Gwe, an active ingridient derived from Chinese herb, possess profound effects on immune functions in mice in vivo and human peripheral lymphocytes (PBL)in vitro. The mitogenesis stim ulated with ConA or LPS, LAK cytotoxicity, spontaneous proliferation of thymocytes and prodution of IL-2 may all be increased markedly after treatment with Gwe in mice, but the CIC (circulating immune complexes) in sera decreased conpared to saline controls. The results shows that GWe influence preferentially on T-cell in a dose-dependent manner.

Objective:To explore the effect of jiudaiqingyuan oral solution in the treatment of chronic hepatitis-B(CHB) and in the elimination of HBV when used alone and combined with lamivudine.Methods:108 CHB patients were randomly divided into three groups:treatment group with Jiudaiqingyuan Oral Solution (Jiudai group),Lamivudine treatment group (lamivudine group),and treatment group with the combination of Jiudaiqingyuan Oral Solution and lamivudine (combination group),with each group including 36 cases.Laboratory tests were done before treatment,3,6,9 and 12 months after treatment,and at the 3rd,6th and 12th month of follow-up.The laboratory tests consisted of ALT,HBV-M and HBV-DNA.Results:At the 12th month of treatment and 12th month of follow-up,cases with normal ALT conversion cases with negative HBV-DNA conversion,cases with negative HBeAg conversion,HBeAg/anti-HBe serum conversion rate,and total effective rate of the combination group were respectively 94.4%,86.1%,55.6%,52.8%,75.0% and 83.3%,72.2%,47.2%,47.2%,72.2%,which were markedly better than those of Jiudai group or lamivudine group,with significant statistc difference in each index (P

In this paper, it has been found that such stressors as operation or swimming in cold watermay suppress the mitogenic response of murine spleen cells to ConA or LPS, whichis closely related to the action of suppressor cells.Using the plastic-adherence method, the plastic-adherence cells in spleen(PAC)was removed or obtained. The difference of mitogenic responsivebefore and after the removal of PAC is very apparent in stressed mice, being much lower thannormal before the renioval of PAC and near to normal after removing PAC. It is interestingthat the lymphocyte transformation stimulated by LPS is suppressed predominantly after swim-ming stress instead of those by ConA in operation stress.It has also been observed that theindomethacin may block the induction of suppressor PAC by stress, which means that the pro-staglandins may play an important role in the induction and action of suppressor PAC.

A total of 184 teeth obtained from 102 cases(27 males and 75 females aged 17-64 years)were selected from Department of Tooth and Dental Pulp.DalJan Stomatology Hospital from June to Septembet 2007.All the teeth with deep caries which resulted from caries showed no pulp.Ninety-two teeth obtained from 54 cases were regarded as Vitrebond group which were used by light-cured glass to cover pulp;while,the other 92 teeth obtained from 48 cases were regarded as dycal group which were cured by dycal calcium hydroxide.Finally,3MZ250 resin was used in this study.A half-year following up showed that the filling shed and loose were observed in the Vitrebond group(n=1)and the dycal group(n=3)(P>0.05).There were significant differenoe in color between Vitrebond group(n=1)and dycal group(n=8)(P<0.05),suggesting 3M Vitrebond light-cured glass Was superior to dycal calcium hydroxide in decreasing dental pulp stimulation and improving dental appearance.

0.05). The laser group with the dose of 3 J?cm-2 showed more increase in cell survival at 24 and 48 h after being seeded as compared with control group(P

DICOM medical data may be blocked by firewalls between terminals when DICOM3.0 Standard is applied in transmission via Internet. Since most of the firewalls are opened for emails, this paper introduces a new method that uses both email and DICOM3.0 protocols to transmit medical images via Internet.

Gynecologic personnel training is an important part of traditional Chinese medicine(TCM) of qualified personnel in the new century. With the opportunities and challenges in these years, improving the quality of TCM Gynecologic personnel training to adapt to economic and social development has great significance. For the situation of Gynecologic students difficult to word, and poor ability to adapt clinical reasons, we propose a five complex combination of moral, intellectual, research, economics, thinking education model, training Gynecologic compound talents, educating more skilled talents for medical practice.

