Objective To investigate the sleep quality of teachers from preschool institution and related factors.Methods Totally 220 teachers of various preschool institution in Shandong and Beijing were selected by cluster sampling and surveyed with questionnaire including Pittsburgh sleep quality index (PSQI).Results ①There were 27.7％ of the teachers having sleeping problems.②There was statistical significance by sleep disorder,using of sleep medications,daytime dysfunction,the PSQI scores of groups were significantly different (P ＜0.05).③Age of 36 ～ 40 years old (2.00 ± 0.71),married teachers (1.57 ± 0.72),seniority in 36 ～ 40 years,length of service in 13-17 years (1.75 ± 1.03) and ＞ 18 years (1.76 ± 0.66),form teacher(1.50 ± 0.71),formal staff teachers(1.63 ± 0.71)in sleep disorder were significantly higher than other teachers in their group and the difference was significant (P ＜ 0.05).College degree (0.22 ± 0.68),private kindergarden teachers (0.65 ± 0.90)in using of sleep medications were significantly higher than other teachers in their group,the difference was significant (P ＜ 0.05).formal staff teachers (1.33 ± 0.77) in daytime dysfunction were significantly higher than other teachers in their group and the difference was significant(P＜ 0.05).@The multiple factor logistic regression analysis demonstrated that sleeping quality of preschool institution ’s teachers were associated with having breakfast,regular diet,work breaks,insist on physical excise,Job burnout.Conclusion The sleep disorder is serious among teachers of preschool institution.