1.Subcellular localization and resistance to Gibberella fujikuroi of AtELHYPRP2 in transgenic tobacco.
Qiuxia CHAI ; Benchang LI ; Ziqin XU
Chinese Journal of Biotechnology 2014;30(3):472-484
The subcellular localization and the resistance to fungal pathogen Gibberella fujikuroi of the protein encoded by Arabidopsis AtELHYPRP2 (EARLI1-LIKE HYBRID PROLINE-RICH PROTEIN 2, AT4G12500) were investigated using transgenic tobacco plants. The coding sequence of AtELHYPRP2 was amplified from genomic DNA of Col-0 ecotype. After restriction digestion, the PCR fragment was ligated into pCAMBIA1302 to produce a fusion expression vector, pCAMBIA1302-AtELHYPRP2-GFP. Then the recombinant plasmid was introduced into Agrobacterium tumefaciens strain LBA4404 and transgenic tobacco plants were regenerated and selected via leaf disc transformation method. RT-PCR and Western blotting analyses showed that AtELHYPRP2 expressed effectively in transgenic tobacco plants. Observation under laser confocal microscopy revealed that the green fluorescence of AtELHYPRP2-GFP fusion protein could overlap with the red fluorescence came from propidium iodide staining, indicating AtELHYPRP2 is localized to cell surface. Antimicrobial experiments exhibited that the constitutive expression of AtELHYPRP2 could enhance the resistance of tobacco to fungal pathogen G. fujikuroi and the infection sites could accumulate H2O2 obviously. The basal expression levels of PR1 and the systemic expression levels of PR1 and PR5 in transgenic tobacco plants were higher than that of the wild-type plants, suggesting AtELHYPRP2 may play a role in systemic acquired resistance.
Agrobacterium tumefaciens
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Arabidopsis
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Arabidopsis Proteins
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genetics
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Disease Resistance
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Gibberella
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pathogenicity
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Hydrogen Peroxide
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Plants, Genetically Modified
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microbiology
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Recombinant Fusion Proteins
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genetics
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Tobacco
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genetics
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microbiology
2.Retention force attenuation of 2 kinds of clasps and surface abrasion of the Cr-Co crowns
Xu LAN ; Ying YANG ; Qiuxia YANG
Journal of Practical Stomatology 2014;(2):165-168
Objective:To investigate the the retention force changes of 2 kinds of T bar clasps on Cr-Co crowns and the surface ab-rasion of the crowns.Methods:Standard Cr-Co crowns were made,and standard T bar clasps were made of Cr-Co alloy and Vitalli-um2000 with 0.25 mm and 0.5 mm undercut depth respectively.1 800 times repeated placement and removal(fatigue test)were car-ried out on instron testing machine in or without saliva soak condition.The attenuation curves of the maximum retention force was an-alysed.The surface abrasion of the crowns was observed before and after the fatigue test by SEM.Results:The retention force attenu-ation of the clasps in every group was accord with the logarithm attenuation law.SEMexamination showed that in the same condition, there was less surface abrasion in Vitallium2000 group than that in Cr-Co group.Vitallium2000 clasp was superior to Cr-Co clasp in maintaining the retention force.Conclusion:Vitallium2000 T bar clasp may provide better retention force with less furface abrasion on Cr-Co crown than Cr-Co clasp.
3.Hospital Infections in Patients after Neck Dissection and Nursing Intervention
Qiuxia ZHANG ; Yonggen XU ; Qiaoxia JIN
Chinese Journal of Nosocomiology 2006;0(09):-
OBJECTIVE To investigate risk factors and nursing protective measures controlling hospital infection in patients after neck dissection. METHODS The data of 116 patients after neck dissection from Aug 2000 to Aug 2005 were collected, and the hospital infection condition was analyzed in comparison with non-infection group. RESULTS Hospital infection rate was 18.97% (22/116) and case-time rate was 21.55%(25/116). Of 25 occasions in hospital infections, 48% were infection of operative incision and 24% were infection of respiratory tract. The risk factors for hospital infection in patients with neck dissection might be associated with patient's duration of hospitalization, history of chronic disease , age and invasive operation. CONCLUSIONS There is a high hospital infection incidence rate in patients after neck dissection , it is necessary to monitor high risk patients.
