Objective To investigate the effects of exercise on memory impairment in demented mice and possible molecular mechanisms.Methods Thirty Kunming nice were divided into a normal control group,a dementia group and a dementia plus exercise training group (exercise training group),10 mice/group. Dementia models were established by intraperitoneal injection of scopolamine.Exercise training was begun 30 min after the injection and lasted for a 6-week period. A water maze test was performed after the exercise training. Brain-derived neurotrophic factor (BDNF) expression and protein kinase (Akt) phosphorylation in the hippocampus were detected by Western blot analysis.Nitric oxide (NO) content and superoxide dismutase (SOD) activity in the hippocampus were also measured.ResultsDementia models were successfully induced as indicated by prolonged escape latency in the water maze test.After exercise training the escape latency had shortened significantly and hippocanpal BDNF expression and Akt phosphorylation had increased.NO content increased and SOD activity decreased after the injections,but these effects were partially reversed after exercise training.Conclusions Exercise can improve spatial memory significantly after scopolanine-induced dementia in mice.The increased hippocampal BDNF expression and Akt phosphorylation,increased SOD activity and decreased NO content may partially explain the behavior improvement induced by exercise.

Objective To investigate platelet α and β secretase activities and the amounts of platelet soluble fragment of APP (sAPPα) produced by α-secretase in patients with mild cognitive impairment (MCI) and Alzheimer's disease (AD).Methods The neurological functions of 48 nondemented patients,42 MCI and 40 AD patients were evaluated by neuropsychological examinations.The platelet α and β secretase activities and sAPPα production in each group were measured by fluorescence and Western blotting analysis respectively.Results The α secretase activities in non-demented,MCI and AD group were 100.0％ ± 10.6％,78.2％ ± 9.4％ and 61.8％ ± 7.2％ respectively.As compared with nondemented group,the α secretase activities in MCI and AD group were decreased (F =22.935,P =0.001).The α secretase activity in AD group was significantly lower than MCI group.The β secretase activities in non-demented,MCI and AD group were 100.0％ ± 11.2％,145.8％ ± 12.7％ and 189.8％ ± 14.2％respectively.The β secretase activities in MCI and AD group were significantly higher than that in nondemented group (F =16.368,P =0.001).The β secretase activity in AD group was significantly decreased as compared with MCI group.The sAPPα amounts in MCI group and AD group were all decreased as compared with that in control group; the sAPPo amount in AD patients was significantly decreased as compared with that in MCI group.Conclusions The platelet α secretase activity and its production sAPPα in MCI and AD patients are decreased,while β secretase activity is increased,as compared with that in control group; the altered α and β secretase activities may participate in the pathogenesis of MCI and AD patients and may have diagnostic potential for them.

Objective? To explore the effects of graphic communication and education on cleanliness of intestinal preparation and detection rate of colonic polyposis in patients. Methods? Totally 96 patients receiving colonoscopy in He'nan Provincial People's Hospital from May 2016 to May 2018 were selected by convenient sampling, and divided into the control group (n=48) and the observation group (n=48). Patients in the control group took Polyethylene Glycol Electrolytes Powder orally for conventional intestinal preparation, while patients in the observation group received graphic communication and health on the basis of conventional intestinal preparation. Intestinal cleanliness, duration of colonoscopy, acceptance rate of repeated intestinal preparation and detection rate of colonic polyposis were compared between the two groups, and the incidence of adverse effects was analyzed. Results? The good rate of intestinal cleanliness in the observation group was 91.67%, while that in the control group was 75.00%, and the difference was statistical significant (P＜ 0.05); the duration of colonoscopy in the observation group was (7.24±2.16) min, shorter than that in the control group, which was (9.01±3.54) min, and the difference was statistical significant (P＜ 0.05); the acceptance rate of repeated intestinal preparation and the detection rate of colonic polyposis in the observation group were 4.17% and 29.17%, whereas those of the control group were 18.75% and 12.50%, respectively, and the difference was statistical significant (P＜ 0.05); the rate of adverse effects in the observation group was 14.58%, lower than that of the control group, which was 52.08%, and its incidence rate of abdominal distention and stomachache was lower than that of the control group, and the differences were all statistical significant (P＜0.05). Conclusions? The graphic communication and education can improve the intestinal cleanliness and the detection rate of colonic polyposis by colonoscopy in patients.

