Objective To research paramagnetic contrast media(PCM)dose,injection speed and imaging time etc in low field MRI.Methods 406 patients with lesions spreading all over the body received Gadolinium-DTPA-enhanced scan by bolus injection with the dose of 0.1～0.15 mmol/kg within 60 seconds,and the effects of contrast-enhanced were observed.Results Among 406 patients lesions,337 cases(83%)were enhanced,9 undiscovered lesions displayed enhancement on the precontrast views,5 cases were discovered new lesions in delaying enhancement scan.Conclusion It is safe to apply the above mentioned dose and injection speed in clinic.The side-effects is small,and the development effect is good.It is especially important for lesions involving the structure of the blood brain barrier(BBB).

Objective To study the effect of hepatitis B vin1s X-interacting protein (HBXIP) on the proliferation,migration,and invasion of ACC-2 cells,and the possible mechanism of the PI3K/Akt signaling pathway involved.Methods The chemically synthesized HBXIP-siRNA plasmid was transfected into the ACC-2 cells.RT-PCR and Western blotting were performed to detect the expression of HBXIP in the ACC-2 cells.Cell proliferation was measured via MTT assay.The invasive and migratory abilities of the ACC-2 cells were evaluated via the transwell chamber assay and scratch test,respectively.Western blotting also detected the impact of HBXIP-siRNA on Akt,p-Akt,PI3K,p-PI3K,and S100A4 protein expression.Results HBXIP was highly expressed and HBXIP-siRNA was successfully transfected in ACC-2 cells.MTT results showed that the number of surviving cells in the experimental group was significantly lower than that in the control group (P＜0.05).The scratch test results showed that the mobility of the experimental group was significantly lower than that of the control group (P＜0.01).The transwell assay showed that the rate of cell invasion of the experimental group was significantly lower than that of the control group (P＜0.01).Finally,Western blotting results revealed that the expression of p-Akt,p-PI3K,and S 100A4 was relatively decreased in the experimental group when compared to that in the control group.Conclusion Silencing the HBXIP gene inhibited ACC-2 proliferation,invasion,and migration.