Objective To summarize the experience of perioperatively nursing patients with Hirschsprung’s disease after laparoscopic-assisted improved Soave operation to improve nursing experience.Method Psychological nursing to both patients and their families,well-planned preoperative preparation,postoperative nutritional support and perineal nursing were done.Result Sixty-six patients got recovered and discharged except one who quitted treatment.Conclusion Careful nursing before and after operation is critical for the prevention of complications and successful manipulation of aparoscopic-assisted improved Soave operation.

Objective: To optimize the Shenzhen universal infant hepatitis B immunization method. Methods: To analyze the long effectiveness of different immunization strategies and disease transition after hepatitis B virus infection by using cost-effectiveness method through decision-tree and Markov models among the Shenzhen newborns cohort in 2010 based on the local parameters. Results:Through the current strategies, the program of screening all pregnant women for HBsAg and vaccinating newborns of single-positive mothers combing with HBIG and vaccine would not only prevent more new cases, hepatic carcinoma and related deaths; but also gain more living years and QALYs. From the social prospect, it will save about RMB 40 million compared with the current program. Conclusion: It is suggested that Shenzhen Government should amend the current universal infant hepatitis B immunization program to gain more economic and social benefits.

Several proteins associated with apoptosis were analyzed to investigate the progress of presbycusis based on cell apoptosis. Four proteins of caspase-3, XIAP, Sir2 T1 and cytochrome C which were associated with apoptosis were selected. Influence and progress of four proteins on presbycusis were analyzed. Apoptosis is the key factor which causes presbycusis, including apoptosis of cochlear hair cells, cochlear spiral ganglion cells and primary auditory cortical neuron. There is a close relationship between the four proteins and apoptosis of hearing related cells. These four kinds of protein factors played a role in the progress of presbycusis and are expected to serve as a target for the treatment of presbycusis.

Objective:To investigate the clinical significance of soluble thrombomdulin (sTM)、endothelial protein C receptor (sEPCR) and von Willebrand factor (vWF) in patients with renal transplantation and reject reaction.Methods:The plasma levels of sTM, sEPCR and vWF were successively determined by double antibody sandwich enzyme linked immunoadsordent assay (ELISA) in 42 patients with renal transplantation and reject reaction, and were compared with normal groups.Results:The levels of sTM, sEPCR and vWF in postoperative of renal transplantation patients were higher than those in preoperative ( P 0 05). In patients with renal transplantation, the levels of sTM, sEPCR and vWF each have positive correlation(r=0 595 P

ObjectiveThis study was to explore the feasibility of using the orthogonal plane dose formed by the coronal and sagittal plane to verify the volumetric modulated arc therapy (VMAT) plan.MethodsThe VMAT plans of 12 patients were included in this study.The orthogonal plane dose formed by the coronal and sagittal plane were measured based on the combination of 2D ionization chamber array and multicube phantom,and the point dose were measured based on a multiple hole cylindrical phantom attached with two 0.125 cm3 ionization chamber probes.ResultsIn the measurement of the point dose,the average error was 1.5％ in high dose area ( more than 80％ of maximum),and 1.7％ in low dose area ( less than 80％ of maximum),respectively.The discrepancy of point dose measurement was 1.3％ between the 2D ionization chamber array and the VMAT planning system.In the measurement of the orthogonal plane dose,the pass rate of γ were 93.7％ for 2％/2 mm and 97.2％ for 3％/3 mm.ConclusionIt is reliable for using the orthogonal plane dose formed by the coronal and sagittal plane to verify the VMAT plan.

OBJECTIVE To solve the problem of the dynamic disinfection when repollution of the air in the key(departments) of hospital exists.METHODS The germicidal effecacy using the dynamic and undynamic disinfection of Kangaking Air Disinfector in the key departments was detected.RESULTS The total air germ was probably zero after undynamic infection.(After) dynamic infection,the total air germ conformed the national standardⅡand Ⅲ.Many kinds of department′s rate of infection descended than ever.CONCLUSIONS The effective disinfection of Kangaking YKX/G,YDX/Y Type Air Disinfector is obvious.The disinfector is a new anti-infective method and one of the most important way to prevent the hospital infection.

The key factor of chemotherapy for brain disorders is to penetrate brain blood barrier(BBB). Receptor mediated en-docytosis is one of the mechanisms for drug carriers to cross the BBB. Angiopep-2(ANG)can specifically bind to low density lipopro-tein receptor-related proteins 1(LRP-1)overexpressed on BBB and glioma cells,thus exhibits a higher LRP-mediated BBB penetra-tion capability. Its targeting efficiency is higher than that of other targeting molecules such as transferrin and(TF)lactoferrin(LF). This paper introduces the structure and function of ANG,and its application in brain targeting delivery systems,in order to provide references for research and development of brain targeting preparations and guidance for therapy of brain diseases.