4.Nesfatin-1 Signaling in the Basom Edial Amygdala Modulates the Gastric Distension-sensitive Neurons Discharge and Decreases Gastric Motility by the Arcuate Nucleus
Qiuxia DUAN ; Xiao LUAN ; Feifei GUO ; Xiangrong SUN ; Luo XU
Progress in Modern Biomedicine 2017;17(27):5227-5232,5246
Objective:This study aimed to explore the effects ofnesfatin-1 on gastric distension (GD)-sensitive neurons in the basomedial amygdala (BMA) and the potential mechanism for nesfatin-1 to regulate gastric motility through the arcuate nucleus (Arc).Methods:The projection of nerve fiber and expression of nesfatin-1 were observed by retrograde tracing and fluo-immunohistochemistry staining;The nuclei microinjection and nuclei electrical stimulation,extracellular discharges of single unit neuron were used to observe the effects ofnesfatin-1 on the GD neurons;Gastric motility recording in vivo were used to monitor the effects ofnesfatin-1 on the amplitude of constriction and frequency of gastric motility in conscious rats.Results:NUCB2/Nesfatin-1/fluorogold-double labeled neurons were from ARC to BMA;Nesfatin-1 could excited the firing rate of most of the GD-E neurons (4.25± 1.02 Hz vs.5.32± 1.17 Hz,P<0.01) and decreased the firing rate of most of the GD-I neurons (3.73± 0.92 Hz vs.2.64± 0.86 Hz,P<0.01),inhibited the gastric motility,amplitude and frequency,SHU9119 could weaken the responses induced by nesfaton-1;Electrical stimulation of the Arc,the firing rate of nesfatin-1-induced GD-response neurons (GD-E:5.14± 1.32 Hz vs.6.75± 1.84 Hz,P<0.05;GD-I:2.84± 0.86 Hz vs.4.05± 1.12 Hz,P <0.05) and the gastric amplitude and frequency were increase.Conclusion:It was suggested that nesfatin-1 in the BMA plays an important role in decreasing gastric motility and the Arc may be involved in this regulation process.
5.Analysis of blood screening results before and after changing critical value of ALT
Qiuxia FENG ; Zhongsi YANG ; Haiping PAN ; Li LIU ; Lei XU
International Journal of Laboratory Medicine 2015;(16):2384-2385,2388
Objective To analyze the blood screening results after adjustment of critical value 40 to 50 U/L and to observe the effect of reducing blood scrap rate and to discuss the correlation between ALT and HBV,HCV infection.Methods We screened 2656 blood donors (ALT >40 U/L)by serological and nucleic acid amplification testing(NAT)in Qingdao blood center from 2013 to 2014,and conducted the correlation analysis by chi square test.Results 1 771 cases (66.68%)were ALT 40-50 U/L,including 6 cases of HBsAg ELISA (+),2 cases NAT (+),4 cases NAT(-).In the 8 cases of anti-HCV ELISA (+)samples,4 cases NAT (+),3 cases NAT (-),1 case with positive TP without NAT result.In 885 blood donors with ALT>50 U/L,5 cases were HBsAg-reactive,7 cases were anti-HCV-reactive,and 873 cases were negative.Related statistics showed that there was no signifi-cant difference between ALT and HBV infection (P <0.05),but significant difference was found between ALT and HCV infection (P >0.05).Conclusion The proportion of blood donors with ALT 40-50 U/L is much higher than that with ALT >50 U/L do-nors.Adjustment of the critical value greatly reduces blood scrap rate.Elevated ALT is associated with the infection of HBV but not with HCV.
