Objective To observe the clinical effect of mass-dissipating acupuncture therapy for relieving gynecologic chronic pelvic pain. Methods Sixty gynecologic chronic pelvic pain patients were equally randomized divided into treatment group and control group. Both groups were treated with enema and oral use of Chinese medicine, and the treatment group received mass-dissipating acupuncture therapy additionally. Seven days constituted one period of treatment, and the treatment lasted 2 periods. After treatment, the patients were followed up for one month. The visual analog scale ( VAS) was used for the scoring of pain before and after treatment and during the follow-up. The quality of life ( QOL) was evaluated with 36-item short-form health survey (SF-36) before treatment and during the follow-up. Results (1) At the end of the treatment, the VAS scores of the two groups were decreased significantly, the difference being significant compared with those before treatment ( P<0.01) . The difference of pre- and post-treatment VAS scores between the two groups had statistic significance ( P<0.01) . The results of follow-up showed that pain VAS scores were still decreased in both groups as compared those at the end of treatment, the difference being significant (P<0.01). (2) The results of follow-up showed that the scores of each dimension of SF-36 were obviously increased in both groups as compared those before treatment (P<0.01), and the increase was obvious in the treatment group (P<0.05 or P<0.01). Conclusion Mass-dissipating acupuncture therapy combined with enema and oral use of Chinese medicine is effective in relieving gynecologic chronic pelvic pain, enhancing therapeutic effect and improving QOL.

Zebrafish is widely used as a model organism in the process of drug discovery. It expresses drug metabolizing enzymes like cytochrome P450 (CYP450), uridine 5'-diphospho-glucuronosyltransferase (UGT) and nuclear receptors like pregnane X receptor (PXR), aryl hydrocarbon receptor (AHR), etc. This article summarized the profiles of main drug metabolizing enzymes and nuclear receptors, and reviewed the advances on xenobiotics metabolism in zebrafish.

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Objective To analyze the best methods for diagnostic scanning and image processing of multi-slice spiral CT (MSCT) in the detection of renal malformations (RM). Methods From June 2008 to Februany 2012, one hundred and four patients with kidney malformation received 64-slice MSCT assay were enrolled in our Hospital. Related materials of the patients were used for retrospective analysis. Diagnostic accuracy of urinary malformation were calculated on the image of axial and MPR, Curve, MIP, VR by MSCT unenhangced scanning, enhangced cortical phase scanning, enhangced parenchymal phase scanning and enhangced excretory phase scanning. Results One hundred and four patients with kidney abnormalities received the MSCT unhangced scan axial and MPR, Curve film, enhangced cortical phase scanning, enhangced parenchymal phase scanning, enhangced excretory phase scanning and MPR, MIP, VR film, respectively. The diagnostic accuracy were as follws: renal dysplasia (72.73%, 81.82%, 81.82%, 72.73%), renal hypoplasia (83.33%, 91.67%, 95.83%, 100%), solitary kidney (100%, 100%, 100%, 100%), ectopic kidney (76.92%, 100%, 100%, 100%), horseshoe kidney (100%, 100%, 100%, 100%),misplaced renal lobe (0%,100%). Conclusion The MSCT with image post-processing techniques in the diagnosis of RM has its unique advantages, MSCT can be preferred as a method for renal malformation examination.

Objective To investigate the expression of long chain non-coding(lnc) RNA LOC285194 in breast cancer tissue and paracancerous tissue and its clinical significance.Methods Forty-two samples of paraffin embedded breast cancer tissue and 16 samples of paraffin embedded paracancerous tissue were selected.The expression of lncRNA LOC285194 in these tissue were detected by using quantitative real-time polymerase chain reaction(PCR).Then its correlation with clinicopathological features was analyzed.Results The expression level of lncRNA LOC285194 in breast cancer tissue was significantly lower that in the paracancerous tissue (P＜0.01);the level of lncRNA LOC285194 in human epidermal growth factor receptor-2(HER2)overexpression tissues was up-regulated compared with HER2 negative breast cancer tissue(P =0.013),there was a positive correlation between them(r=0.385,P=0.012).Conclusion lncRNA LOC285194 may play the role of cancer suppressor gene and may be involved in the generation of breast cancer by HER2 association,which may become a target gene of breast cancer treatment.

