Objective To identify the peak 2 protein on the right side of the main peak of size exclusion chromatograghy-high performance liquid chromatography(SEC-HPLC)in freeze-dried human rabies vaccine(Vero cell)stock solution(without human blood albumin),so as to determine the protein composition of the peak and ensure the vaccine quality.Methods The lyophilized human rabies vaccine(Vero cells)stock solution(without human blood albumin)was analyzed by SECHPLC,of which the peak 2 on the right side of the main peak was collected and concentrated by ultrafiltration,then reduced by dithiothreitol(DTT),alkylated by iodoacetamide (IAM) and hydrolyzed by Trypsin. The products were analyzed by nano LC-MS/MS.Results Four proteins were successfully identified in the right peak 2 of SEC-HPLC main peak in lyophilized human rabies vaccine(Vero cell)stock solution(without human blood albumin),and the number of matching peptides for a single protein ranged from 59 to 79. The number of single protein-matching characteristic peptides ranged from 6 to 12. The detection times of single protein-matching characteristic peptide segments ranged from 20 to 36 times. The sequence coverage of the identified proteins ranged from 37. 40% to 64. 31%. A total of 280 peptides participated in the statistics,and the mass spectrum deviation was less than 0. 15 Da.Conclusion The peak 2 on the right side of SEC-HPLC main peak of lyophilized human rabies vaccine(Vero cell)stock solution(without human blood albumin)is derived from rabies strain 4a GV,which is the vaccine particle dissociation.

Through the interpretation of traditional Honesty, this article is to understand the semantics of credibility andintegrity in traditional ethical culture, to analyze the relationship between the concept of honesty and medical skills, deriving goodness and endowing novelty, further to reveal the revelation for medical professional personality cultivation. Meanwhile, it points out that we should try our best to overcome the shortcomings of tradi-tional concept of honesty,clarify the connotation of honesty and find out the evidence of honesty culture in tradition-al Chinese ethics. This is the tracing and deepening of the connotation of honesty, as well as the highlighting of honesty as the content of medical professional personality. It is further beneficial to strengthen the pathway con-struction of medical profes.

Objective To study the status of surface plasmon resonance (SPR) detection of cervical human papillomavirus (HPV) infection in clinical practice.Methods 502 cases of cervical HPV in patients with cervical cell's genetic subtypes were diagnosed using SPR.Positive rates of HPV lesions of cervical levels detected by SPR and nucleic acid molecules rapid flow-through hybridization gene chip (HybriMax) technology were compared.Results Twenty-two kinds of subtypes were detected by SPR.106 women were found with single infection of HPV,including 47 women (44.34％) with abnormal cytological results,while 36 women were found with multiple infection of HPV,including 16 women (44.44％) with abnormal cytological results,and the difference was not significant (P＞0.05).Among 106 women with single infection of HPV,93 women were found with high risk HPV infection,including 45 women (48.39％) with abnormal cytological results,while 13 women were found with middle-low risk HPV infection,including 2 women (15.38％) with abnormal cytological results,and the difference was statistically significant (P＜0.005).Comparison of SPR and HybriMax technology showed that the two test results showed no significant difference (P＞0.05).Conclusions SPR for detection of HPV infection is more reliable,it can combine HybriMax as the primary means of clinical detection.

This article started with the different views to death in Confucianism , Taoism, and Buddhism .And then explore the simple bioethics spirit in Chinese traditional culture , such as cherishing life , equal spirit , the spir-it of benevolence .Finally the research significance is summarized .In the bioethics development era background , researching naive ethics thought in the Chinese traditional culture has important significance to tamp multicultural foundation of bioethics , promote the development of bioethics in China , transform the ancient Chinese bioethics wisdom into the common wealth of mankind and perfectly handle ethical confusion in the development of biology and medicine.

Medical students' practice of filial piety during vacations can help to combine the morality education and filial piety culture for medical students,which is of ethical significance.Firstly,the ethical meanings of filial piety are discussed from three aspects,including "filial piety" and "gratitude",ethical choice of filial piety,and the modern value of filial piety culture.Secondly,ethical reflection on medical students' filial piety education is raised and discussed from three aspects including medical students' well-liking growth and development,cultivating medical ethics and expanding new ways of morality education for medical students.

