Objective To observe the infected instances of civil servant by Hp in Beijing and study the relationship between Hp and age,sex.Methods 3275 people were divided into four groups based on age:yong group,midlife group,pre-agedness group and agedness group;each group based on sex were divided into two groups.Every one was detected by 13C-urea breath test.If the resalt is over four,it is positive,otherwise it is negative.Measure dates were analised by statistical software.The infecting rate of Hp is denoted by persent,Chi square test was used in the every group.Results The mean infecting rate of Hp was 41.47%.The women in midlife group was 46.02%,it is the highest.The men over 75 in agedness group was 38.48%,it is the lowest.The infecting persent of yong group was higher than that of agedness group.The persent of midlife group and pre-agedness group was meaning result.The difference in age and sex had no statistical meaning.Conclusion The infecting rate of civil servant by Hp in Beijing is obviously lower than of people in Beijing,which is no relationship with sex and age.

OBJECTIVE:To establish a RP-HPLC method for the determination of the content of chlorhexidine acetate in daobian suppository.METHODS:The determination was performed on Diamonsil C_(18)column,The mobile phase consisted of acetonitrile-water-triethylamine(30:70:0.5,with pH adjusted to 3.0 by phosphoric acid)at a flow rate of 1.0 mL?min~(-1),The detective wavelength was 260 nm,the sample size was 20?L and the column temperature was maintained at 30℃. RESULTS:The linear range of Chlorhexidine Acetate was 1.092～13.10?g?mL~(-1)(r=0.999 9)and its average recovery was 98.29%(RSD=2.16%,n=9).CONCLUSIONS:The method is simple and accurate,and it can be used for the determination of the content of chlorhexidine acetate in daobian suppository.

Objective To investigate the relationship of hypothyroxinemia to gestational diabetes mellitus during early pregnancy. Methods A total of 11 365 cases of women with early singleton pregnancies were collected from Chongqing Health Center for Women and Children. The screening of thyroid function was performed. The postload glucose concentrations and the risks of gestational diabetes mellitus in hypothyroxinemia were investigated. The relationship of thyroid hormones to postload glucose concentration was evaluated. The association between thyroid peroxidase antibodies(TPOAb) and gestational diabetes mellitus was analyzed. Results Early pregnancy women with hypothyroxinemia had a higher postload glucose concentration. Comparing to normal pregnancy women, the prevalence of gestational diabetes mellitus in hypothyroxinemia was higher(42.2%vs18.4%, P<0.05). Free thyroxine(FT4) was significantly negatively correlated with postload glucose concentration. FT4 was associated with gestational diabetes mellitus. The risk of gestational diabetes mellitus was greater when FT4 was lower. The prevalence of gestational diabetes mellitus in early pregnancy women with TPOAb levels ≥500 IU/ml was significantly higher than that in normal pregnancy women (31.2%vs 18.4%, P<0.05). Conclusion Early pregnancy women with hypothyroxinemia and TPOAb≥500 IU/ml were related to the risk of gestational diabetes mellitus. Lower FT4 was the risk factor of gestational diabetes mellitus.

Objective To observe the effect of single intensive hyperbaric oxygenation (HBO) on cytochrome C and caspase-3 in rats af-ter permanent middle cerebral artery occlusion (MCAO) very early. Methods Forty-eight male Sprague-Dawley rats were subjected to per-manent MCAO model using the intraluminal suture method, and were divided into control group (n=24) and HBO group (n=24). The HBO group stayed in the hyperbaric cabin with a pressure of 0.2 MPa for 9 hours 3 hours after MCAO. They were measured with Garcia scores 3 hours, 13 hours and 24 hours after MCAO. Apoptosis cells of ischemic penumbra tissue were investigated with TUNEL 13 hours and 24 hours after MCAO, while the level of cytochrome C and caspase-3 were measured with ELISA. Results The Garcia scores increased 13 hours and 24 hours after MCAO in both groups, but there was no significant difference between groups (t<2.07, P>0.05). The apoptosis cells were found in both groups 13 hours and 24 hours after MCAO, and less in the HBO group than in the control group (t>6.57, P<0.01). The levels of cytochrome C and caspase-3 were less in the HBO group than in the control group 24 hours after MCAO (t>2.41, P<0.05). Conclusion A single intensive HBO in very early stage may improve neurological function after cerebral ischemia in rats, which may associ-ate with the inhibition of cytochrome C and caspase-3 to reduce cell apoptosis.