This paper reviewed literature at home and abroad, clarified the definition of drug literacy, elucidated the association between drug literacy and secondary preventive drug in stroke patients, analyzed the feasibility and limitations of existing drug literacy assessment tools in stroke patients, summarized the research status of drug literacy in stroke patients, so as to provide reference for the evaluation and intervention of drug literacy in stroke patients in China.

OBJECTIVE: To use the maximum loudest phonation time (MLPT) on evaluation of unilateral vocal fold paralysis. METHOD: The MLPT, maximum comfortable phonation time (MCPT) and maximum phonation time (MPT) were tested and collected in 17 patients with unilateral vocal fold paralysis. The data of ratio value of MLPT to MCPT also was collected. The stroboscopy, perceptual measures and self-questionnaire also were used in evaluation of vocal fold and voice in all patients. Correlation coefficients were used as measures of agreement. RESULT: MLPT was (5.0 +/- 4.0)s, MCPT was (5.4 +/- 4.1)s, and MPT was (6.1 +/- 4.5)s in patients. The MPT was composed of MLPT in 4 patients and MCPT in 13 patients. The MLPT/MCPTa was 1.08 +/- 0.47. MLPT was positively correlated with MCPT and MPT, respectively (r = 0.679, P < 0.01; r = 0.878, P < 0.01), and MCPT also was positively correlated with MPT (r = 0.993, P < 0.01). MLPT, MCPT and MPT was negatively correlated with G value which was from GRBAS scale, respectively (r = -0.620, P < 0.05; r = -0.564, P < 0.05; r = -0.665, P < 0.05). The MLPT/MCPTa was positively correlated with the value of question 4 from self-questionnaire (r = 0.534, P < 0.05). MLPT, MCPT or MPT had no correlation with self-questionnaire, GRBAS perceptual evaluation or stroboscopy measures significantly. CONCLUSION MLPT, MCPT or MPT can be used for evaluation of aerodynamic measures in unilateral vocal fold paralysis. The MLPT may be easiest to operate in clinic. The MLPT/MCPTa ratio can assess dysphagia in unilateral vocal fold paralysis patients.
Adolescent ; Adult ; Female ; Humans ; Male ; Middle Aged ; Phonation ; Vocal Cord Paralysis ; pathology ; physiopathology ; Vocal Cords ; pathology ; Voice Quality ; Young Adult

