Objective To examine serum TFAR19 activity in uterine myoma patients.Methods 45 of operation patients with uterine myoma in Beijing Huairou Maternal and Child Health Hospital were recruited.Among them,diameter of maximal myoma from 6 to 10 centineters were 29 (group A),diameter of maximal myoma more than 10 centineters were 16 (group B).In addition,25 unrelated healthy women of childbearing age from physical examination center were enrolled as controls (group C),all of the peripheral venous blood was collected after admission.Enzyme-linked immunosorbent assay (ELISA) was used to examine serum TFAR1 9 activity in uterine myoma patients.Results The activity of TFAR1 9 in group A,group B and group C were 25.32±1.93,14.41±2.28 and 34.79±4.65 respectively.The TFAR19 activity in each group was sta-tistically significant difference (F=7.93,P=0.01).Compared with group C,the TFAR19 activity in group B (q=5.606,P<0.001),group A (q=3.055,P=0.034)was statistically significant difference.The TFAR19 activity in uterine myoma patients subgroups (group A,group B)was statistically significant difference (q=3.086,P=0.033).Age between each groups was not statistically significant difference (F=2.086,P=0.132).Conclusion The decrease of TFAR19 can inhibit apoptosis.Participating the occurrence of uterine myoma,it is connected with diameter of myoma.The larger diameter of my-oma,the lower TFAR1 9 activity of examination will be.

OBJECTIVE To investigate the distribution and drug resistance of clinical Pseudomonas aeruginosa (isolates),and offer reasonable experimental data for clinical therapy.METHODS A statistic analysis for the(specimen) source,departments with infection and drug resistance condition to 218 isolates was conducted in(retrospective) manner.RESULTS Among 218 strains 138(63.3%) come from sputum and throat mucus,and 25(11.5%) from wound swab;the proportion of these strains from ICU was 44.9%,from neurology department was 12.4% and from(respiratory) department was 11.5%.The result of drug susceptibility showed that P.aeruginosa had a high resistance rate to the ?-lactamases, aminoglycosides,fluoroquinolones and sulfanilamides(antibiotics),respectively,accounted for more than 50%,and had a lower resistance rate to carbapenem, ?-(lactamases) antibiotics/inhibitor,the lowest was to piperacillin/tazobactam(8.7%),and to cefoperazone/(sulbactam)(17.5%).CONCLUSIONS Clinical(isolates) of P.aeruginosa come from the sputum,(pharyngeal) swab and ICU mainly,but their resistant rates are rather high and multidrug-resistant.We should pay more attention to the surveillance of antimicrobial resistance of P. aeruginosa in clinics and prevent(transmission) and epidemic of their resistance strains.

Objective To explore serum TFAR19 activity in preeclampsia patients.Methods 43 of cesarean delivery patients with preeclampsia in the Huairou Maternal and Child Health Hospital of Beijing were recruited and the peripheral venous blood was collected.Among them,severe preeclampsia patients were 18,mild preeclampsia were patients 25.In addition,40 unrelated healthy cesarean puerpera from a routine health survey were enrolled as controls.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum TFAR19 activity in preeclampsia patients.That was absorbancy-A catalytic genera-ted by TFAR19.Results The activity of TFAR19 severe preeclampsia patients,mild preeclampsia patients,and healthy con-trols were 43.56±2.93,33.38±2.32 and 23.63±2.94,respectively.The TFAR19 activity in each group was statistically significant difference (F = 10.65,P <0.01).Compared with healthy controls,the TFAR19 activity in severe preeclampsia group (q=6.384,P <0.001),mild preeclampsia group (q = 3.478,P =0.016)was statistically significant difference.The TFAR19 activity in preeclampsia patients subgroups (severe preeclampsia group,mild preeclampsia group)was statistically significant difference (q = 2.993,P = 0.037).Age between each groups was no-statistically significant difference (F =0.091,P =0.913).Gestation age and neonatal weight betweeneach groups was statistically significant difference (F=4.37, P =0.016;F=35.06,P <0.001).Conclusion The increase of TFAR19 could promote apoptosis,participating the occur-rence of preeclampsia.The content of TFAR19 could be increased with the severity of the preeclampsia.

Objective To study the distribution and drug resistance of clinical Providencia stuartii isolates and provide the basis data for clinical therapy.Methods A data analysis for the specimen source,the distribution of departments with infection and the antibiotic susceptibility results to 76 isolates was conducted in retrospectively. Results Among 76 strains were mainly from sputum (71.1%),wound secretions (10.5%) and blood (6.6%);the proportion of these strains from ICU was 40.8%,from respiratory department was 9.2%and from general surgery was 7.9%;The bacteria's drug resistance was more serious,multi drug resistant (MDR) strains accounted for 65.8%,pan drug resistant ( PDR) strains accounted for 26.3%,the mortality of patients with PDR strains infection was 45.0%,which was higher than 5.8%of no PDR strains infection.A low resistance rate of antibiotics were imipenem,fluoro-quinolones andamikacin,et al,and the rate of imipenem resistant was lowest(31.5%).Conclusion The multidrug resistance phenomenon of Providencia stuartii is serious, the first choice for the treatment is imipenem, the second choice was fluoroquinolones or amino glycopeptides antibiotics,the hospital infection caused by the bacterium infection in monitoring should be strengthened.

Objective To investigate the possibility of early diagnosis of colorectal cancer by combined detec-tion of tumor markers dermcidin(DCD),CA199,CA724 and CEA. Methods We selected 81 colorectal cancer patients and 30 healthy individuals. Serum levels of DCD were detected by ELISA,and serum levels of CA199, CA724 and CEA were detected by electrochemical luminescent immunoassay,and the expression of DCD in colorectal cancer tissue and para-carcinoma tissue was measured by Western blot. Results Serum levels of CA199,CA724,and CEA were significantly higher in colorectal cancer patients than in healthy individuals(P<0.001). Combined detection of DCD,CA199,CA724 and CEA had a greater AUC(0.942)for identifying colorec-tal cancer from the controls. The expression of DCD was significantly higher in colorectal tissue than in para-carci-noma tissue(P<0.05). Conclusions Level of DCD expression was significantly higher in colorectal tissue than in para-carcinoma tissue. Expressions of serum DCD,CA199,CA724,and CEA may be a marker in diagnosis of can-cer,with higher clinical value for the adjuvant diagnosis of colorectal cancer.