1.Study on the in vitro Cell Uptake and Toxicity of Resibufogenin-loaded PLGA-TPGS Nanoparticles
Hong XU ; Meng GAO ; Qiuchen CHU ; Hao DONG ; Yu CHEN ; Rongqian XU ; Chenghong ZHANG ; Yan TIAN
China Pharmacy 2017;28(16):2252-2255
OBJECTIVE:To study the in vitro uptake of Resibufogenin(RBG)lactic acid glycolic acid copolymer-water solu-ble vitamin E (PLGA-TPGS) in human liver cancer HepG2 cells,mouse ascites-type lymphatic metastasis of tumor HCa-F cells, and the toxicity on HepG2 cells. METHODS:RCPTN loading RBG and coumarin-6(C6)were prepared. Fluorescent inverted mi-croscope was used to observe the in vitro uptake by RCPTN HepG2,HCa-F cells. It was divided into negative control group,blank PLGA-TPGS nanoparticles(EPTN)group,5-fluorouracil solution(FS)group,RBG solution(RS)group,RBG/PLGA nanoparti-cles(RPN)group and RPTN group. WST-1 was conducted to investigate the optical density at 450 nm wavelength of HepG2 cells after 24,48,72 h incubated by FS,RS,RPN and RPTN with different final concentrations (1.25,2.5,5,10,20 μg/mL);the cell viability (CV) and half inhibitory concentration (IC50) were calculated. RESULTS:RCPTN distributed around the nucleus of HepG2,HCa-F cells. CV was decreased by RBG concentration increased in RPN group and RPTN group,and decreased by time prolonged;compared with FS group,CV in RPTN group was decreased(P<0.05 or P<0.01). IC50 of HepG2 cells incubated by FS,RS,RPN and RPTN was decreased by time prolonged,ordered by RS>FS>RPN>RPTN;IC50 incubated by RPN and RPTN for 48,72 h was obviously less than that of FS and RS(P<0.05 or P<0.01). CONCLUSIONS:RPTN can deliver RBG in-to HepG2,HCa-F cells,showing inhibition effect on HepG2 cells which is stronger than RPN,RS and FS.
2.Operation and Administration of Rehabilitation Institutions in China
Zhongxiang MI ; Qiuchen HUANG ; Fei LIU ; Jianjun LI ; Jun CHENG ; Zhiru CUI ; Pei LI ; Haifeng SHI ; Hao DONG ; Ailing ZHU ; Baohua WANG ; Xiaoyue ZHOU ; Wen BAO ; Didi LU ; Ying LI ; Tianyi CHU ; Mengmeng WANG
Chinese Journal of Rehabilitation Theory and Practice 2015;(1):103-105
How to give full play to the advantages of rehabilitation institutions, and to improve service quality and administration efficiency are some of the complex issues faced by the high-level administrators of rehabilitation institutions. This paper studied a total of 67 institutions to investigate the operation and management model of rehabilitation institutions in China, as well as their advantages and disadvantages.
3.Efficient Performance Management System of Medical Rehabilitation in China
Jun CHENG ; Zhongxiang MI ; Zhiru CUI ; Jianjun LI ; Pei LI ; Haifeng SHI ; Hao DONG ; Ailing ZHU ; Baohua WANG ; Xiaoyue ZHOU ; Wen BAO ; Qiuchen HUANG ; Fei LIU ; Ying LI ; Didi LU ; Tianyi CHU ; Mengmeng WANG
Chinese Journal of Rehabilitation Theory and Practice 2015;(1):106-108
The establishment of an efficient performance management system is crucial for a rehabilitation institution. This article discussed the concept of performance, performance management and ways to establish an efficient performance management system.