OBJECTIVE:To study the pharmacokinetics of a single dose of intramuscularly injected ziprasidone mesylate in healthy volunteers.METHODS:A total of10healthy volunteers were received intramuscularly single dose of ziprasidone me-sylate20mg,venous blood samples were taken at different time after intramuscular injection,the concentrations of ziprasidone in serum were determined by HPLC,and the pharmacokinetic parameters were calculated with3p97program.RESULTS:The concentration-time curves of ziprasidone fitted to two-compartment model.The pharmacokinetic parameters were stated as follows,C max was(866.32?482.88)ng/ml;t max was(0.57?0.23)h,t 1/2ke was(2.17?1.04)h,CL was(17.0?9.00)ml/h,AUC 0～12 was(1467.94?644.80)(ng?h)/ml and ACU 0～∝ was(1603.48?722.47)(ng?h)/ml.CONCLUSIONS:The pharmacoki-netics parameters in10healthy volunteers after intramuscular injection of20mg ziprasidone mesylate are in line with that re-ported in the literature.

Objective To observe the influence of T cell subsets and natural killer cell ofcytokine induced killer (CIK) therapy for partial advanced non-small cell lung cancer patients.Methods Sixty-one patients with partial advanced non-small cell lung cancer were randomly divided into treatment group and control group by sortition method.In treatment group,31 patients received three dimensional conformal radiation therapy and chemotherapy,then received CIK treatment.In control group,30 patients received three dimensional conformal radiation therapy and chemotherapy.Results In treatment group,CD3+,CD4+,CD4+/CD8+ and CD16+ CD56+ after treatment were significantly higher than before treatment (0.7125 ± 0.0154 vs.0.6374 ± 0.0325,0.3921 ± 0.0461 vs.0.3217 ± 0.0287,0.0178 ± 0.0179 vs.0.0102 ± 0.0093,0.1855 ± 0.0321 vs.0.1224 ± 0.0177),CD8+ after treatment was significantly lower than before treatment (0.2209 ± 0.0257 vs.0.3147 ± 0.0307),there was significant difference (P ＜ 0.05).In control group,there were no significant difference in CD3+,CD4+,CD8+,CD4+/CD8+ and CD16+ CD56+ between after treatment and before treatment (0.6218 ± 0.0040 vs.0.6325 ± 0.0114,0.3311 ± 0.0278 vs.0.3347 ± 0.0209,0.3265 ± 0.0257 vs.0.3316 ± 0.0178,0.0101 ± 0.0108 vs.0.0101 ± 0.0117,0.1327 ± 0.0298 vs.0.1479 ± 0.0354,P ＞ 0.05).The side effects in the treatment group and control group showed no significant difference (P ＞0.05).Conclusion CIK therapy can improve immune function in patients with partial advanced non-small cell lung cancer.

0 05) , repectively. Of all dementia, AD and VD accounted for 58 48% and 31 58%, respectively. The AD prevalence in female was higher than that in male( P 0 05). The prevalence of total dementia, AD and VD tended to increase with aging( P 0 05). The prevalence of dementia increased one fold every five year of age increasing. The prevalence of dementia and AD in illiterate group was higher than in educated people with elementary school level and above junior high school level ( P

DS ③Combined MMSE with one or more of FOM、RVR 、BD、DS,the validity of diagnosis was improved.Conclusion: Neuropsychological test is useful in diagnosis of AD.The validity is improved when MMSE are combined correctly with other rating scales.

Objective To investigate the efficacy and toxicity of etoposide combined with prednisone (EtoP regimen) for elderly patients with unspecific peripheral T-cell lymphoma (PTCL-U) compared with the CHOP regimen.Methods 23 elderly PTCL-U patients were randomly assigned to EtoP group (n =12) or CHOP group (n =11).The curative effects and the adverse reactions were analyzed and compared between the two groups by Log-rank test and x2 test.Results The overall response (OR) rate was 66.67 ％ (8/12) in EtoP group and 63.64 ％ (7/11) in CHOP group.There was no statistically significant difference between the two groups(x2 =0.023,P =0.879).The progression-free survival (PFS) was 7.55 months in EtoP group and 4.38 months in CHOP group,statistically significant difference were observed between the two groups (x2 =23.000,P =0.011).The overall survival (OS) was 15.02 months in EtoP group and 12.26 months in CHOP group,no statistically significant difference was observed between the two groups (x 2 =14.985,P =0.597).The main side effects were bone marrow depression and digestive tract toxicity,no hematology toxicity and digestive tract toxicity of grade Ⅲ-Ⅳ occurred in EtoP group.Of those in CHOP group were 36.36 ％ and 27.27 ％.Conclusion For elderly PTCL-U patients,EtoP regimen is an effective and well tolerated therapeutic schedule.