Objective:To investigate the relationship between plasma phosphorylated α-synuclein (ps129-α-syn) and cognitive function in Parkinson disease (PD).Methods:This study recruited 90 PD patients who were hospitalized in the Department of Neurology, Henan province people's hospital from March 2019 to June 2020.Forty healthy middle-aged and elderly people with normal cognitive function who came to the hospital for physical examination were selected during the same period.Clinical characteristics and blood samples were collected.Patients with PD were classified into those with normally cognitive (PD-NC), mild cognitive impairment (PD-MCI), and dementia (PDD). The enzyme-linked immunosorbent assay was used to measure the plasma ps129-α-syn.Correlations between plasma ps129-α-syn and clinical characteristics such as disease duration, Hoehn-Yahr stage (H-Y), unified Parkinson's disease rating scale (UPDRS), Montreal cognitive assessment (MoCA), 14-item Hamilton anxiety rating scale (HAMA-14), the 24-item Hamilton depression rating scale (HAMD-24), levodopa equivalent daily dosage (LEDD), the scale of outcomes in Parkinson's disease for autonomic symptoms, SCOPA-AUT) were analyzed using Pearson or Spearman correlation analysis.Multiple linear regression analysis was used to evaluate the factors affecting the cognitive function of PD.Results:Plasma ps129-α-syn in PD patients was higher than that in healthy controls((19.44±8.93)μg/L, (10.78±5.87)μg/L, ( t=5.615, P<0.01). Plasma ps129-α-syn was higher in PD-MCI group((19.64±7.77)μg/L)and PDD group((23.79±9.47)μg/L) compared with that in PD-NC group((13.37±5.40)μg/L)( P<0.05). Plasma ps129-α-syn was positively correlated with H-Y ( r=0.404, P<0.01), UPDRS-Ⅲ( r=0.275, P=0.009), UPDRS-total ( r=0.211, P=0.046) and SCOPA-AUT( r=0.335, P=0.001). Plasma ps129-α-syn was negatively associated with MoCA ( r=-0.459, P<0.01). Multiple linear regression analysis suggested disease duration ( t=-4.618, P<0.01), ps129-α-syn( t=-3.792, P<0.01) and UPDRS-total ( t=-2.826, P=0.006) were independently associated with cognitive function.Plasma ps129-α-syn could discriminate between PD-NC and PD cognitive function impairment with an AUC of 0.7797 (95% CI: 0.686 3-0.873 2, P<0.01). Conclusions:Plasma ps129-α-syn is correlated with cognitive function and the severity of motor symptoms in PD patients, and have high sensitivity and specificity in diagnosing PD cognitive dysfunction.Therefore, plasma ps129-α-syn can serve as a biomarker to assess cognitive function in PD.

Objective To explore the effect of individual tracer methodology in clinical nursing quality control.Methods By founding the supervision inspection group and evaluation standard , patients were followed up with questions (including access to medical records , ask the nurse, field view, the conversation, satisfaction survey, etc.), and continued to track the problems , so as to improve nursing measures and resolve the issues to enhance patient satisfaction and quality of care .Results After the implementation of case follow -up, the score of quality nursing for the department of respiratory and neurology was improved from (90.31 ±2.21) and (91.80 ±1.66) to (97.14 ±1.99) and (98.23 ±1.48).There were significantly difference (t=-7.965,-7.341, respectively;P <0.05 ).The comprehensive quality nursing score significantly improved from (91.63 ±3.78) to (97.64 ±1.63) after six months (t=-5.138,P<0.05).Patient satisfaction improved from (87.88 ±3.08)% to (95.53 ±1.70)%.There was significant improvement of the satisfaction (t =-17.411,P<0.05).Conclusions The individual tracking control method for nursing quality control's effect is obvious.It can effectively promote the implementation of high quality nursing service , and it is a relatively comprehensive and systematic nursing quality control method , so it is worthy of popularizing in clinical .

Frailty in elderly patients undergoing arthroplasty increases the risk of postoperative complications, prolongs hospital stays, delays the rehabilitation process, and aggravates the economic burden. Frailty is a dynamic condition, and early detection and effective intervention can delay its progression. Therefore, early assessment of frailty status in patients is necessary. Currently, there are numerous frailty assessment tools, but the selection of these tools lacks a solid basis. This paper reviews the impact of frailty on the physical condition of elderly arthroplasty patients, as well as the content, application, advantages, and limitations of existing frailty assessment tools at home and abroad. Furthermore, it summarizes the problems in the process of frailty assessment and puts forward prospects, aiming to provide reference for the identification and assessment of frailty in elderly arthroplasty patients in the future.