The sanitary habitation and office are very important for human health, so cleaning is an absolutely necessary part in our living. Although cleaning presents a substantial perceived benefits for our living, there are still some risks for our health. Except for the exposures to dust and other particulate matter suspended during cleaning activities, the main health problem caused by cleaning is the cleaning products. The main hazard composition of cleaning products, air pollution, inhalation pathways, health adverse effects and the measures for reducing the exposures to indoor air pollution caused by cleaning were reviewed in this paper.

Chinese traditional health concepts are rooted in traditional culture,absorb the essence of various health concepts from Confucianism,Buddhism,Daoism and other schools,and form the unique and rich connotation health concepts.These simple concepts of health are historical origin and precious wealth of modem health concepts.Through analyzing the health outlook in traditional books and quoting some thoughts about physical health,mental health,human and social harmony,and ethical health,this paper excavated the character and limitation of traditional health concepts,and explored the enlightenment to modern health ideas.

Objective To observe the curative effect of eliminating epileptic decoction combined with acu-puncture treatment of epilepsy in children .Methods In our hospital in 2013 July to 2014 year in March 70 cases of epileptic children with occult , were randomly divided into two groups .Chinese medicine group of 35 cases were trea-ted with eliminating epileptic decoction combined with acupuncture treatment ;According to the epilepsy type , western medicine group of 35 cases were given appropriate antiepileptic drug treatment .The therapeutic effect was evaluated after 2 months.Results The total effective rate was 88.57%in traditional Chinese medicine group , and in western medicine group, the total effective rate was 74.29%(p<0.05).In group of Chinese medicine and Western medi-cine treatment, the normal rates of EEG were 20% and 17.14% (p>0.05).In the Chinese medicine group after treatment, the normal rate of 51.43%, significantly higher than the control group (p<0.05).The Chinese medicine group after treatment WISC -R score rose slightly .The differences between the two groups were not significant ( p>0.05).The western medicine group arithmetic , understanding, similar score slightly lower but the difference was not significant (p>0.05);the back number rating decreased greatly , compared with those before treatment (p<0.05). Conclusion The elimination of epileptic decoction combined with acupuncture is an effective method for treatment of children with epilepsy , and had no significant effect on intelligence .The curative effect is better than western medi-cine treatment .

BACKGROUND: Colorectal cancer is a common malignant tumor in digestive tract. Theory of tumor stem cells (TSCs) offers a new approach to understand the development mechanism of colorectal cancer. OBJECTIVE: To review the progress in the existence, origin and identification of colorectal cancer stem cells (Co-CSCs). RETRIEVAL STRATEGY: Pubmed was undertaken to identify relevant articles published from January 1990 to July 2007 with the of "colorectal cancer,stem cell,tumor stem cells" in English.At the same time, Vip database was undertaken to identify the relevant articles published between January 1990 and July 2007 with the key words of "colorectal cancer, stem cell, tumor stem cells" in Chinese. The data were selected primarily, and then quotations of each article were checked. Inclusive criterion: the articles related to the advances of the existence, origin and identification of Co-CSCs were selected. Exclusive criterion: the articles with repetitive research were excluded. Totally 63 relevant articles were selected and 33 of them met the inclusive criterion. The 30 excluded articles were of old or repetitive content. LITERATURE EVALUATION: Of the 33 inclusive articles, 28 were on the existence and origin of Co-CSCs, 4 were about the isolation and identification of Co-CSCs, and 1 was about the problems and prospect. DATA SYNTHESIS: TSCs is a rare population of cells in tumor tissues with infinitely proliferative and self-renewal potential that drive the formation and growth of tumors. It may become a new target to eradicate malignant tumor. TSCs have been discovered in leukemia and some solid tumors. Intestine stem cells are poorly differentiated. Its phenotype is difficult to identify. There are many reports on the quantity, location, origin, function and relation with intestinal disease. Co-CSCs have been isolated and identified as EpCAMhigh/CD44+ cells recently, which accelerate to understand the mechanism of colorectal cancer. CONCLUSION: The discovery of Co-CSCs is one of the advances in researching of malignant tumor. TSCs may become a new target to eradicate malignant tumor, and brings hope to cure it.

RNA interference mediated by short interfering RNA is widely used to study functional genes and also being developed for therapeutic applications. However, recent study demonstrated that siRNA might activate innate immune system and induce huge production of inflammatory cytokines in mammals, and also randomly inhibit expression of undesired genes. Designing highly effective siRNAs or modifying the siRNA to retain or enhance the silence efficiency and meanwhile abolish the off-target effects associated with immunostimulation then become the key techniques in application of siRNAs as safe and effective therapeutic agents.