Objective To study the clinical significance of changes of blood contents of vascular endothelial growth factors (VEGF).Pregnancy-associated plasma protein-A(PAPP-A),D-dimers(D-D)and Urinary N-acetyl-β-D-glucosaminidase/Creatinine (NAG/Cr)Ratio in patients with Pregnancy Induced Hypertension syndrome(PIH).Methods Serum VEGF and PAPP-A(with ELISA),plasma D-dimers(with biochemistry)and NAG/Cr(with velocity)contents were measured in 92 pregnant women with PIH and 35 controls.Results The serum VEGF contents in patients with PIH were significantly lower than those in controls(P<0.01)while PAPP-A,D-dimers and NAG/Cr contents were significantly higher(P<0.01).Those changes were especially marked in patients with advanced diseases.Conclusion Changes of blood contents of VEGF,PAPP-A D-dimers and Urinary NAG/Cr Ratio were closely related with the disease process of PIH.Determination of these changes was of clinical diagnostic as well as prognostic value.

Objective To explore the clinical value of tumor marker squamous cell carcinoma antigen(SCCA) ,tissue polypeptide specific antigen(TPS) ,neuron‐specific enolase(NSE) ,cytokeratin fragment 19(CYFRA21‐1) detections in the patient with lung cancer .Methods 70 patient with newly diagnosed lung cancer(including 26 cases of squamous cell carcinoma ,23 cases of adenocar‐cinoma and 21 cases of small cell carcinoma) ,40 cases of lung benign diseases and contemporaneous 30 individuals undergoing the physical examination were randomly selected as the lung cancer group ,lung benign disease group and healthy control group respec‐tively .Serum tumor markers of SCCA ,TPS ,NSE and CYFERA21‐1 were detected in all cases .Then the detection results were per‐formed the statistical analysis .Results The levels of SCCA ,TPS ,NSE and CYFERA21‐1 in the lung cancer group were significant‐ly higher than those in the lung benign diseases group and healthy control group ,the differences were statistically significant (P<0 .05) .The expression levels of serum SCCA ,TPS ,NSE and CYFERA21‐1 were different in different histological types of lung cancer .SCCA and CYFERA21‐1 were highly expressed in squamous cell carcinoma ,which showed the statistical difference com‐pared with other two pathological types of lung cancer(P<0 .05);the expression level of NSE was highest in small cell lung cancer , which showed the statistical difference compared with other two pathological types of lung cancer(PP<0 .05);the TPS expression level had no statistical difference among different pathological types of lung cancer(P>0 .05) .The combination detection of SCCA , TPS ,NSE and CYFERA21‐1 greatly increased the sensitivity and accuracy for diagnosing lung cancer .Conclusion The combina‐tion detection of SCCA ,TPS ,NSE and CYFERA21‐1 has very high clinical value for the diagnosis ,pathological typing and assisted clinical therapy of lung cancer .

Objective To observe the clinical effect of electroacupuncture and Shenmai injection combined with conventional western therapy as treatments for the sudden deafness.Methods A total of 186 patients with sudden deafness were randomly divided into two groups. Each group included 93 patients. The control group was treated with the pipe-expanding and anti-inflammatory, but the treatment group was treated with electroacupuncture and Shenmai injection based on the control group. Both groups were treated for 11 days.Before and after treatments, the regional cerebral blood flow (rCBF) was detected. The MADSEN was used to detect ocular vestibular evoked myogenic potential (oVEMP), including N1-Pl amplitude, N 1-Pl incubation period, N1-Pl wave duration and extraction rate of oVEMP.Results The recovery rate of control group was 63.4% (59/93) and total effective rate was 90.3% (84/93), which was 88.6% (75/93) and 97.8% (91/93) in combined treatment group, and there was significant difference between the 2 groups (χ2=5.923,P<0.05). After 11 days of treatment, the Tinnitus (17.2%vs. 30.1%,χ2=7.152), vertigo and survival rate (15.1%vs. 21.5%, χ2=6.023) in combined treatment group showed significantly lower than those in the control group (P<0.05). The threshold (39.59 ± 5.36 dBHLvs. 45.85 ± 5.08 dBHL,t=2.903) in combined treatment group showed significantly lower than those in the control group (P=0.034). The N1 amplitude (10.62 ± 0.84μVvs. 7.14 ± 0.59μV;t=3.259,P=0.017), P1 amplitude (11.79 ± 0.91μVvs. 9.90 ± 0.82μV;t=2.871,P=0.037), extraction rate of oVEMP (95.7%vs. 81.7%;χ2=7.963,P=0.012) in combined treatment group showed significantly higher than those in the control group. The N1 incubation period (7.86 ± 0.82 msvs. 9.78 ± 1.24 ms;t=3.729,P=0.009) and Pl incubation period (6.57 ± 0.77 msvs. 9.39 ± 1.15 ms;t=3.064,P=0.025) in combined treatment group showed significantly lower than those in the control group.Conclusions The Electroacupuncture and Shenmai injection combined with conventional western therapy could improve blood circulation produce a synergistic therapeutic effect on damaged tissue, improve cochlear hair cells and vestibular nerve regeneration, and repaire the functions.

Progesteron could significantly inhibit the contracted effect of strips of tracheal and lung smooth muscles isolated from guinea pigs, which induced by Ach His and 5- HT. It also inhibited the contractility of phase I caused by intracellular calciumefflux in the course of 5 -HT stimulating tracheal smooth muscles and the Schultz - Dale responsiveness of sensilized of tracheal smooth muscles guinea pigs.

