Objective The current status of achievement motive levels and autonomous learning ability of nursing students was studied and compared in order to put forward suggestions to improve the achievement motive levels accordingly.Methods 119 nursing students were investigated about their achievement motive levels and autonomous learning ability by questionnaires.Then these students were divided into three groups according to their achievement motive scores and the autonomous learning ability was compared.Results The scores of Success-oriented motive of nursing students were significantly higher than that of failure-threatened motive.The total score of autonomous learning ability was(88.653+9.373)and the autonomous learning ability among these three groups was statistically different(P＜0.01).Conclusion The achievement motive greatly influenced the autonomous learning ability of nursing students.Measures should be adopted to improve the achievement motives so as to enhance their autonomous learning ability.

Objective:To investigate the expression of CD14 +/CD16 + by monocytes in neonate septicemia and its significance.Methods:The expression of CD14 +/CD16 + by monocytes in 124 neonates was analyzed by Flow cytometry,the levels of IL 6、IL 10 and TNF ? in blood was measured by radioimmunoassay and bacteria culture and drug sensitive were tested by Bactec 9120 bacteria culture system.Results:The expression levles of CD14 +/CD16 + by monocytes and the levels of IL 6 and TNF ? in the septicemia group were significantly higher than those in the normal group and non septicemia group (P

Objective:To investigate the expression of membrane cofacto protein(MCP)?decay accelerating factor(DAF) and homologous restricition factor 20(HRF 20) on the surface of CD16 + monocyte/macrophage(Mo/M?) in patients with glomerulonephritis(GN).Methods:Tested the expression levels of MCP?DAF and HRF 20 on the surface of CD16 +Mo/M? by Flow Cytometry and the levels of serum C3d and C3d immunologic complex(C3d IC) by ELISA in 136 patients with GN,divided into mini change(MC)?glomerulosclerosis(GS)?membranous nephropathy(MN) and proliferative glomerulonephritis(PGN).Results:The expressions of MCP?DAF and HRF 20 on the surface of CD16 +Mo/M? and the serum levels of C3d in the patients with GS?MN or PGN were significantly higher than those in controls(P

Objective To investigate the effects of exercise on memory impairment in demented mice and possible molecular mechanisms.Methods Thirty Kunming nice were divided into a normal control group,a dementia group and a dementia plus exercise training group (exercise training group),10 mice/group. Dementia models were established by intraperitoneal injection of scopolamine.Exercise training was begun 30 min after the injection and lasted for a 6-week period. A water maze test was performed after the exercise training. Brain-derived neurotrophic factor (BDNF) expression and protein kinase (Akt) phosphorylation in the hippocampus were detected by Western blot analysis.Nitric oxide (NO) content and superoxide dismutase (SOD) activity in the hippocampus were also measured.ResultsDementia models were successfully induced as indicated by prolonged escape latency in the water maze test.After exercise training the escape latency had shortened significantly and hippocanpal BDNF expression and Akt phosphorylation had increased.NO content increased and SOD activity decreased after the injections,but these effects were partially reversed after exercise training.Conclusions Exercise can improve spatial memory significantly after scopolanine-induced dementia in mice.The increased hippocampal BDNF expression and Akt phosphorylation,increased SOD activity and decreased NO content may partially explain the behavior improvement induced by exercise.

Objective :To analyze and explore influence of hyperuricemia (HUA ) on intracranial atherosclerosis . Methods : A total of 250 patients with non—cardiogenic cerebral infarction treated in our hospital from May 2014 to Jul 2016 were enrolled .Digital subtraction angiogrophy was performed in all patients .According to level of serum uric acid (SUA) ,patients were divided into HUA group (n＝84) and normal UA group (n＝166).Age ,percentages of hypertension ,coronary heart disease ,diabetes mellitus (DM) , hyperlipodemia ,smoking history and recurrence rate of cerebral infarction were compared between two groups ,and influencing factors of atherosclerotic intracranial artery stenosis were analyzed using single—and multi—factor regression analysis .Results : Compared with normal UA group ,there was significant rise in recurrence rate of ischemic stroke (25.30% vs.45.24%) in HUA group , P＝0.001. Single factor analysis indicated that hypertension ,DM and HUA were influencing factors for intracranial ar—tery stenosis , P＝0.001 all ;multi—factor regression analysis indicated that hypertension ,DM and HUA were inde—pendent risk factors for atherosclerotic intracranial artery stenosis (OR＝0. 960～4.957 , P＝0.001 all).Conclusion :Hyperuricemia is an independent risk factor for intracranial atherosclerotic stenosis .Monitor on HUA patients should be enhanced in clinic in order to prevent intracranial atherosclerosis .