Objective To investigate the changes of B lymphocyte subsets ( naive B cells, memory B cells and plasmablasts) in peripheral blood of patients with rheumatoid arthritis ( RA) and their correla-tions with the clinical manifestation and laboratory indexes.Methods Sixty-six patients with RA were di-vided into two groups including the group with active RA and the group with inactive RA according to the dis-ease activity score in 28 Joints (DAS28).A control group with healthy subjects was set up accordingly.The distributions of B lymphocyte subsets in peripheral blood samples were detected with flow cytometry analysis and their correlations with clinical manifestations and laboratory indicators were analyzed.Results ( 1 ) Compared with healthy subjevts, the mean fluorescence intensities ( MFIs) of CD19 and the percentages of memory B cells in peripheral blood of the patients with RA were significantly decreased, while the percenta-ges of naive B cells were increased (P<0.05).The percentages of plasmablasts in the patients with active RA were significantly increased as compared with those of healthy subjects and the patients with inactive RA (P<0.05).(2) The percentages of plasmablasts in peripheral blood of the patients with RA were positively correlated with the joint tenderness count, joint swelling count and joint swelling index (P<0.05).(3) A positive correlation was found between the MFIs of CD19 and the erythrocyte sedimentation rates ( ESRs ) among the patients with RA.The percentages of plasmablasts were positively correlated with C reaction pro-tein (CRP) and anti-cyclic citrullinated peptide (anti-CCP) antibody (P<0.05).(4) The percentages of plasmablasts were also positively correlated with the DAS28 among the patients with RA ( R2=0.343, P<0.01).Conclusion The distributions of B lymphocyte subsets varied among the patients in different stages of RA, which were related to the patient′s clinical symptoms and laboratory indexes.The study suggested that different subsets of the B lymphocytes might play an important role in the pathological process of RA.

Objective To investigate the expression and its clinical significance of bronchial asthma treat-ment in different periods of peripheral blood lymphocyte CD +19 CD +23 .Methods Peripheral blood CD +19 CD +23 expres-sion and IgE levels in serum of 28 cases of children with bronchial asthma and 28 healthy children was detected by electrochemical detection and flow cytometry assay method,the detection results were analyzed.Results The results of serum CD +19 CD +23 in children with asthma,the level of total IgE were (32.51 ±5.08)%,(995.22 ±576.33)IU /L respectively,which were higher than those of the control group(21.29 ±8.68)%,(100.85 ±36.43)IU /L,there were significant differences between the two groups(t =5.846,8.334,all P <0.01);treatment after 1 months,3 months, 6 months CD +19 CD +23 expression rates were (29.37 ±4.82)%,(26.20 ±4.58)%,(23.69 ±4.54)%,total IgE lev-els were (745.41 ±452.89),IU /L(553.23 ±345.26),IU /L(405.87 ±267.96)IU /L,before and after treatment were decreased,the differences were statistically significant(CD +19 CD +23 :F =3.367,IgE:F =26.740,P <0.01 ). Conclusion Peripheral blood lymphocyte CD +19 CD +23 and IgE can reflect the condition of the asthma children,but compared to IgE,CD +19 CD +23 can better reflect the activation of eosinophils and airway inflammation,serum CD +19 CD +23 levels can be used as a diagnosis of asthma,disease status and guide the indicator to determine the treatment of inflammation.

AIM:To investigate the effects of survivin inhibitor YM155 {4,9-dihydro-1-(2-methoxyethyl)-2-methyl-4,9-dioxo-3-(2-pyrazinylmethyl)-4,9-dihydro-1H-naphtho[2,3-d]imidazolium bromide} on the apoptosis, mito-chondrial membrane potential (Δψm) and cytochrome C (Cyt C) of retinoblastoma Y79 cells, and to analyze the mitochon-drial mechanisms of apoptosis .METHODS:Y79 cells were cultured in vitro and treated with YM155 at concentrations of 0, 0.5, 1, 2, 4 and 8 nmol/L.The cells in control group were treated without YM 155.The proliferation of Y79 cells were measured by CCK-8 assay and bromodeoxyuridine ( BrdU) labeling assay .Y79 cells were randomly divided into 4 groups:control group ( with equal volume of RPMI-1640 nutrient medium ) , positive control group ( 10 nmol/L topotecan ) , low-dose (1 nmol/L) YM155 group and high-dose (2 nmol/L) YM155 group.The effects of YM155 on the apoptosis, the changes of Δψm , the mitochondrial distribution and the protein level of Cyt C in the Y 79 cells were evaluated by flow cytom-etry with Annexin V-FITC/PI staining, JC-1 staining, immunofluorescence analysis and Western blot , respectively.RE-SULTS:Compared with control group , YM155 significantly inhibited the proliferation of Y 79 cells and induced apoptosis (P<0.05).YM155 significantly reduced Δψm of the Y79 cells, promoted Cyt C which released from mitochondria to the cytosol and reduced the protein level of Cyt C in the mitochondria (P<0.05).CONCLUSION:YM155 inhibits Y79 cell proliferation and induces apoptosis , and the possible mechanisms may be involved in the mitochondrium-mediated apoptotic pathway .