The sanitary habitation and office are very important for human health, so cleaning is an absolutely necessary part in our living. Although cleaning presents a substantial perceived benefits for our living, there are still some risks for our health. Except for the exposures to dust and other particulate matter suspended during cleaning activities, the main health problem caused by cleaning is the cleaning products. The main hazard composition of cleaning products, air pollution, inhalation pathways, health adverse effects and the measures for reducing the exposures to indoor air pollution caused by cleaning were reviewed in this paper.

AIM: To investigate the effects of aging on sperm maturation and fertility in mice. METHODS: Sperm of caput epididymides and cauda epididymides were obtained from Kunming mice aged 6 months (n=15, as control) and 18 months ( n=15). Sperm parameters including sperm density, viability, motility and nor mal morphological rate were recorded. Sperm of cauda epididymides was observed b y transmission electron microscope. The fertility potential and embryo developme ntal competence were performed by in vitro fertilization and embryo culture. RESULTS: Sperm motility, density and normal morphological rate i n aged mice were lower than those in control (P

Objective To describe the public discrimination against patients with mental disorders,and to explore its influencing factors.Methods This study was a descriptive study.A total of 1 378 public received the survey.The survey instruments include general information questionnaire and assessment scale of discrimination against patients with mental disorders.Results The scores of the scale of discrimination against patients with mental disorders was (2.07± 0.61).The score of risk dimension was higher(2.17±0.87),and the ability dimension was lower(1.65±0.64).Multiple regression analysis showed that factors influencing the total score of the scale were type of investigation objects,age,gender,educational background,contact degree and life connection degree to the patients with mental disorders.Conclusion Because psychiatric patients had perceived stigma,and social demographic factors,clinical factors,social and psychological factors could influence their perceived stigma,so the nurse should pay more attention to discrimination against patients,and reduce or eliminate the discrimination toward the patients,then promote recovery of the patients.

Objective To observe the curative effect of eliminating epileptic decoction combined with acu-puncture treatment of epilepsy in children .Methods In our hospital in 2013 July to 2014 year in March 70 cases of epileptic children with occult , were randomly divided into two groups .Chinese medicine group of 35 cases were trea-ted with eliminating epileptic decoction combined with acupuncture treatment ;According to the epilepsy type , western medicine group of 35 cases were given appropriate antiepileptic drug treatment .The therapeutic effect was evaluated after 2 months.Results The total effective rate was 88.57%in traditional Chinese medicine group , and in western medicine group, the total effective rate was 74.29%(p<0.05).In group of Chinese medicine and Western medi-cine treatment, the normal rates of EEG were 20% and 17.14% (p>0.05).In the Chinese medicine group after treatment, the normal rate of 51.43%, significantly higher than the control group (p<0.05).The Chinese medicine group after treatment WISC -R score rose slightly .The differences between the two groups were not significant ( p>0.05).The western medicine group arithmetic , understanding, similar score slightly lower but the difference was not significant (p>0.05);the back number rating decreased greatly , compared with those before treatment (p<0.05). Conclusion The elimination of epileptic decoction combined with acupuncture is an effective method for treatment of children with epilepsy , and had no significant effect on intelligence .The curative effect is better than western medi-cine treatment .

Objective To determine the frequency of GJB2 mutations in the China hearing loss population, and to screen the GJB2 gene in both hearing loss and normal populations. Methods 141 patients with hearing loss and 150 normal persons (control) underwent mutation screening of single coding exon of GJB2 with bidirectional sequencing to identify sequences alterations. Results Three polymorphisms were found: 79G→A, 109G→A, and 341A→G; and four pathologic mutations were identified: 235delC, 455A→G, 176-191del16 and 504insGCAA. Conclusion The 235delC mutation was found to be the significant cause of hearing loss in Chinese population.

BACKGROUND:Artificial lumbar disc replacement is a new choice for the treatment of degenerative disc disease, and preserves lumbar vertebra’s biomechanical characteristics during pain elimination. The design of the prosthesis structure and material needs further study and validation. OBJECTIVE:To review the structure and material types of presently designed artificial lumbar discs, then to discuss the trends in the optimization design of prosthesis. METHODS:The PubMed database, China National Knowledge Infrastructure database and Chinese BioMedical Literature Database were searched for related articles concerning artificial lumbar disc and type and biomechanics of nucleus pulposus prosthesis material published from January 2005 to February 2013 by the first author. Key words were“artificial lumbar disc, principle of prosthesis design, structure, material, clinical trials”in Chinese and“artificial lumbar disc, total disc replacement, structure, material, clinical trial”in English. Repetitive and old studies were excluded. 135 articles were found, but 36 articles were included for review. RESULTS AND CONCLUSION:At present, the materials for intervertebral discs include cobalt-chromium al oy, ceramics, stainless steel, titanium al oy and ultrahigh molecular weight polyethylene. Artificial lumbar disc is commonly made by different materials. Bryan prosthesis is most commonly used in the clinic. Three-dimensional finite element analysis, in vitro trial and clinical studies verified its good biomechanical property. The successful rate of replacement was high. Nucleus prosthesis contains prefabricated type and situ polymerization type, and obtains smal injury, so it is a hot focus in present study, but it cannot achieve biomechanical function of human nucleus pulposus. To dig novel material is a future direction for designing individual prosthesis. The prosthetic structure and biomaterial design experience constant improvement and development. This study combines latest study trend and prospects the development of biomimetic design, material improvement, the optimization design of prosthesis and assisted devices.