Objective:To investigate the role of plasma neurofilament light chain (NfL) in diagnosing and differentiating Parkinson's disease (PD) and multiple system atrophy-Parkinsonian subtype (MSA-P).Methods:Forty PD patients and 23 MSA-P patients admitted to Department of Neurology, Henan Provincial People's Hospital from June 2019 to December 2021 were recruited; 27 healthy subjects accepted physical examination during the same period were selected as controls. Ultrasensitive Simoa technology was used to measure the plasma NfL. Differences in clinical data and plasma NfL were compared among all subjects. Correlations of plasma NfL with clinical characteristics, such as disease course, Hoehn-Year (H-Y) staging, Unified Parkinson's Disease Rating Scale (UPDRS), Montreal Cognitive Assessment (MoCA), Scale for Outcomes in Parkinson's Disease for Autonomic Symptoms (SCOPA-AUT) and levodopa equivalent daily dosage (LEDD), were analyzed with Pearson correlations. Receiver operating characteristic (ROC) curve was used to analyze the value of plasma NfL in diagnosing and differentiating PD and MSA-P.Results:Compared with MSA-P group, PD group had significantly longer disease course and statistically lower scores of UPDRS-II and SCOPA-AUT ( P<0.05). The plasma NfL in MSA-P group, PD group and healthy control group was decreased successively ([37.69±10.47] pg/mL, [17.85±4.23] pg/mL, [12.86±3.14] pg/mL, respectively), with statistical differences ( P<0.05). In MSA-P patients, Pearson correlations showed positive correlation between plasma NfL and age ( r=0.442, P=0.035); and Partial correlations showed positive correlations between plasma NfL and scores of UPDRS-I and UPDRS-III ( P<0.05), and plasma NfL showed no significant correlation with H-Y staging, UPDRS-III, MoCA, LEDD or SCOPA-AUT scores ( P>0.05). In PD patients, Pearson correlations showed that plasma NfL was positively correlated with age ( r=0.342, P=0.031); partial correlations showed that plasma NfL was positively correlated with H-Y staging and UPDRS-III, and negatively correlated with MoCA scores ( P<0.05); plasma NfL showed no significant correlation with disease course, scores of UPDRS-I and UPDRS-II, LEDD, and SCOPA-AUT scores ( P>0.05). ROC curve showed that the area under the curve (AUC) of plasma NfL in diagnosing PD was 0.814 (95% CI: 0.712-0.920, P<0.001); AUC of plasma NfL in differentiating and diagnosing PD and MSA-P was 0.980 (95% CI: 0.954-1.000, P<0.001); AUC of plasma NfL in diagnosing MSA-P was 0.998 (95% CI: 0.993-1.000, P<0.001). Conclusions:Plasma NfL is correlated with severity of motor symptoms in MSA-P patients; plasma NfL is correlated with cognitive function and disease course in PD patients. Besides, plasma NfL has high sensitivity and specificity in differentiating PD and MSA-P, therefore, plasma NfL could serve as a biomarker to diagnosis and differentiate PD.

Objective:To explore the current status and influencing factors of alexithymia among elderly stroke patients.Methods:From August 2017 to April 2018, we investigated 200 elderly stroke inpatients in Neurology Department at a ClassⅢ Grade A hospital in Henan with the self-designed General Information Questionnaire, Toronto Alexithymia Scale 20 (TAS-20) , Social Support Rate Score (SSRS) and Hamilton Depression Scale (HAMD) .Results:Among those stroke patients, the score of alexithymia was (67.42±9.13) and the incidence rate was 51%, which was mainly manifested in extroverted thinking and recognition emotional disorders. Multiple stepwise regression analysis showed that the main influencing factors of alexithymia in elderly stroke patients were the age, hemiplegia, aphasia, social support and depression, which could explain 50.6% of the variation in alexithymia.Conclusions:Alexithymia of elderly stroke patients needs to be improved. While providing nursing care for elderly stroke patients, clinical nursing workers and family members of patients should pay attention to patients' emotional expression, thinking style and depression, especially for patients with advanced age, hemiplegia, aphasia, loss of self-care ability and so on. On the other hand, social support can also be used to improve patients' alexithymia so as to reduce complications, improve clinical outcomes and their quality of life.