Objective To observe the feasibility of low tube voltage scanning technology in CT-guided cryoablation therapy of liver cancer.Methods Twenty-one patients who underwent routine CT(tube voltage 120 kV)-guided cryoablation of liver cancer(group A)were retrospectively enrolled,while low tube voltage(100 kV)CT-guided cryoablation of liver cancer was prospectively performed in 23 patients(group B).CT scanning were performed before and after ablation with automatic tube current mode(50-450 mA),and adaptive statistical iterative reconstruction(ASIR)algorithm was used for image reconstruction(reconstruction percentages of 40%)of both groups.The subjective scores and objective evaluations of CT image quality before and after treatment were compared between groups,so as the treatment effect and patient's radiation dose(volume CT dose index[CTDIvol]).Results No significant difference of subjective scores,objective evaluations of CT image quality nor treatment effect was detected between groups(all P＞0.05).Before and after treatment,CTDIvol of patients in group A was significantly higher than that in group B(btoh P＜0.001).Conclusion Low tube voltage scanning technology could be used for CT-guided cryoablation of liver cancer,which might ensure image quality without affecting evaluation on cryoablation effect and reduce radiation dose combined with ASIR algorithm.

Objective:To investigate the role of plasma neurofilament light chain (NfL) in diagnosing and differentiating Parkinson's disease (PD) and multiple system atrophy-Parkinsonian subtype (MSA-P).Methods:Forty PD patients and 23 MSA-P patients admitted to Department of Neurology, Henan Provincial People's Hospital from June 2019 to December 2021 were recruited; 27 healthy subjects accepted physical examination during the same period were selected as controls. Ultrasensitive Simoa technology was used to measure the plasma NfL. Differences in clinical data and plasma NfL were compared among all subjects. Correlations of plasma NfL with clinical characteristics, such as disease course, Hoehn-Year (H-Y) staging, Unified Parkinson's Disease Rating Scale (UPDRS), Montreal Cognitive Assessment (MoCA), Scale for Outcomes in Parkinson's Disease for Autonomic Symptoms (SCOPA-AUT) and levodopa equivalent daily dosage (LEDD), were analyzed with Pearson correlations. Receiver operating characteristic (ROC) curve was used to analyze the value of plasma NfL in diagnosing and differentiating PD and MSA-P.Results:Compared with MSA-P group, PD group had significantly longer disease course and statistically lower scores of UPDRS-II and SCOPA-AUT ( P<0.05). The plasma NfL in MSA-P group, PD group and healthy control group was decreased successively ([37.69±10.47] pg/mL, [17.85±4.23] pg/mL, [12.86±3.14] pg/mL, respectively), with statistical differences ( P<0.05). In MSA-P patients, Pearson correlations showed positive correlation between plasma NfL and age ( r=0.442, P=0.035); and Partial correlations showed positive correlations between plasma NfL and scores of UPDRS-I and UPDRS-III ( P<0.05), and plasma NfL showed no significant correlation with H-Y staging, UPDRS-III, MoCA, LEDD or SCOPA-AUT scores ( P>0.05). In PD patients, Pearson correlations showed that plasma NfL was positively correlated with age ( r=0.342, P=0.031); partial correlations showed that plasma NfL was positively correlated with H-Y staging and UPDRS-III, and negatively correlated with MoCA scores ( P<0.05); plasma NfL showed no significant correlation with disease course, scores of UPDRS-I and UPDRS-II, LEDD, and SCOPA-AUT scores ( P>0.05). ROC curve showed that the area under the curve (AUC) of plasma NfL in diagnosing PD was 0.814 (95% CI: 0.712-0.920, P<0.001); AUC of plasma NfL in differentiating and diagnosing PD and MSA-P was 0.980 (95% CI: 0.954-1.000, P<0.001); AUC of plasma NfL in diagnosing MSA-P was 0.998 (95% CI: 0.993-1.000, P<0.001). Conclusions:Plasma NfL is correlated with severity of motor symptoms in MSA-P patients; plasma NfL is correlated with cognitive function and disease course in PD patients. Besides, plasma NfL has high sensitivity and specificity in differentiating PD and MSA-P, therefore, plasma NfL could serve as a biomarker to diagnosis and differentiate PD.