ObjectiveTo discuss the influence of nursing intervention on psychological state of transfusion patients in outpatient and emergency department. MethodsSCL-90 questionnaires were used to investigate 600 transfusion patients in outpatient and emergency department to understand their psychological state. Psychological nursing was given to them before, during and after transfusion, SCL- 90 questionnaire was used again for investigation at the last day of transfusion,χ2 test was used to evaluate the results. ResultsAfter psychological care, the somatization, interpersonal relationships, anxiety, depres-sion, hostility and terrorism and other items significantly alleviated compared with those before interven-fion. ConclusionsTo grasp the patients' psychological state and give psychological care to them can ef-fectively reduce the adverse psychological reaction of patients, improve the quality of care and safety and promote the treatment and rehabilitation of diseases.

Objective:To investigate the expression of CD14 +/CD16 + by monocytes in neonate septicemia and its significance.Methods:The expression of CD14 +/CD16 + by monocytes in 124 neonates was analyzed by Flow cytometry,the levels of IL 6、IL 10 and TNF ? in blood was measured by radioimmunoassay and bacteria culture and drug sensitive were tested by Bactec 9120 bacteria culture system.Results:The expression levles of CD14 +/CD16 + by monocytes and the levels of IL 6 and TNF ? in the septicemia group were significantly higher than those in the normal group and non septicemia group (P

Objective:To investigate the expression of membrane cofacto protein(MCP)?decay accelerating factor(DAF) and homologous restricition factor 20(HRF 20) on the surface of CD16 + monocyte/macrophage(Mo/M?) in patients with glomerulonephritis(GN).Methods:Tested the expression levels of MCP?DAF and HRF 20 on the surface of CD16 +Mo/M? by Flow Cytometry and the levels of serum C3d and C3d immunologic complex(C3d IC) by ELISA in 136 patients with GN,divided into mini change(MC)?glomerulosclerosis(GS)?membranous nephropathy(MN) and proliferative glomerulonephritis(PGN).Results:The expressions of MCP?DAF and HRF 20 on the surface of CD16 +Mo/M? and the serum levels of C3d in the patients with GS?MN or PGN were significantly higher than those in controls(P

Objective To investigate the differences in the gene expression profiles between SW480 and SW620 cell lines.Methods A dataset of GDS756 containing the gene expression profiles of SW480 and SW620 was downloaded from the GEO database in NCBI.The differential expression genes between SW480 and SW620 were analyzed with gene set enrichment analysis (GSEA) and leading edge subset analysis.The genes in leading edge subset were re-annotated by FunRich software.The core genes of leading edge subset closely relating to SW480 or SW620 were analyzed with the STRING on-line analytical system.The functional core genes closely relating to SW480 or SW620 were obtained by the combined analysis of the core genes and high frequency genes from leading edge subset.Results GSEA identified 12 significantly enriched gene sets,491 leading edge genes and 7 highly overlapping genes from SW480 and 80 significantly enriched gene sets,870 leading edge genes and 6 highly overlapping genes from SW620.The STRING system identified 5 core genes from SW480 and 8 from SW620.The combined analysis of GSEA and bionetwork obtained 2 functional core genes,TOP2A and CDK1,from SW620.Conclusion The SW480 and SW620 cells with identical genetic background have different functional gene expression profiles,and the functional core genes TOP2A and CDK1 in SW620 cells may be related to the signal pathways of colon cancer metastasis.

Objective:To explore the latent profile and characteristics of social isolation in patients with hematologic malignancy, and to analyze its related influencing factors, and to provide reference for improving social phobia disorder in different patients and implementing targeted intervention.Methods:This study was a cross-sectional survey. A convenient sampling method was used to select hematologic malignancy patients who were treated in Qilu Hospital of Shandong University from January 2022 to January 2023. General information questionnaire, the General Alienation Scale (GAS), and the Social Support Rating Scale(SSRS) were used for investigation. Latent profile was analyzed using the categories of social isolation in patients with hematologic malignancy, and univariate and multinomial logistic regression analysis were used to analyze relevant influencing factors.Results:A total of 195 survey subjects were included, of which 108 males and 87 females, aged (49.78 ± 13.52) years. The scores of GAS and SSRS were (43.21 ± 6.09) and (42.52 ± 6.77) respectively. The social isolation in patients with hematologic malignancy could be divided into 3 latent profiles, namely low-risk isolation 15.4% (30/195), medium-risk isolation 68.2%(133/195), and high-risk isolation16.4% (32/195). Multinomial Logistic regression analysis showed that age ( OR=0.941, 95% CI 0.894-0.990), percapita monthly income of families ( OR=0.050, 95% CI 0.004-0.657), primary caregivers (parents) ( OR=0.025, 95% CI 0.003-0.227), place of residence (town)( OR=0.170, 95% CI 0.039-0.749), disease type (leukemia) ( OR=15.610, 95% CI 2.973-81.979), disease type(lymphoma) ( OR=10.986, 95% CI 2.032-59.413) were the influencing factors of medium-risk isolation (all P<0.05). Age ( OR=0.933, 95% CI 0.880-0.988), percapita monthly income of families ( OR=0.029, 95% CI 0.002-0.525), primary caregivers (parents) ( OR=0.076, 95% CI 0.006-0.900), disease type (leukemia)( OR=19.257, 95% CI 2.580-143.723), disease type (lymphoma)( OR=9.952, 95% CI 1.290-76.763), social support ( OR=0.877, 95% CI 0.786-0.980) were the influencing factors of high-risk isolation (all P<0.05). Conclusions:The social isolation among patients with hematologic malignancy had apparent classification characteristics. It could be divided into three potential profiles. It is suggested that medical staff should take targeted social and psychological support based on different types of patients, improve their psychological and social outcomes, and utilize existing resources to implement intervention measures to help them adapt and return to society.

