[Objective] To compare the disinfection effect of different disinfectants on disinfection effect of laryngeal endoscope,and seek for a more safe and effective disinfectant for laryngeal endoscope.[Methods] 180 laryngeal endoscope were divided into three groups with 60 of them in each group.Acidic electrolyzed oxidizing water(AEOW),2％ glutaraldehyde and orthophthalaldehyde were used for disinfection.Three minutes and five minutes after disinfection,the samples were got and underwent bacterial detection.The disinfection effect after three minutes and five minutes was compared.[Results] The qualified rate of disinfection and cost between AEOW,2％ glutaraldehyde and orthophthalaldehyde showed distinct difference.[Conclusions] Using acidic electrolyzed oxidizing water in the disinfection of laryngeal endoscope has advantages of fast,effective,low-cost,which can be applied in the clinical work.

Objective To test and evaluate measurement uncertainty and relational performance parameters of the verification device for laser protective spectacles.Methods By using realtime beam-splitting surveillance,several performance parameters,including the measurement repeatability of the verification device,optical density of laser protective spectacles in different laser exposure conditions and its damage threshold,were tested.The measurement modules of the main performance parameters were built up as the experiment & technology platform for performance evaluation of laser protective spectacles and for measurement uncertainty appraisal of verification device.Results Laser wavelengths of the verification device were 1064nm and 532nm,response range of wavelength was from 0.4?m to 1.1?m,measuring range of laser energy was from 10-8 J to 0.3J,measuring range of optical density for laser protective spectacles was from 0.1 to 8.0,measuring repeatability was 0.37%,and measuring uncertainty was 5% (k =2).Conclusion The automatic verification device is steady and reliable.The achieved performance indexes accord with the designed requirement for laser protection.

BACKGROUND:Neural stem cel transplantation has been used to treat a series of brain injury diseases, such as cerebral palsy, but its effect on Alzheimer’s disease is rarely reported. OBJECTIVE:To observe the effect of neural stem cel transplantation on the behavior and immune regulating system of Alzheimer’s disease rats. METHODS:Thirty-five Sprague-Dawley rats were enrol ed to make a postcerebral incision and given hippocampal injection of amanita phal oides acid to establish rat models of Alzheimer’s disease. Another 10 rats were only given hippocampal injection of normal saline after preparation of postcerebral skin incision as sham operation group. Then 32 successful rat models were randomly divided into two groups (n=16 per group):rats in experimental group were administrated hippocamal injection of 5×109/L al ogeneic neural stem cel suspension;those in model group were given no injection. Five-day Morris water maze test was conducted at 4 weeks after transplantation. At 1 week after Morris water maze test, levels of interleukin-1 and interleukin-10 in the cerebral homogenate were detected, as wel as pathological changes of brain tissues were observed in the three groups. RESULTS AND CONCLUSION:Compared with the model group, the abilities of cognition and memory were significantly higher in the sham operation group (P<0.01), and the abilities of spatial learning and memory were significantly higher in the experimental group (P<0.05, P<0.01). Levels of interleukin-1 and interleukin-10 in the model group were significantly higher than those in the sham operation group (P<0.01) but significantly lower than those in the experimental group ( P<0.01). Besides, the number of neurons in the model group was obviously less than that in the experimental and sham operation group. These results indicate that neural stem cell transplantation supplements and protects neurons against Alzheimer's disease in rats, thereby significantly improving the learning and memory ability.