Objective To investigate effect of hyperbaric oxygen therapy (HBOT) on delayed encephalopathy after carbon monoxide poisoning (DEACMP).Methods This was a prospective random study of 60 patients with DEACMP admitted to Beijing Tiantan Hospital.Among them,32 constituted the HBOT group and 28 were controls.All of the patients in both groups were given drugs to improve microcirculation and rehabilitation treatment.Additionally,the patients in the HBOT group were given hyperbaric oxygen therapy.The Mini-Mental State Examination (MMSE),the Barthel index and an index of age-related white matter changes (ARWMC) were used assess the patients' cognition,motor function and cerebral white matter lesions on the day of enrollment and on the 35th and 70th day after treatment.Results Before treatment there was no significant difference in average MMSE,Barthel index or ARWMC scores between the groups.In the HBOT group the average MMSE and Barthel index scores on the 35th and 70th day after enrollment were significantly higher than on the day of enrollment and the average ARWMC score on the 70th day was significantly lower than at enrollment.On the 35th day the average MMSE and Barthel index scores of the HBOT group were significantly higher than those of the control group,but there was no significant difference in the groups' average ARWMC scores.On the 70th day after enrollment the HBOT group's average MMSE and Barthel index scores were still significantly higher than those of the control group,but its average ARWMC score was significantly lower.Conclusion HBOT can help improve cognitive and notor function and also alleviate cerebral white matter lesions of DEACMP patients.

Objective To investigate the roles of cardiac activity index(Tei index) on evaluation the right ventricular function after neonatal asphyxia.Methods Sixty neonatal asphyxia who included 35 cases of mild asphyxia(mild asphyxia group) and 25 cases of severe asphyxia(severe asphyxia group) and 30 cases of normal full-term newborns(control group) were selected.Echocardiographic examinations were performed on 24-48 h after birth,which included pulmonary artery systolic pressure (PASP),right ventricular ejection fraction(RVEF),tricuspid early diastolic peak(peak E) and late diastolic peak(peak A),and E/A ratio was acquired.The right ventricular cardiac activity index (RV-Tei index) was measured by Doppler spectrum.Results There was no significant difference in RVEF,E/A ratio among mild asphyxia group,severe asphyxia group and control group (P ＞ 0.05).RV-Tei index in mild asphyxia group and severe asphyxia group was increased compared with that in control group (0.489 ± 0.090,0.625 ± 0.100 vs.0.345 ± 0.120),and there was significant difference (P＜ 0.05 or ＜0.01).There was significant difference in RV-Tei index between mild asphyxia group and severe asphyxia group (P ＜ 0.05).RV-Tei index in neonatal asphyxia was positively correlated with PASP (r =0.950,P ＜ 0.05),and there was no relationship between RV-Tei index and gestational age,weight,heart rate (r =-0.068,-0.280,-0.360,P ＞0.05).Conclusions Neonatal asphyxia can lead to disorders of the right ventricular function.Tei index can evaluate early overall changes of the right ventricular function and is better than conventional ultrasound technology in neonatal asphyxia.

Objective To investigate the effect of early hyperbaric oxygen on traumatic basal ganglia infarction in infants and children.Methods 93 cases with basal ganglia infarction after cerebral trauma were reviewed. They were divided into hyperbaric oxygen group (n=60) and control group (n=33). Results 100% of the hyperbaric oxygen group improved, and 93.33% were cured; it was 81.82% and 75.76%in the control group (P<0.05). Conclusion Early hyperbaric oxygen is safe and effective on traumatic basal ganglia infarction in infants andchildren.