0.05). Estrogen was lower in the patients with AD than in the normal controls and there was a significant difference between the two groups (P

Objective To analyse the levels of estrogen in women patients with Alzheimer's disease(AD),and the relationship of estrogen level,menarche age,age of menopause,pregnancy times with severity of AD.Methods A 1:1 matched case-control study was conducted.The study subjects were given neuropsychological test face to face in questionnaire,including MMSE,ADL,POD,FOM,WISE and HMT depression scale.All the women subjects accepted another questionnaire concerning menarche age,age of menopause and pregnancy times.Radioimmunoassay was used to measure the sex hormone level of all the subjects.Results The level of estrogen in serum was obviously lower in AD group than in the control group(P

Objective To investigate the abnormalities of brain gray matter volume in patients with clinically-confirmed essential tremor (ET)of the hands only.Methods We analyzed brain gray matter voxel of 1 7 patients (younger than 55 years)with ET of the hands only and 1 7 healthy controls matched in age,gender and education by optimized voxel-based morphometry (VBM).Results VBM showed marked expansion of the bilateral cerebella, occipital fusiform cortices,and precentral lobes (P uncorrected < 0.005 )in ET patients compared with the controls. Atrophy was only detected in left parietal lobe.We also found volume enlargement in the thalamus,midbrain,and melluda of the left side by region of interest (ROI )analysis (P uncorrected <0.005).Conclusion Patients with arm tremor show expansion of gray matter,which might represent the adaptive reorganizational compensation through the increased demand on the visuospatial control of skilled movements in ET patients with early-stage arm tremor. These morphological changes may help to assess early stage and distinguish subtype of ET.

Objective To investigate the apoptosis-inducing effect of L-gossypol on human nasopharyngesl carcinoma cell line CNE2 and its possible mechanism.Methods The effect of L-gossypol on proliferation of CNE2 cells was estimated by MTT assay.Flow cytometry was used to analyz cell apoptosis induced by(-)-gossypol and the expression of Bcl-2 、Bax proteins.Caspase-3 activity was determined by caspase-3 colorimetric assay kit.Results Lgossypol was able to inhibit the proliferation of CNE2 cells in a dose-dependent and time-dependent fashion at concentrations more than 10 μmoL/L.L-gossypol regulated cell cycle and GO/G1 arrest could be observed in CNE2 cells.In the process of apoptosis,the expression of Bcl-2 was down-regulated and Bax was up-regulated,caspase-3 activity was in peak at 24h.Conclusion L-gossypol could induce apoptosis in nasopharyngeal carcinoma cell line CNE2 in vitro,which may be associated with down-regulation of Bcl-2,up-regulation of Bax genes expression and caspase-3 activation.

OBJECTIVETo learn the existence of natural focus of Lyme disease and its distribution.

METHODSA semi-nested polymerase chain reaction (PCR) method was developed for detection and genotyping of Borrelia burgdorferi on basis of outer surface protein A (OspA) gene. Ticks and mice collected from 6 forest areas in Beijing were detected with above methods. The positive PCR products were cloned and sequenced. The sequences were compared with published sequences for homology. IFA as used to detect IgG antibody on Borrelia burgdorferi. Lyme disease spirochete were isolated from H. longicornis were also attempted.

RESULTSB. Burgdorferi sensu lato were detected from 939 ticks and 250 mice specimens collected from above 6 study sites using primer pairs OA(1)/OA(4) and SL/OA(4). Only the specimens collected from Dongling mountain showed positive amplification. One in three adult Ixodes persulcatus with one of 57 nymph Ixodes persulcatus showed positive while 9 of 119 (7.56%) mice specimens showed positive, of which 8 were B. grinii and one B. afzelii. In this study, we attempted to isolate B. burgdorferi sensu lato strains from 160 H. longicornis ticks (20/group) but failed. Serological survey showed a 9.1% (5/55) infection rate with B. burgdorferi sensu lato in the mice of Dongling mountain forest areas.

CONCLUSIONSThe natural focus of Lyme disease including B. garinii and B. afzelii might have existed in Dongling mountain of Mentougou district, Beijing. Ixodes persulcatu and mice may serve as vectors and reservoirs, respectively.

Animals ; Antigens, Surface ; genetics ; Bacterial Outer Membrane Proteins ; genetics ; Bacterial Vaccines ; Borrelia burgdorferi ; genetics ; Borrelia burgdorferi Group ; classification ; genetics ; Humans ; Ixodes ; microbiology ; Lipoproteins ; Lyme Disease ; microbiology ; Mice ; Phylogeny ; Polymerase Chain Reaction ; methods