CAI ; Pengtao GONG ; Shuhong LI ; He LI ; Xichen ZHANG
The Korean Journal of Parasitology 2014;52(3):251-256
A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased CD4+ and CD8+ cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.
Adjuvants, Immunologic/genetics/*metabolism
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Administration, Intranasal
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Animals
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Antigens, Protozoan/genetics/*immunology
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BCG Vaccine/administration & dosage/*genetics
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CD4-Positive T-Lymphocytes/immunology
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CD8-Positive T-Lymphocytes/immunology
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Chickens
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Coccidiosis/*prevention & control
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Disease Models, Animal
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Drug Carriers/administration & dosage
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Eimeria tenella/genetics/*immunology
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Genetic Vectors
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Injections, Subcutaneous
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Interleukin-2/genetics/*metabolism
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Protozoan Vaccines/administration & dosage/genetics/*immunology
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Spleen/immunology
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Vaccines, Synthetic/administration & dosage/genetics/immunology
7. Association between variations in protocadherin 15 gene and occupational noise-induced hearing loss
Xiangrong XU ; Qiuyue YANG ; Jie JIAO ; Yuxin ZHENG ; Lihua HE ; Shanfa YU ; Guizhen GU ; Guoshun CHEN ; Wenhui ZHOU ; Hui WU ; Yanhong LI ; Huanling ZHANG ; Zengrui ZHANG
Chinese Journal of Preventive Medicine 2017;51(1):20-26
Objective:
The aim of this study was to investigate whether genetic variability in the protocadherin 15 (PCDH15) gene may correspond with increased susceptibility to noise-induced hearing loss (NIHL) in a Chinese population.
Methods:
A nested case-control study was performed that followed a cohort of 7 445 noise-exposed workers in a steel factory of Henan province in China from January 1, 2006 to December 31, 2015. In this study, 394 cases who had an average hearing threshold of more than 40 dB (A) in high frequency were defined as the case group, and 721 controls who had an average hearing threshold of less than 35 dB (A) in high frequency and less than 25 dB (A) in speech frequency were defined as the control group. A questionnaire was completed by participants and a physical test was also conducted. SNP genotyping was performed using the SNPscanTM Kit. Multivariate unconditional logistic regression additive models were used to analyze the genotypes in different groups, and the association with NIHL. Unconditional logistic regression models were used to assess the associations between the genotypes and NIHL.
Results:
The average age of study participants was (40.5±8.3) years and the median number of noise-exposed working years
8. Association between eye absent homolog 4 gene polymorphisms and occupational noise-induced hearing loss
Qiuyue YANG ; Xiangrong XU ; Jie JIAO ; Yuxin ZHENG ; Lihua HE ; Shanfa YU ; Guizhen GU ; Guoshun CHEN ; Wenhui ZHOU ; Hui WU ; Yanhong LI ; Huanling ZHANG ; Zengrui ZHANG
Chinese Journal of Preventive Medicine 2017;51(1):27-33
Objective:
To identify the association between genetic polymorphisms in the eye absent homolog 4 (EYA4) gene and noise-induced hearing loss (NIHL).
Method:
A nested case control study was conducted based on a cohort of noise-exposed subjects. In total, 292 cases were selected from a steel factory from 6 297 subjects during Jan 1, 2006 to Dec 12, 2015,who had an average hearing threshold of more than 40 dB(A); 584 matched control subjects for each case were designated on the basis of matched criteria including same gender, age (±5 years) and duration of exposure to noise (±2 years). What's more, the control group had an average hearing threshold of less than 35 dB(A) in high frequency and less than 25 dB(A) in speech frequency. Four single nucleotide polymorphisms (SNPs) of the EYA4 gene were genotyped using a SNPscanTM multiplex SNP genotyping kit. Hardy-Weinberg equilibrium tests were performed using a χ2 test for goodness-of-fit for each SNP among the control group, and the effects of genotypes of the EYA4 gene on NIHL were analyzed by logistic regression. The haplotypes were established and their frequencies in the two groups were assessed using Haploview 4.2 and Phase 2.1 software, and interactive effects between haplotypes and cumulative noise exposure were analyzed.
Results:
The average age of the subjects was (40.1±8.4) years and the average number of noise-exposed working years was 20.3 (8.4, 27.3) years. The range of noise exposure levels and the cumulative noise exposure were 80.2- 98.8 dB (A) and 86.6- 111.2 dB(A) · year, respectively. After adjustment for covariates including height, blood pressure, drinking status and smoking status, in the noise intensity>85 dB (A) group, subjects carrying the rs3813346 TT genotype had a higher NIHL risk than those carrying the GG genotype, and the adjusted
9. Investigation into the relationship between mitochondrial 12 S rRNA gene, tRNA gene and cytochrome oxidase Ⅱ gene variations and the risk of noise-induced hearing loss
Jie JIAO ; Guizhen GU ; Guoshun CHEN ; Yanhong LI ; Huanling ZHANG ; Qiuyue YANG ; Xiangrong XU ; Wenhui ZHOU ; Hui WU ; Lihua HE ; Yuxin ZHENG ; Shanfa YU
Chinese Journal of Preventive Medicine 2017;51(1):34-40
Objective:
To explore the relationship between mitochondrial 12 S rRNA gene variation, tRNA gene variation and cytochrome oxidase Ⅱ gene point mutations and the risk of noise-induced hearing loss (NIHL).
Methods:
A nested case-control study was performed that followed a cohort of 7 445 noise-exposed workers in a steel factory in Henan province, China, from January 1, 2006 to December 31, 2015. Subjects whose average hearing threshold was more than 40 dB(A) in high frequency were defined as the case group, and subjects whose average hearing threshold was less than 35 dB(A) in high frequency and less than 25 dB (A) in speech frequency were defined as the control group. Subjects was recruited into the case group (