BACKGROUND:It is suggested that glucose metabolism of frontal lobes,cingulate gyri and caudate nuclei in cerebral functional area of patients with depression is low,and the blood flow in cerebellum decreases.OBJECTIVE:To observe the effect of scalp acupuncture on cerebral glucose metabolism in depression by positron emission tomography.DESIGN:Randomly controlled and verifying experiment.SETTING:Department of Acupuncture and Aoxibustion in Nanfang Hospital and Center of Positron Emission Tomography in a Guangdong People's hospital.PARTICIPANTS:Twelve patients of primary depression,from Nanfang Hospital,Zhujiang Hospital and Center of Positron Emission Tomography in Guangdong People's Hospital from October 2002 to October 2003,were selected randomly with the diagnostic criteria of [F32] in the Third Edition of the Chinese Classification and Diagnostic Criteria of Mental Disease.Among them,3 were male and 9 were female.Their ages were from 36 to 66 years,with the average of 48.METHODS:Giving scalp acupuncture in middle line of vertex(MS5),middle line of forehead(MS1) and bilateral lateral line 1 of forehead(MS2).All the patients received positron emission tomography examination before and after acupuncture treatment for 6 weeks.Then cerebral Regions of Interest,bilateral frontal lobes,bilateral caudate nuclei,bilateral cingulate gyri and bilateral cerebella,were chosen and their average glucose metabolisms were counted by half-quantifying analysis.MAIN OUTCOME MEASURES:Glucose metabolism of bilateral frontal lobes,bilateral caudate nuclei,bilateral cingulate gyri and bilateral cerebella.RESULTS:According to intension,all 12 patients with primary depression sults of right frontal lobes(2.91 ±0.34),left frontal lobes(2.96±0.26),right cingulate gyri(2.68±0.20),right caudate nuclei (2.61±0.39) and left cerebellum(2.55±0.18)were obviously higher than those of right frontal lobes(2.53±0.31),left frontal lobes(2.53±0.36),right cingulate gyri(2.34±0.31),right caudate nuclei(2.40±0.33) and left cerebellum(2.35±0.25)before treatment(P＜ 0.05-0.01).However, the results of left cingulate gyri (2.69 ±0.38),left caudate nuclei(2.66±0.01) and right cerebellum(2.50±0.27) were similar to those of left cingulate gyri(2.43±0.38),left caudate nuclei(2.45±0.21) and right cerebellum(2.30±0.34) before treatment(P ＞ 0.05).CONCLUSION:Scalp acupuncture on middle line of vertex,middle line of forehead and bilateral lateral line 1 of forehead could improve the glucose metabolism in depressive patients of certain cerebral regions,and it might be a mechanism related to improvement of frontal lobes,caudate nuclei,cingulate gyri and cerebellum.

BACKGROUND:Menstrual blood-derived mesenchymal stem cels are proved to be a new type of adult stem cels. To date, little is reported on the methods of establishing this kind of cel lines systematicaly.
OBJECTIVE:To isolate, culture, identify and finaly establish menstrual blood-derived mesenchymal stem cel lines.
METHODS:Menstrual blood-derived mesenchymal stem cels were isolated from the menstrual blood of healthy female donors and cultured accordingly using density gradient centrifugation method. Cel morphology and proliferation characteristics were observed, and proliferative ability was detected by cel counting kit-8 assay. Cels were colected periodicaly for karyotype analysis. Expressions of cel surface antigens such as CD34, CD38, CD44, CD45, CD73, CD90, CD105 and Oct-4 were analyzed using flow cytometry. Cel pluripotency were identified by inducing adipogenesis and osteogenesis in vitro.
RESULTS AND CONCLUSION:We successfuly established four menstrual blood-derived mesenchymal stem cel lines from the menstrual blood of six healthy volunteers. The cultured cel lines had a typical spindle shape and kept normal 46, XX karyotype. The cels grew rapidly and entered into the logarithmic growth phase at 48 hours after passaging. The results of flow cytometry showed that menstrual blood-derived mesenchymal stem cels were positive for CD44, CD73, CD90, CD105 and Oct-4, while negative for CD38, CD34 and CD45. Menstrual blood-derived mesenchymal stem cels were positive for oil red O staining at 22 days after adipogenic induction and also positive for alizarin red staining at 2 weeks after osteogenetic induction. In conclusion, our study suggests that menstrual blood-derived mesenchymal stem cels with stable karyotype and pluripotency can proliferate quickly, and we can provide a new resource for stem cel therapy and regenerative medicine through establishing the menstrual blood-derived mesenchymal stem cel lines.