1.Determination of three components in Cang'ebiyan Tablets
Liuchun WU ; Qiuxiang JIANG ; Xiaoqing KONG
Chinese Traditional Patent Medicine 1992;0(03):-
AIM: To establish method of determing baicalin,chlorogenic acid and chlorpheniramine maleate in Cang'ebiyan Tablets(Fructus Xanthii,Herba Cemtipedae,Radix Astragali,Flos Chrysanthemi indici,etc). METHODS: The VP-ODS C_(18) column(4.6 mm?250 mm,5 ?m) was used with a mobile phase of acetonitrile-0.4%H_3PO_4 0-9 min(14∶86),9-20 min(28∶72) gradient elution,the wavelength of detector was set at 0-9 min 327 nm for determining chlorogenic acid content,9-20 min 280 nm for determining baicalin.The VP-ODS C_(18) column(4.6 mm?250 mm,5 ?m) was used with a mobile phase of acetonitrile-0.5% sodium dodecylsulfate-H_3PO_4,the wavelength of detector was set at 262 nm for determining chlorpheniramine maleate. RESULTS: The calibration curves were linear in the range of 0.21-3.14 ?g/mL(r=0.999 9) for baicalin,in the range of(0.05-0.81 ?g/mL)(r=0.999 9) for chlorogenic acid,in the range of 0.10-1.57 ?g/mL(r=0.999 9) for chlorpheniramine maleate,respectively.The average recoveries were 102.44%,RSD=0.57%(n=6);(98.68%,)RSD=0.30%(n=6);99.46%,RSD=0.70%(n=6),respectively. CONCLUSION: The method is simple,realizable and reproducible and can be used effectively for the quality control of Cang'ebiyan Tablets.
2.Quality standard improvement of Compound Isodon Rernifolia (D.Don) Kudo Tablets
Liuchun WU ; Zilong QIN ; Qiuxiang JIANG
Chinese Traditional Patent Medicine 1992;0(07):-
AIM: HPLC-ELSD method was established to investigate the content of oleanolic acid and ursolic acid in Compound Isodon Rernifolia(D.Don) Kudo Tablets. METHODS: The determination of oleanolic acid and ursolic acid was conducted by HPLC-ELSD using a Agilent Eclipse XDB-C_(18)(4.6 mm?250 mm,5 ?m) column;The mobile phase consisted of methanol-0.5% ammonium acetate solution(82∶18).The flow rate was 1.0 mL/min.The column temperature was set at 30 ?C.The drift tube temperature was set at 85 ?C.The gas flow rate was 2.0 L/min. RESULTS: The linear ranges of oleanolic acid and ursolic acid were 0.45-2.69 ?g(r=0.999 9) and 0.63-3.75 ?g(r=0.999 3),respectively.The average recoveries of oleanolic acid and ursolic acid were(100.3%)(RSD=1.32%) and 100.6%(RSD=1.66%),respectively. CONCLUSION: The method is simple,accurate,and specific which is better than the original one.
3.HBeAg Serving as a Supervising and Controlling Index of HBV Spread During Operation:A Practicality Study
Shuangwang YANG ; Haiping YANG ; Minlan JIANG ; Lin JIN ; Xuejun LAI ; Qiuxiang LIU
Chinese Journal of Nosocomiology 2006;0(08):-
OBJECTIVE To investigate whether the HBV variant of HBeAg negative influences the practicality of hepatitis Be antigen(HBeAg) serving as a supervising and controlling index of HBV spread during operation.METHODS On the basis of that ascertain the relationship between HBV variant of HBeAg negative and source of HBV infection during operation,to investigate whether the HBV variant influenced the practicality of HBeAg serving as a supervising and controlling index of HBV spread during operation.RESULTS The HBV infectivity from low to high was HBeAg negative operated patients
4.Prevention Measures Against HBV Infection among Surgeons
Haiming SHI ; Shuangwang YANG ; Weiqiong GUO ; Minlan JIANG ; Haiping YANG ; Qiuxiang LIU ; Xuejun LAI
Chinese Journal of Nosocomiology 1994;0(01):-
OBJECTIVE To explore the prevention measures against HBV infection among surgeons.METHODS On the basis of the comparison of the current prevention measures HBV infection during operation in the Eastern and Western countries,new prevention measures that HBeAg serves as a supervising and controlling index of HBV were studied.RESULTS The concentration HBV of HBsAg negative,HBeAg negative and HBeAg positive patients were(100-104),(100-106) and(102-109) ID/ml,respectively.HBV infective threshold value of single layer gloves was 105ID/ml,and that of double layer gloves was 106ID/ml.The viral concentration of all the HBsAg negative patients did not reach the infective threshold value of single layer gloves.83.88% HBeAg positive patients reached the threshold value,from them 80.65% was protected by HBV vaccine and 3.23% remainders exceeded the protection.CONCLUSIONS HBeAg serving as a supervising and controlling index of HBV infection can be an effective indicator which can reduce the risk of infection.It is more suitable for the current condition of our country.