6.Preliminary study on the biological characters of Ornidazole Slow-Release Membrane
Quanchen XU ; Zhiguo WANG ; Jing DENG ; Qiuxia JI ; Xinbo YU
Chinese Journal of Marine Drugs 2000;0(06):-
Objective To evaluate the biocompatibility and antibacterial activities of the Ornidazole Slow-Release Membrane.Methods 1.The lower lips of 12 rats were sewed into 12 pockets and the pockets were immited with extracting solution of the ornidazole membrane, formaldehyde and normal saline respectively once per day.The specimens were examined histologically 7 days later.2.The dorsal muscles of 16 rats were implanted with the membranes or silk threads,and examined histologically 1 week and 2,4,6 weeks later respectively.3. The antibacterial activity against Streptococcus mutans and Fusobacterium nucleatum was observed on solid culture medium in vitro.Results The animal experiments showed the membranes were not irritative to the oral mucosa.It was found that the tissue reaction of the membranes was similar to that of the silk threads after implanted into dorsal muscles and the membranes had been degraded in the second week.And the membranes had effective antibacterial action against Streptococcus mutans and Fusobacterium nucleatum.Conclusion The Ornidazole Slow-Release Membrane possesses favorable biocompatibility and antibacterial activities.
7.Effect of fluoride concentration on the corrosion behavior of cobalt-chromium alloy fabricated by two different technology processes.
Qiuxia YANG ; Ying YANG ; Han XU ; Di WU ; Ke GUO
West China Journal of Stomatology 2016;34(1):47-53
OBJECTIVEThis study aims to determine the effect of fluoride concentration on the corrosion behavior of cobalt-chromium alloy fabricated by two different technology processes in a simulated oral environment.
METHODSA total of 15 specimens were employed with selective laser melting (SLM) and another 15 for traditional casting (Cast) in cobalt-chromium alloy powders and blocks with the same material composition. The corrosion behavior of the specimens was studied by potentiodynamic polarization test under different oral environments with varying solubilities of fluorine (0, 0.05%, and 0.20% for each) in acid artificial saliva (pH = 5.0). The specimens were soaked in fluorine for 24 h, and the surface microstructure was observed under a field emission scanning electron microscope after immersing the specimens in the test solution at constant temperature.
RESULTSThe corrosion potential (Ecorr) value of the cobalt-chromium alloy cast decreased with increasing fluoride concentration in acidic artificial saliva. The Ecorr, Icorr, and Rp values of the cobalt-chromium alloy fabricated by two different technology processes changed significantly when the fluoride concentration was 0.20% (P < 0.05). The Ecorr, Icorr, and Rp values of the cobalt-chromium alloy fabricated by two different technology processes exhibited a statistically significant difference. The Icorr value of the cobalt-chromium alloy cast was higher than that in the SLM group cobalt-chromium alloy when the fluoride concentration was 0.20% (P < 0.05). The Ecorr, tRp alues of the cobalt-chromium alloy cast were lower htan those of the SLM group cobalt-chromium alloy when the fluoride concentration was 0.20% (P< 0 .05).
CONCLUSIONFluoride ions adversely affected the corrosion resistance of the cobalt-chromium alloy fabricated by two different technology processes. The corrosion resistance of the cobalt-chromium alloy cast was worse than that of the SLM group cobalt-chromium alloy when the fluoride concentration was 0.20%.
Chromium Alloys ; Corrosion ; Fluorides ; Lasers ; Phosphates ; Saliva, Artificial ; Sodium Fluoride
8.Corvis measurement of intraocular pressure and its relationship with corneal biomechanical properties
Kaijian, CHEN ; Qiuxia, KAN ; Ji, BAI ; Guowei, ZHANG ; Duo, XU ; Lina, LIU ; Min, LANG
Chinese Journal of Experimental Ophthalmology 2016;34(2):166-169
Background Corneal biomechanical properties is important in the safety assessment of corneal refractive surgery.Corvis is a new device for measuring corneal biomechanics properties.Objective This study was to observe the correlation among corneal thickness, Corvis intraocular pressure and corneal biomechanical properties with Corvis.Methods A prospective observational study was performed.One hundred and fifty eyes of 75 patients with corneal thickness from 501 μm to 590 μm were divided into three groups according to the corneal thickness:low corneal thickness group (corneal thickness range from 501 μm to 530 μm), middle corneal thickness group (corneal thickness range from 531 μm to 560 μm) ,and high corneal thickness group (corneal thickness range from 561 μm to 590 μm);and 50 eyes of 25 patients for each group.The difference of intraocular pressure, corneal thickness and deformation amplitude (DA) among the three groups were analyzed by one-way ANOVA and the correlation among the groups were analyzed by liner regression.Results The DA in the low corneal thickness group and middle corneal thickness group were significantly higher than that in the high corneal thickness group (P < 0.05).The intraocular pressure was statistically different among the 3 groups (F =9.98, P<0.05).DA was negatively correlated with intraocular pressure and corneal thickness (r=-0.84,-0.33;both at P<0.01), with the linear regression DA =1.69-0.04×IOP (F=366.19, t=-19.14,P<0.01).Conclusions Corneal thickness cannot simply represent the corneal biomechanical properties in the safety assessment of corneal refractive surgery,IOP should be considered.