ObjectivesTo investigate the clinical value of the melting curve analysis-based PCR assay for the clinical genetic diagnosis and prenatal diagnosis of β-thalassemia.Methods A total of 451 peripheral blood samples,including 372 cases with β-thalassemia phenotypes and 79 cases without β-thalassemia phenotypes,were collected by Liuzhou Municipal Maternity and Child Healthcare Hospital between January 2011 and August 2011.Moreover,another 84 cases,including 16 fetal villi samples (10 - 13 weeks),64 amniotic fluid samples (16 -24 weeks ) and 4 umbilical cord blood samples (above 17 weeks),whose parents were β-thalassemia carriers,were also collected for this assay between June 2011 and September 2011.A double-blind test was done to compare the detection reliability of the melting curve analysis-based assay (24 β-thalassemia mutations can be detected) with PCR-RDB probe assay (17 β-thalassemia mutations can be detected ) and DNA sequencing using these samples.The wildtype,mutant and total concordance rates of the genotyping results were calculated separately among the melting curve analysis based assay,PCR-RDB probe assay and DNA sequencing.Results Among the 451 peripheral blood samples,thirteen mutations and nineteen genotypes were obtained by using melting curve analysis-based assay.447 samples had the same detection results and 4 samples had different detection results by comparing melting curve analysis-based assay with PCR-RDB probe assay,thus,the concordance rate of the sample detection result was 99.1％ (447/451),and the concordance rate of the allele detection result was 99.6％ (898/902).DNA sequencing results of the 4 samples showed that 3 samples had the same genotyping result with melting curve analysis-based assay,and 1 sample had the same genotyping result with PCR-RDB probe assay.A rare β-globin mutation which was not included by melting curve analysis-based assay was not detected.Thus,the genotypes of 450 samples were detected accurately by melting curve analysis-based assay,and the concordance rate of the sample detection between the melting curve assay and DNA sequencing assay was 99.8％ (450/451).Among 84 fetal villi,amniotic fluid,and umbilical cord blood samples,8 mutation types and 18 genotypes were obtained by using melting curve analysis-based assay.All the samples have the same detection results by comparing melting curve analysis-based assay with PCR-RDB probe assay and DNA sequencing,so the concordance rate of the genotyping results was 100％ among the melting curve analysis-based assay,PCR-RDB probe assay and DNA sequencing.Conclusions The melting curve analysis-based PCR assay can detect multiple unknown samples simultaneously,and detect multiple mutations accurately.It is very useful for the genetic diagnosis and prenatal diagnosis of β-thalassemia.

Objectives Through analyzing the published scientific papers by the professionals of the Center for Disease Control and Prevention of Guangdong Province(GDCDC) from 2001 to 2006,to put forward some suggestions for the research management,the discipline development and the personnel training.Methods Literature quantitative analysis and health statistics methods were used to analyze these papers.Results The professionals of GDCDC published a total of 924 papers with an annual average of 154 in 114 kinds of periodicals from 2001 to 2006 of the 924 papers.264 papers(28.6%) were published in the key Chinese periodicals,320 papers(34.6%)in the South China Journal of Preventive Medicine,68 papers (7.4%)in the Chinese Journal of Health Laboratory Technology and 51 papers(5.5%)in the China Public Health.Of the 924 papers,433 papers(46.9%)belonged in the field of health laboratory technology,354 papers(38.3%)in the field of disease prevention and control,and 70 papers(7.6%)in the field of public health.During the six years,79.2% of the total professionals Published at least one paper,58professionals published at least six papers.Of the 924 corresponding authors.56.8% aged 30～40 years old.48.9% had a bachelor degree,and 45.9% had a senior professional title.Conclusions In recent years,the quantity and quality of papers published by the professionals of GDCDC were improved.The professional's ability to conduct scientific research was enhanced and the research level of GDCDC wag raised year by year.The predominant specialties of the academic research were in the field of health laboratory technology and disease prevention and control.But the professional work and discipline development of Public health should be sirengthened further.

BACKGROUND:More recently, studies have demonstrated that bone marrow mesenchymal stem cel s can be induced in vitro to differentiate into neuron-like cel s that are used for in vivo transplantation to repair nerve damage.
OBJECTIVE:To study the effect of bone marrow mesenchymal stem cel transplantation on learning and memory ability of senile dementia rats.
METHODS:Thirty male Sprague-Dawley rats were randomly divided into three groups:normal control group, stem cel therapy group and model control group. Rats in the latter two groups were used to establish animal models of senile dementia by intracranial injection ofβ-amyloid 1-40. Three weeks after modeling, rats were given bilateral hippocampal injection of induced bone marrow mesenchymal stem cel suspension in the stem cel therapy group, whereas no treatment was given in the normal control and model control groups. Morris water maze test was used to detect learning and memory ability of rats, and rat’s brain tissues were detected pathological y using hematoxylin-eosin staining.
RESULTS AND CONCLUSION:After modeling, the escape latency was higher and the cross-platform frequency was lower in the model control group compared with the normal control group. After cel transplantation, the escape latency and cross-platform frequency were gradual y shortened and increased with time, respectively. Compared with the model control group, the learning and memory abilities of rats were improved in the stem cel therapy group. The brain tissues were relatively intact in structure and exhibited less cel degeneration and necrosis in the stem cel therapy group compared with the model control group. To conclude, bone marrow mesenchymal stem cel transplantation exerts certain therapeutic effects on senile dementia by effectively improving the learning and memory ability.

