Multi-modality imaging system based on fluorescence molecular tomography (FMT) has been widely used in animal studies.It combines FMT with other imaging modalities,which realizes the imaging of anatomical structure,physiological function and biological activities on molecular or cellular levels of small animal in vivo at the same time.This review gives an introduction to the history and current research status of singlemodality FMT system,introduces the developments of multi-modality system based on FMT,especially the system setup,working principle,performance and application of FMT/CT,FMT/MRI and FMT/radionuclide imaging.The prospects of multi-modality imaging system based on FMT are discussed.