5.A case report on irritable bowel syndrome treated with low fermentable oligosaccharides, disaccharides, monosaccharides, and polyols diet
Yutang REN ; Ruifeng WANG ; Xutong YU ; Xuan JIANG ; Xiaojuan GUO ; Xueru HUO ; Xiaofang YING ; Qiuxiang LU ; Bo JIANG
Chinese Journal of Clinical Nutrition 2023;31(2):113-116
Irritable bowel syndrome (IBS) is characterized by abdominal pain associated with changes in defecation frequency and blood folate level. FODMAP stands for fermentable oligosaccharides, disaccharides, monosaccharides and polyols. High-FODMAP diet could elicit or exacerbate IBS-associated bowel symptoms, which is inadequately recognized among gastroenterologists in China. Here we report an IBS case, focusing on the association between high-FODMAP diet and bowel symptoms and the intervention of low-FODMAP diet.
6.Application of Huangkui Capsules in Diabetic Kidney Disease: A Review
Jia LUO ; Beile JIANG ; Qiuxiang HE ; Shilong LU
Chinese Journal of Experimental Traditional Medical Formulae 2025;31(7):314-324
Diabetic kidney disease (DKD) is a kidney disease caused by hyperglycemia,which is one of the most common microvascular complications of diabetes. Due to the high incidence of diabetes,the incidence of DKD has also increased year by year,and DKD has become a global public health problem. The pathogenesis of DKD is related to mechanisms such as oxidative stress,inflammation,renal fibrosis,and decreased mitophagy activity,which are developed under a variety of complex mechanisms. In traditional Chinese medicine,it is believed that the incidence of DKD is closely related to damp heat. Therefore,it is necessary to grasp the treatment method of clearing heat and removing dampness in clinical medication. Huangkui Capsules (HKC) have the effect of clearing damp heat,detoxifying, and detumescence. Because of its unique curative effect on DKD,HKC is often used in the treatment of DKD. HKC plays a role in the treatment of DKD with a variety of pharmacokinetic and pharmacodynamic processes. In many laboratory studies,it has been found that the specific mechanisms of HKC in the treatment of DKD include increasing mitophagy,reducing mitochondrial damage,reducing renal fibrosis,controlling inflammatory response,and inhibiting oxidative stress,which can achieve the purpose of reducing renal damage and promoting renal function. Some clinical studies have also verified that the application of HKC alone can exert renal protective function through anti-inflammatory,anti-oxidative stress,anti-renal fibrosis effects,as well as reduction of urinary protein. Since DKD is not a single injury of renal function,it is often accompanied by problems in blood pressure,blood lipids,blood circulation,body immunity, and other aspects. Therefore,the combination of HKC with other drugs can often achieve more comprehensive results,improve the advantages of various drugs,and improve the therapeutic effect. The combination of drugs such as antihypertensive,lipid-lowering, vascular circulation improvement,immunity inhibition,and anti-oxidative stress with HKC has achieved good results. In addition,HKC is often used in combination with other Chinese patent medicines in clinics. The application of HKC in the treatment of DKD has made some progress,but there are still many places worthy of further study,and the research on the mechanism of HKC is not comprehensive enough. The research on its long-term effect and safety in clinical application is relatively lacking,and the drug variety is relatively single when combined with certain drugs. These problems deserve further attention. Finally,it is necessary to pay attention to the promotion and application of HKC in clinical practice so that HKC can be better applied in clinical practice and better solve practical problems for patients.