Objective To evaluate the efficacy of two doses of hyperbaric oxygen therapy (HBOT) on delayed encephalopathy after carbonmonoxide poisoning (DEACMP). Methods 68 patients with DEACMP were divided into two groups, the 30 sessions of HBOT group(30-HBOT group) and the 60 sessions of HBOT group (60-HBOT group), who were given HBOT 30 and 60 times respectively. All patientswere assessed with Mini-Mental State Examination (MMSE) and fluid attenuated inversion recovery (Flair) magnetic resonance imaging ofbrain immediately before, 35 d and 70 d after HBOT. Results The MMSE score was significantly higher in 60-HBOT group than in30-HBOT group 70 d after HBOT (P<0.01). All of patients showed diffuse and confluent hyper-intensity over the bilateral periventricularwhite matter and centrum semiovale on conventional T2WI and Flair, which improved. Conclusion HBOT is effective on DEACMP, especiallywith 60 times.

OBJECTIVETo investigate the eligibility and main problems for occupational health inspection agencies in China, and to provide technical references for improvement of occupational health inspection.

METHODSA survey was performed in occupational health inspection agencies that obtained eligibility before June 2011 in eight provinces: Zhejiang, Jiangsu, Shandong, Hubei, Guangxi, Shanghai, Chongqing, and Shenzhen. The survey used the General Information Questionnaire for Occupational Health Inspection Agency made by the project of Occupational Health Surveillance and Diagnosis and Identification of Occupational Diseases in China and Australia.

RESULTSA total of 650 agencies obtained eligibility for occupational health inspection in the eight provinces. These agencies contained 343 centers of disease control and prevention (CDC) or health and epidemic prevention stations (52.8%), 219 hospitals (33.7%), 25 institutes or centers for occupational disease prevention and control (3.8%), 29 community health service centers (4.5%), and 34 other agencies (5.2%) including departments of preventive and health care and preventive medicine outpatient departments. Four hundred and fifty-three agencies completed the questionnaire survey with a response rate of 69.7%. The main types of eligible agencies were different among various regions. A majority of occupational health inspection agencies were hospitals in Shanghai and Zhejiang (67.1%, 62.3%), departments of preventive and health care in Shenzhen (70.0%), and CDCs in Chongqing, Jiangsu, Hubei, and Guangxi. Each agency obtained 3.5 occupational health inspection eligibilities on average. Most of agencies could perform eligible health inspection for dust, harmful physical factors, or harmful chemical factors (84.8%, 87.9%, 87.2%). Moreover, 72.8% of agencies were eligible for all the three types of inspections. A few agencies were able to perform eligible health inspection for harmful biological factors or radiation work (22.5%, 23.0%).

CONCLUSIONAn occupational health inspection network has been established in these surveyed regions. CDCs, institutes for occupational disease prevention and control, and hospitals are the main agencies for occupational health inspection. In order to build up a network of occupational disease prevention and control, various agencies should make full use of their own advantages and enhance abilities and technical cooperation.

China ; Hospitals ; Humans ; Occupational Diseases ; epidemiology ; Occupational Health ; Occupational Health Services ; organization & administration ; Organizations ; Surveys and Questionnaires

Objective To establish a method for determination of dichloromethane in drinking water by purge and trap gas chro‐matography‐mass spectrometry .Methods Dichloromethane in drinking water was purged and trapped ,then desorbed and detected by using gas chromatography‐mass spectrometry .Quantitive analysis was conducted by using standard curve under selected ion mo‐nitoring (SIM) mode .Results The operation of this method was simple ,and its limit of detection (LOD) was 0 .005 ng/mL .The recovery rate was higher than 97% ,and the relative standard deviation was less than 5% .Conclusion The method may be appro‐priate to measure dichloromethane in drinking water .