Humans ; Oculocerebrorenal Syndrome ; diagnosis

OBJECTIVE To offer better cleaning and less rusted medical instrument,prevent hand injuries,and the incidence of nosocomial infection among healthcare professionals.METHODS Two methods were used in two operating rooms for comparison.A multi-enzyme rinse liquid,the Ruhof rinse liquid was used in the test group.The traditional method was used in the control group.The cleaning level of these two groups were observed.RESULTS The group using Ruhof rinse liquid was much cleaner than the group without using it(P

Objective To observe the effects of Baizhu Huangqi Decoction and its effective-part prescription on mice ulcerative colitis(UC).Methods The mice UC model was induced by clyster with 2,4,6-trinitrobenzenesulfonic acid(TNBS)and the effects were comprehensively evaluated by disease activity index,the macroscopic and histological assessment of colon mucosa damage and the activity of myeloperoxidase(MPO).Results Both Baizhu Huangqi Decoction and its effective-part prescription had effects on disease activity index(DAI),inflammation index and MPO activity.The effect of effective-part prescription was better than that of UC model group(P

Objective To evaluate the clinical efficacy of berberine for the treatment of insulin resistance (IR) in women with polycystic ovary syndrome (PCOS).Methods Sixty PCOS-IR women were randomized into treatment group and control group,30 cases in each group.At the end of the trial,26 cases in the treatment group (4 cases dropped out) and 29 cases in the control group (one case dropped out) completed the trial.The treatment group was treated with berberine orally,and the control group was treated with metformin orally,the treatment for the two groups lasting 3 months.Before and after treatment,we observed the parameters of body mass index (BMI),homeostasis model of assessment for insulin resistance index (HOMA-IR),fasting plasma glucose (FPG),fasting insulin (FINS),2-hour postprandial glucose after oral glucose tolerance test (OGTT2hPG) and 2-hour postprandial insulin after OGTT (OGTT2hINS),blood lipid levels of total cholesterol (TC),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C),and sexual hormones of follicle-stimulating hormone (FSH),luteotropic hormone (LH),testosterone (T) and LH/FSH ratio.Results BMI,HOMA-IR,TC,TG and LDL-C of the two groups were decreased after treatment (P ＜ 0.05 or P ＜ 0.01 compared with those before treatment),but the differences between the two groups were insignificant(P ＞ 0.05).Serum levels of T and LH as well as LH/FSH ratio in the treatment group were also decreased (P ＜ 0.05 or P ＜ 0.01 compared with those before treatment and those in the control group after treatment).Conclusion Berberine can improve HOMA-IR,decrease serum sexual hormones levels,and regulate the blood metabolism in PCOS-IR women,and its effect is similar to that of metformin.

Objective To develop a computer-aided diagnosis(CAD)system with automatic contouring and morphologic and tex-tural analysis to aid on the classification of breast nodules on ultrasound images .Methods A modified Level Set method was pro-posed to automatically segment the breast nodules(46 malignant and 60 benign nodules) .Following ,16 morphologic features and 17 texture features from the extracted contour were calculated and principal component analysis(PCA)was applied to find the optimal feature vector dimensions .Fuzzy C-means classifier was utilized to identify the breast nodule as benign or malignant with selected principal vectors .Results The performance of morphologic features was 78 .30% for accuracy ,67 .39% for sensitivity and 86 .67%for specificity ,while the latter was 72 .64% ,58 .70% and 83 .33% ,respectively .After the combination of the two features ,the re-sult was exactly the same with the morphologic performance .Conclusion This system performs well in classifying the malignant breast nodule from the benign breast nodule .