7.Application of Huangkui Capsules in Diabetic Kidney Disease: A Review
Jia LUO ; Beile JIANG ; Qiuxiang HE ; Shilong LU
Chinese Journal of Experimental Traditional Medical Formulae 2025;31(7):314-324
Diabetic kidney disease (DKD) is a kidney disease caused by hyperglycemia,which is one of the most common microvascular complications of diabetes. Due to the high incidence of diabetes,the incidence of DKD has also increased year by year,and DKD has become a global public health problem. The pathogenesis of DKD is related to mechanisms such as oxidative stress,inflammation,renal fibrosis,and decreased mitophagy activity,which are developed under a variety of complex mechanisms. In traditional Chinese medicine,it is believed that the incidence of DKD is closely related to damp heat. Therefore,it is necessary to grasp the treatment method of clearing heat and removing dampness in clinical medication. Huangkui Capsules (HKC) have the effect of clearing damp heat,detoxifying, and detumescence. Because of its unique curative effect on DKD,HKC is often used in the treatment of DKD. HKC plays a role in the treatment of DKD with a variety of pharmacokinetic and pharmacodynamic processes. In many laboratory studies,it has been found that the specific mechanisms of HKC in the treatment of DKD include increasing mitophagy,reducing mitochondrial damage,reducing renal fibrosis,controlling inflammatory response,and inhibiting oxidative stress,which can achieve the purpose of reducing renal damage and promoting renal function. Some clinical studies have also verified that the application of HKC alone can exert renal protective function through anti-inflammatory,anti-oxidative stress,anti-renal fibrosis effects,as well as reduction of urinary protein. Since DKD is not a single injury of renal function,it is often accompanied by problems in blood pressure,blood lipids,blood circulation,body immunity, and other aspects. Therefore,the combination of HKC with other drugs can often achieve more comprehensive results,improve the advantages of various drugs,and improve the therapeutic effect. The combination of drugs such as antihypertensive,lipid-lowering, vascular circulation improvement,immunity inhibition,and anti-oxidative stress with HKC has achieved good results. In addition,HKC is often used in combination with other Chinese patent medicines in clinics. The application of HKC in the treatment of DKD has made some progress,but there are still many places worthy of further study,and the research on the mechanism of HKC is not comprehensive enough. The research on its long-term effect and safety in clinical application is relatively lacking,and the drug variety is relatively single when combined with certain drugs. These problems deserve further attention. Finally,it is necessary to pay attention to the promotion and application of HKC in clinical practice so that HKC can be better applied in clinical practice and better solve practical problems for patients.
8. Inhibition of CD4+T cell infiltration by interleukin-10 competent B cells in periodontitis tissues
Guoqin CAO ; Xu ZHANG ; Yan ZHAO ; Siqi ZHAO ; Guocui DONG ; Qiuxiang GAO ; Zuomin WANG ; Jiang LIN
Chinese Journal of Stomatology 2019;54(8):553-560
Objective:
To study the immune regulation function of high expressing interleukin-10 (IL-10) in B cells on CD4+T-cells in periodontitis mouse model.
Methods:
Twenty-four 7-weeks-old female C57BL/6 mice were randomly and equally assigned into 4 groups: the healthy control group (HC group,
9.High-throughput screening of SARS-CoV-2 main and papain-like protease inhibitors.
Yi ZANG ; Mingbo SU ; Qingxing WANG ; Xi CHENG ; Wenru ZHANG ; Yao ZHAO ; Tong CHEN ; Yingyan JIANG ; Qiang SHEN ; Juan DU ; Qiuxiang TAN ; Peipei WANG ; Lixin GAO ; Zhenming JIN ; Mengmeng ZHANG ; Cong LI ; Ya ZHU ; Bo FENG ; Bixi TANG ; Han XIE ; Ming-Wei WANG ; Mingyue ZHENG ; Xiaoyan PAN ; Haitao YANG ; Yechun XU ; Beili WU ; Leike ZHANG ; Zihe RAO ; Xiuna YANG ; Hualiang JIANG ; Gengfu XIAO ; Qiang ZHAO ; Jia LI
Protein & Cell 2023;14(1):17-27
The global COVID-19 coronavirus pandemic has infected over 109 million people, leading to over 2 million deaths up to date and still lacking of effective drugs for patient treatment. Here, we screened about 1.8 million small molecules against the main protease (Mpro) and papain like protease (PLpro), two major proteases in severe acute respiratory syndrome-coronavirus 2 genome, and identified 1851Mpro inhibitors and 205 PLpro inhibitors with low nmol/l activity of the best hits. Among these inhibitors, eight small molecules showed dual inhibition effects on both Mpro and PLpro, exhibiting potential as better candidates for COVID-19 treatment. The best inhibitors of each protease were tested in antiviral assay, with over 40% of Mpro inhibitors and over 20% of PLpro inhibitors showing high potency in viral inhibition with low cytotoxicity. The X-ray crystal structure of SARS-CoV-2 Mpro in complex with its potent inhibitor 4a was determined at 1.8 Å resolution. Together with docking assays, our results provide a comprehensive resource for future research on anti-SARS-CoV-2 drug development.
Humans
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Antiviral Agents/chemistry*
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COVID-19
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COVID-19 Drug Treatment
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High-Throughput Screening Assays
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Molecular Docking Simulation
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Protease Inhibitors/chemistry*
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SARS-CoV-2/enzymology*
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Viral Nonstructural Proteins