Objective To explore the effect of individual tracer methodology in clinical nursing quality control.Methods By founding the supervision inspection group and evaluation standard , patients were followed up with questions (including access to medical records , ask the nurse, field view, the conversation, satisfaction survey, etc.), and continued to track the problems , so as to improve nursing measures and resolve the issues to enhance patient satisfaction and quality of care .Results After the implementation of case follow -up, the score of quality nursing for the department of respiratory and neurology was improved from (90.31 ±2.21) and (91.80 ±1.66) to (97.14 ±1.99) and (98.23 ±1.48).There were significantly difference (t=-7.965,-7.341, respectively;P <0.05 ).The comprehensive quality nursing score significantly improved from (91.63 ±3.78) to (97.64 ±1.63) after six months (t=-5.138,P<0.05).Patient satisfaction improved from (87.88 ±3.08)% to (95.53 ±1.70)%.There was significant improvement of the satisfaction (t =-17.411,P<0.05).Conclusions The individual tracking control method for nursing quality control's effect is obvious.It can effectively promote the implementation of high quality nursing service , and it is a relatively comprehensive and systematic nursing quality control method , so it is worthy of popularizing in clinical .

Objective To observe the feasibility of low tube voltage scanning technology in CT-guided cryoablation therapy of liver cancer.Methods Twenty-one patients who underwent routine CT(tube voltage 120 kV)-guided cryoablation of liver cancer(group A)were retrospectively enrolled,while low tube voltage(100 kV)CT-guided cryoablation of liver cancer was prospectively performed in 23 patients(group B).CT scanning were performed before and after ablation with automatic tube current mode(50-450 mA),and adaptive statistical iterative reconstruction(ASIR)algorithm was used for image reconstruction(reconstruction percentages of 40%)of both groups.The subjective scores and objective evaluations of CT image quality before and after treatment were compared between groups,so as the treatment effect and patient's radiation dose(volume CT dose index[CTDIvol]).Results No significant difference of subjective scores,objective evaluations of CT image quality nor treatment effect was detected between groups(all P＞0.05).Before and after treatment,CTDIvol of patients in group A was significantly higher than that in group B(btoh P＜0.001).Conclusion Low tube voltage scanning technology could be used for CT-guided cryoablation of liver cancer,which might ensure image quality without affecting evaluation on cryoablation effect and reduce radiation dose combined with ASIR algorithm.

Objective:To explore the current status and influencing factors of alexithymia among elderly stroke patients.Methods:From August 2017 to April 2018, we investigated 200 elderly stroke inpatients in Neurology Department at a ClassⅢ Grade A hospital in Henan with the self-designed General Information Questionnaire, Toronto Alexithymia Scale 20 (TAS-20) , Social Support Rate Score (SSRS) and Hamilton Depression Scale (HAMD) .Results:Among those stroke patients, the score of alexithymia was (67.42±9.13) and the incidence rate was 51%, which was mainly manifested in extroverted thinking and recognition emotional disorders. Multiple stepwise regression analysis showed that the main influencing factors of alexithymia in elderly stroke patients were the age, hemiplegia, aphasia, social support and depression, which could explain 50.6% of the variation in alexithymia.Conclusions:Alexithymia of elderly stroke patients needs to be improved. While providing nursing care for elderly stroke patients, clinical nursing workers and family members of patients should pay attention to patients' emotional expression, thinking style and depression, especially for patients with advanced age, hemiplegia, aphasia, loss of self-care ability and so on. On the other hand, social support can also be used to improve patients' alexithymia so as to reduce complications, improve clinical outcomes and their quality of life.