9.The correlation between the labial (buccal)bone thickness and alveolar crest height in maxillary anterior teeth and premolar zone
Peng ZHANG ; Xiaoshi JIA ; Qiuxia ZHANG ; Xiaoxia WEI ; Mengjie XU ; Meng WANG
Journal of Practical Stomatology 2015;(4):488-492
Objective:To investigate the labial(buccal)bone thickness at alveolar crest zone and alveolar crest height of the maxil-lary anterior teeth and premolars of young adults with normal occlusion.Methods:The alveolar bone of the anterior teeth and premo-lars of 67 eligible Han national young volunteers was scanned by CBCT.Then the facial bone thickness and the distance between the facial alveolar crest and Cemento-enamel Junction(CEJ)of the anterior teeth and premolars were measured and analyzed after recon-struction.Results:The distance between labial (buccal)crest and labial (buccal)CEJ of the maxillary first premolars was the lar-gest(P <0.05);there was a negative correlation between the labial(buccal)crest height and the facial alveolar bone thickness at 2 mm from CEJ toward root derection[(P <0.05),0.6 <|r|<0.8].Conclusion:The labial (buccal)crest of the first premolars was higher than that of other teeth in maxillary aesthetic zone.At alveolar crest zone,when the labial (buccal)bone was thinner,the dis-tance between labial (buccal)crest and labial (buccal)CEJ was larger,and the implant aesthetic risk is higher.
10.Effect of Quetiapine on Brain-derived Neurotrophic Factors in Patients with First-episode Schizophrenia
Jianjun LIU ; Jushui SUN ; Xinhua SHEN ; Hua ZHONG ; Weigang GUO ; Shengli ZHI ; Guangming SONG ; Qiuxia XU
Herald of Medicine 2014;(9):1185-1187
Objective To investigate the effects of quetiapine on serum levels of brain-derived neurotrophic factors ( BDNF) and the correlation between BDNF and psychiatric symptoms and cognitive function in patients with first-episode schizophrenia. Methods Eighty patients with first-episode schizophrenia ( treatment group) were treated with quetiapine orally for 4 weeks,at initial dose of 100 mg·d-1 and average dose of (580±120) mg·d-1 . The psychiatric symptoms were evaluated by using the positive and negative syndrome scale ( PANSS) . The cognitive function was assessed by using Wisconsin cards sort test ( WCST) . The serum BDNF level was detected with enzyme-linked immunosorbent assay ( ELISA) . Results The serum level of BDNF was markedly lower in schizophrenic patients before[(13. 72±8. 79) ng·mL-1,P<0. 01] and after treatment[(18. 02±9.06) ng·mL-1,P<0.05]in comparison with normal controls(23. 67±10. 13) ng·mL-1]. After treatment,the PANSS total scores and subscale scores decreased,WCST number of categories and the number of correct answers increased,and the number of wrong answers reduced. There was a positive correlation between the serum BDNF and negative symptoms ( SANS) ( r= 0. 54, P=0. 032),and the number of correct answers. Conclusion The quetiapine significantly increases serum level of BDNF in schizophrenia patients,which correlates positively with improvements in symptoms and cognitive function.