Objective The antibiotics resistance of common bacteria in orthopedics infection was investigated to guide rational application of antibiotics in treating infection disease.Methods Totally 500 secretions samples from orthopedic inpatients between June 2005 and June 2007 were cultured aerobically.The results of bacterial culture,antibiotics resistance of common bacteria and antibiotics application were analyzed.Results 43 kinds,248 strains of pathogenic bacteria were detected,and 52.8% of them were Gram-negative bacteria.The result of drug sensitive tests indicates that resistance of common antibiotics has been increasing.Conclusion Gram-negative bacilli dominate the pathogenic bacteria in orthopedics patients and it tends to increase;while incidence of infections by Gram-positive cocci is lower.Some pathogenic strains acquire higher resistance against those antibiotics which were effective in the past.Antibiotics application in clinical practice is far from rational,and the irrationality is characterized by high antibiotics use rate,high star point,prolonged postoperative medication,excessive drug combination etc.