Objective:To observe the effect of tuina manipulations on blood pressure and its variability in hypertension patients. Methods:Forty hypertension patients were randomized into an observation group and a medication group, 20 cases in each group. The observation group was intervened by tuina manipulations of kidney-tonifying blood-circulating and collaterals- unblocking in addition to regular medication, while the medication group was by the same medication. The 24-hour blood pressure monitoring was performed before intervention and after 3-month intervention. The blood pressure and its variability were observed and compared. Results:There were no significant differences in comparing the blood pressure and blood pressure variability between the two groups before intervention (P>0.05); after 3-month intervention, the blood pressure and its variability were significantly improved in both groups (P<0.05); the improvements in the observation group were more significant than those in the control group (P<0.05). Conclusion:Tuina manipulations of kidney-tonifying blood-circulating and collaterals-unblocking plus medication can produce a better effect than regular medication in promoting blood pressure and its variability, and this method is worth applying in clinic as it’s easy-to-operate and has no adverse effect.

Objective To investigate the level and influencing factors of caregivers′burden among primary caregivers of elderly stroke patients. Methods Totally 298 caregivers of elderly stroke patients were investigated with Zarit Burden Interview (ZBI), Hospital Anxiety and Depression Scale (HADS) and Simplified Coping Style Questionnaire(SCSQ). The patients were investigated with Modified Barthel Index (MBI). Results The caregivers′burden was in the mild level with a total score of 35.21±11.45. The influencing factors were the number of days of care, daily caring hours, hospital anxiety and depression score, the coping style and the ability of patients to perform ADL. Conclusions The primary caregivers of stroke patients have mild burden. The nurses should be synchronized to assess the health of patients and primary caregivers at the same time, understand the caregivers′burden, and provide specific care advice, in order to reduce the burden of their carer, so as to improve the quality of patients′life.

Objective To investigate the epidemiological characteristics of three common susceptive gene retared hearing loss in the patients with the congenital deafness in Liuzhou.Methods 161 patients with congenital hearing loss were diagnosed with audiologic evolutions,including newborns and outpatients.The blood samples of all patients were taken for the extraction of DNA which was amplified by PCR.The common mutationsl hot spots of the mitochondrial DNA 12SrRNA,GJB2 and SLC26A4 were examined by restricted enzyme and directed sequencing.Results 1 case(0.62%)was found to carry mitoehondrial DNA 12SrRNA A1555G and 4 patients(2.48%)carried heterozygotes or homozygotes pathologic mutations of GJB2.10 patients(6.21%)were heterozygous carriers with pathologic mutations,IVS7-2 A>G,in the SLC26A4 gene.The detection rate of GJB2,mitochondrial DNA A1555G and SLC26A4 mutations in 161 patients were 9.31%.Conclusion The patients with congenital hearing loss distributed different minority groups in liuzhou zone.The mutational frequencies of the three common gene related hearing loss in the patients of Liuzhou were noticeably lower than the data reported in other regions in China.The gene screening for deafness was very important for early diagnosis and treatment.

Objective: To compare the clinical efficacy and safety between etanercept and infliximab in the treatment of adolescent ankylosing spondylitis (AS), and to provide reference for guiding clinical medication. Methods: Sixty male adolescents with AS from our Hospital were used as the subjects and randomly divided into etanercept group and infliximab group (n=30). All patients in the 24 weeks before treatment and during the treatment were not taken the disease-modifying anti-rheumatic drugs (DMARDS). The pillow-wall distance, finger-ground distance, Schober test, AS activity index (BASDIA), AS functional Index (BASFI), C-reactive protein (CRP) levels, erythrocyte sedimentation rate (ESR), MRI performance and other indicators of the patients in two groups were measured and evaluated 12 and 24 weeks after treatment, and statistical analysis was performed to assess the clinical efficacies of the two drugs and the adverse effects were recorded. Results: Etanercept and infliximab had good effects on the AS 24 weeks after treatment, and the pain of low back and lumbosacral of the patients in two groups were significantly reduced or disappeared, and the total clinical efficacies in two groups were 96% and 93%, respectively (P>0. 05). There were significant improvements in the indexes of morning stiffness, finger-ground distance, pillow-wall distance, Schober test, BASDAI scores, BASFI scores, ESR and CRP levels of the patients in two groups 12 and 24 weeks after treatment compared with before treatment (P<0. 05). During treatment, 1 case of erythema was found in etanercept group; 2 cases of moderate infection, 2 cases of digestive system reaction, and 1 case of pruritus were found in infliximab group; the differences in the incidence rates of adverse effects between two groups were statistically significant (P<0. 05). Conclusion: Etanercept and infliximab have good therapeutic effects in the adolescent patients with AS. They can improve the joint symptoms of hip joint involved in AS; the short-term efficacies of the patients in two groups have no significant difference, but the incidence rate of adverse effects of etanercept is slightly less than that of infliximab.