OBJECTIVETo evaluate the clinical value of multicolor melting curve analysis(MMCA) for detecting genetic mutations in G6PD deficiency.

METHODSA total of 402 peripheral blood samples(256 males and 146 females) were collected from suspected patients or their relatives at the Prenatal Diagnosis Center of Liuzhou Maternal and Child Health Hospital between March 2012 and May 2012. The samples were screened by G6PD/6PGD quantitative ratio testing. The reliability of the assay was evaluated by multiplex probe melting curve assay(which can detect 16 G6PD mutations) and DNA sequencing through a double blind study.

RESULTSOne hundred seventy cases with G6PD/6PGD ratio < 1.0 and 232 cases with G6PD/6PGD ratio ≥ 1.0 were detected by the enzymological method. DNA sequencing has identified 182 wild type samples, 151 hemizygous mutation samples, 5 female homozygous mutation samples, 54 female heterozygous mutation samples and 10 female double heterozygous mutation samples. Multicolor melting curve analysis has detected 185 wild type samples, 148 hemizygous mutation samples, 5 female homozygous mutation samples, 55 female heterozygous mutation samples and 9 female double heterozygous mutation samples. The specificity and sensitivity of G6PD gene mutation detection by multicolor melting curve analysis were 100%(182/182) and 98.6%(217/220), respectively. The positive predictive value and negative predictive value were 99.5%(216/217) and 98.4%(182/185), respectively, and the Youden's index was 0.986. The concordance rate of the sample detection between the melting curve assay and DNA sequencing was 99.0%(398/402). Twenty-one different genotypes were detected by the multicolor melting curve analysis and 24 different genotypes were detected by DNA sequencing. Four samples containing mutations(c.196T>A or c.406C>T) were not detected by multicolor melting curve analysis, which can be attributed to different technical settings of the two methods.

CONCLUSIONMulticolor melting curve analysis for G6PD gene mutation detection is a simple, rapid, sensitive and specific method, which can be used for clinical diagnosis of G6PD deficiency.

Adolescent ; Adult ; Child ; Child, Preschool ; Female ; Glucosephosphate Dehydrogenase ; genetics ; Humans ; Infant ; Infant, Newborn ; Male ; Mutation ; Polymerase Chain Reaction ; methods ; Sequence Analysis, DNA

OBJECTIVE: To determine the composition and distribution of beta-thalassemia-associated genotypes in Liuzhou area of Guangxi, China. METHODS: From January to December 2017, 13 847 individuals who came for premarital examination, maternity examination or health check were recruited with informed consent. The subjects were analyzed by reverse dot blotting (RDB) for 17 common beta-thalassemia-associated variants among the Chinese population. Individuals with inconsistent results by blood test, electrophoresis, and RDB were subjected to Sanger sequencing to detect rare variants of the beta globin gene. RESULTS: In total 2098 individuals were found to harbor beta-thalassemia-associated variants, which included 2075 heterozygotes (98.90%), 12 compound heterozygotes (0.57%) and 11 homozygotes (0.52%). CD41-42 (48.43%) and CD17 (31.45%) were the most common variants. Three hundred and thirty eight-individuals were found to also carry heterozygous variants of the alpha globin gene, with the most common types being --SEA/aa, -a3.7/aa, aCSa/aa, -a4.2/aa. Through Sanger sequencing, rare genotypes such as beta-32/betaN, betaCD41-42/betaIVS-II-5 and betaCD30/betaN were detected. CONCLUSION Liuzhou area has a high incidence of beta-thalassemia, but with a complex variant spectrum and clinical phenotypes different from other regions. Genetic counseling and prenatal diagnosis for the carrier population is crucial for the reduction of the related birth defects. Our result may provide valuable information for the prevention and control of beta-thalassemia in this area.
China ; Female ; Genetic Counseling ; Genetic Variation ; Genotype ; Humans ; Mutation ; Pregnancy ; Prenatal Diagnosis ; alpha-Globins ; genetics ; beta-Globins ; genetics ; beta-Thalassemia ; diagnosis ; genetics