To investigate the immunophenotypings of malignant epithelial mesothelioma (MEM), and to seek the valuable markers in distinguishing peritoneal MEM from peritoneal metastatic ovarian adenocarcinoma (OA) and colorectal adenocarcinoma (CA), immunohistochemical SP method was used to detect expressions of HBME-1, E-cadherin, CA19-9, MOC-31 and CK7 in paraffin-embedded tissues of 18 cases of MEM, 20 OA and 20 CA. The results showed that there was a significant difference in the expressions of E-cadherin, CA19-9 and MOC-31 between MEM and OA group (P<0.05). Similarly, the difference in the expression of HBME-1, E-cadherin, CA19-9, MOC-31 and CK7 between MEM and CA groups is significant (P<0.05). These results indicate that HBME-1 could be used as a positive marker in distinguishing MEM from CA. E-cadherin, CA19-9 and MOC-31 are considered to be useful negative markers in diagnostic distinction between MEM and metastatic adenocarcinomas, including OA and CA. CK7 is the best positive marker in distinguishing MEM from CA, but this marker appears to be valueless in discriminating MEM from OA.

In order to explore the value of p63, smoothmuscle actin (alpha-SMA) and cytokeratin 5/6 (CK5/6) in the differential diagnosis of ductal lesions of breast, 88 tissue specimens of ductal lesions of breast were collected and examined histologically by HE staining. By using immunohistochemistry, the expression of p63, alpha-SMA and CK5/6 was detected. The results showed that in 38 cases of benign breast lesions, the proliferating cells were all positive for p63 and alpha-SMA. In 19 cases of ductal carcinoma in situ (DCIS) and 7 cases of intraductal papillary carcinoma, alpha-SMA positive cells formed a layer of continuous embroider-shaped structure and the p63 positive cells formed a layer of evenly separated embroider-shaped structure around the ducts. There was no cross-reaction between p63 and interstitial myofibroblasts and vascular smooth muscle cells. In 38 cases of benign breast lesions, the positive rate of CK5/6 expression was 100%. In 5 cases of atypical ductal hyperplasia, there were few positive cells in the ducts. In 19 cases of CDIS, no tumor cells expressed CK5/6. In 19 cases of invasive ductal carcinoma, almost no CK5/6 was detectable. It was suggested that p63 could serve as a novel specific marker for the identification of breast myoepithelial cells. CK5/6 is of value in differentiating ductal proliferation of varying degrees, especially in the differentiation between cancerous and non-cancerous changes. Simultaneous detection of p63, CK5/6 and alpha-SMA can help increase the diagnostic accuracy of breast diseases.

Objective To explore the clinical value of colour doppler flow imaging (CDFI) in selecting the ectopic pregnancy(EP) suitable for medical treatment. Methods 50 cases of ectopic pregnancy with hematodynamic stability were injected with a single dose of 50mg/m 2 MTX after CDFI examination. All patients were followed up until clinical results were confirmed. The CDFI characteristics of the failure and the success cases were analysed in combination with their serum HCG levels, and a CDFI-based pretherapeutic score were designed. Results CDFI could reflect the viability of ectopic fetus directly.There was a significant difference in the successful rate of medical treatment of ectopic pregnancy among the patients with various types of CDFI images and the various levels of serum HCG (P10 had the high failure rate, thus medical treatment of ectopic pregnancy is not recommended to them.

To determine the effects of testosterone (T) on hypertrophy and cardiac function of rats with myocardial infarction (MI), the rats were divided into 3 groups: ie, the sham-operated group (S group), the coronary artery ligating groups with or without the injection of T (MIT and MI group). T level of plasma was tested on all rats, by RIA on1/4, 1, 3, 9, 20 day after operation; then the nuclear profiles per unit area of myocardium N_((n)A) the average nuclear length D_((n)) and the volume fraction of number of myoeytes in the myoeardium V_((m)v) were measured under microscope; The number of myocyte nuclei per unit ovolume of myocardium N_((n)v) and average myocyte cell volume per nucleus V_((m)n) were calculated. The results were as follows: (1) Comparing with S group N_((n)A), N_((n)v) and V_((m)n) in MI had marked changes (P0.05). Of all the mentioned changes were more marked in MIT group than that in MI group. (2) The total plasma concentration of T was correlated with V_((m)n) (r=0.60, P0.05) were not found in MIT rats. These results indicated that T may improve myocardial hypertrophy and promote the recovery of eardial function of MI.

The fine composition of volatile oils in a raw peppermint in July were analysed by combined means of programmed temperature capillary gas chromatography/mass spectrometry (GC/MS).20 compounds were separated and identified.The main constituents were (1α,2α,5β)-menthol (16.88%),(1α,2β,5β)-menthol (74.98%),cis-menthone(3.84%) and (2R-cis)-menthone (1.25%).In the whole July mint there were 4 groups of isomeric compound,their molecular formula were C10 H16 (5 components),C10 H18 O (2 components),C10 H20 O (2 components) and C15H24(8 components),respectively.

Objective: To explore and modify the isolation method for mouse intestinal intraepithelial lymphocytes. Methods: Epithelium mucosae of mouse small intestine was incubated in iced bath and shaked in PBS containing DTT. The cell suspension was obtained after filtration with 80 and 400- screen mesh trap valve in turn. The yield, viability and purity of intestinal intraepithelial lymphocytes were observed to estimate the feasibility. Results: About (5.6±0.7)×10～6 intestinal intraepithelial lymphocytes were obtained from every 20cm samll intestine. The viabilty of intestinal intraepithelial lymphocytes was (90.46±5.71)% and the purity was (92.21±5.20)%. Conclusion: Compared with other reported isolation methods, the modifled method is convenient and esay to handling.The yield, viability and purity are high enough to be used for intestinal intraepithelial lymphocytes studies.