0.05). Only the sVCAM-1 level of PILL type had significant difference with the healthy control group and KDDC type (P

Objective To study the effect of Mongolian medicine Senden decoction on IFN-γexpression of CIA model rats.Methods 10 rats were randomly selected as the normal group.The other 50 SD rats were established CIA model,and were randomly divided into 5 groups:the model group,Mongolian medicine morito decoction of high, medium and low dose group,tripterygium glycosides group.At different time points,the arthritis index,immunohisto-chemical expression of IFN-γwere detected in the synovial cytokine,the protein expression of IFN-γin jaw joint was detected by Western blot.Results Mongolian Senden decoction could improve CIA rat arthritis index,improve the IFN-γpositive expression in synovial joints, reduce the expression of IFN -γprotein in the claw gamma, tripterygium wilfordii group and Mongolian senden decoction high dose group improved the most obviously,and there was significant difference compared with model group(FAI 40d=27.276,FIFN-γPositive =32.654,FIFN-γprotein =674.124, all P=0.000).Conclusion Mongolian medicine Senden decoction can improve symptoms and IFN-γjoint inflam-matory cytokine expression in rat CIA model,the effect of joint synovial membrane,and the positive control medicine tripterygium glycosides, but there is a dose -response relationship, and low doses of Mongolian medicine senden decoction high dose effect is better than that of medium and low dose.

This article was aimed to analyze and summarize the historical origin, chemical constituents, pharmacological effects, clinical research and standardization research on Digeda-4 Flavored Decoction, in order to provide references for further study on Mongolian medicine and application. The historical origin, chemical constituents, pharmacological effects, clinical application and standardization research on Digeda-4 Flavored Decoction were analyzed and summarized through literature searching. It put forward the existing prescription problems and further research ideas. The results showed that Digeda-4 Flavored Decoction was a common Mongolian preparation. It had a long history with certain studies on its chemical constituents and pharmacological action of single herb. However, the research on compound prescription was still relatively backward. Studies on quality controlled standards, pharmacology, pharmacodynamics and toxicology studies were lacked of. The standardization, high speed, informationization were the future direction and trend of development. It was concluded that Digeda-4 Flavored Decoction was commonly used in Mongolian medicine. It was hoped to lay a solid foundation for its standardization research, pharmacological research, and standard clinical study in the future to develop Digeda-4 Flavored Decoction with stable quality, reliable curative effect, less toxic side effect, and convenient administration.

Objective To investigate the relationship between subclinical hypothyroidism (SCH) and diabetic retinopathy (DR) in patients with type 2 diabetes mellitus (T2DM).Methods A total of 792 patients of T2DM were enrolled in the study.There were 448 males and 344 females,with an average age of (54.13 ± 13.06)years.The average duration of diabetes was (8.03 4±6.70) years.The patients were grouped according to the degree of DR and thyroid function.Among them,483 patients (61.0％) were no DR,240 patients (30.3％) were mild DR,69 patients (8.7％) were severe DR.725 patients (91.5％) were normal thyroid function,67 patients (8.5％) were SCH.The prevalence of SCH among no DR group,mild DR group and severe DR group was compared.And the prevalence of DR between normal thyroid function group and SCH group was compared.Logistic regression analysis was used to estimate the association between SCH and DR.Results No significant differences among the three groups (no DR group,mild DR group,severe DR group) were found in the prevalence of SCH (x2=1.823,P=0.402).There were no significant differences in the incidences of DR between normal thyroid function group and SCH group (x2=1.618,P=0.239).Logistic regression analysis demonstrated that SCH was not significant associated with DR [mild DR:odds ratio (OR)=1.361,95％ confidence interval (CI)=0.773-2.399,P=0.286;severe DR:OR=1.326,95％CI=0.520-3.384,P=0.555;DR:OR=1.353,95％ CI=0.798-2.294,P=0.261).Conclusion SCH is not significant associated with DR in patients with T2DM.

OBJECTIVETo investigate the expression of orotate phosphoribosyltransferase (OPRT) in colorectal carcinoma and analyze its correlations with the toxicities of chemotherapy.

METHODSThe expression of OPRT mRNA was detected using RT-PCR in colorectal carcinoma tissues and paired adjacent normal tissues from 58 patients receiving FOLFOX6 regimen chemotherapy. The toxicities of the chemotherapy were recorded, and the correlations between OPRT mRNA expression and the toxicities were analyzed.

RESULTSOPRT mRNA expression was significantly higher in the tumor tissues than in the corresponding normal tissues (P=0.001), but OPRT expression in the tumor tissues was not correlated with the toxicities of the chemotherapy (P>0.05). OPRT level in the normal tissues showed a significant positive correlation with the occurrence of diarrhea in these cases (P=0.013).

CONCLUSIONOPRT expression in colorectal carcinoma tissues is not correlated with the toxicities of 5-FU-based regimen, but OPRT expression in the normal tissues can help predict the toxicities associated with 5-FU.

Adult ; Aged ; Antimetabolites, Antineoplastic ; therapeutic use ; toxicity ; Colorectal Neoplasms ; drug therapy ; metabolism ; pathology ; Female ; Fluorouracil ; therapeutic use ; toxicity ; Humans ; Intestinal Mucosa ; metabolism ; pathology ; Male ; Middle Aged ; Orotate Phosphoribosyltransferase ; metabolism

Objective To investigate the positive time length of nucleic acid detection in 22 confirmed cases of coronavirus disease (COVID-19) in Yangzhou and analyze the influencing factors, and to provide evidence for the prevention and control of the disease. Method A total of 22 confirmed cases were followed up for five weeks. Throat swabs were collected for nucleic acid detection. The relevant data were collected and statistical analysis was conducted on the basis of survival analysis. Results The positive rate of throat swabs was 100%, 100% and 66.67% at 1-2d, 3-4d and 5-7d, respectively, after the onset of COVID-19. The average positive time of all confirmed cases was 16.32 days, including 18.50 days for common type and 13.70 days for light type. The difference was statistically significant (χ²=4.36, P=0.037). Multivariate Cox regression analysis showed that case type (or=0.19, 95%CI:0.06-0.61) and onset visit time (or=0.70,95%CI:0.55-0.88) had an impact on the positive time length of nucleic acid detection. Conclusion The positive rate of respiratory samples is high within one week after the onset of the confirmed cases, and the positive time length of light type cases was shorter than that of common types. The positive time length of nucleic acid detection may be shortened after timely treatment with drugs.