Objective To isolate new conotoxins of Conus textile from the South China Sea,provide new conopeptides for drug discovery and design, and investigate the effects of living environment on toxin secretion by comparing the compositions of the conotoxins of this species from different areas. Methods Crude conotoxins were purified by Sephadex G-25 and RP-HPLC, the amino acid sequences of the purified peptides were determined. Results More than 10 conotoxins were isolated from Conus textile, 4 conotoxins were firstly isolated and 6 conotoxins had the similar sequence as the reported peptides. Conclusion Many M-family conotoxins were isolated from Conus textile, the main compositions of the venom of Conus textile from the South China Sea were similar to those from Cebu and Marinduquc island in Philippines.