On the clinic,the main treatment of autoimmune diseases were used immunosuppressant,but large-scale use of immunosuppressive agents could cause of the delayed dysfunction,side effects such as infection,tumors and so on,so-induced immune suppression and immune tolerance response was the most effective way.So far,dendritic cells(DC) was the most powerful professional antigen-presenting cells.On one hand,DC as a full-time antigen-presenting cells and trigger regulation of the inherent and acquired immune response,start the immune response;On the other hand,DC through a variety of mechanisms induced antigen-specific T cells incompetent,show strong plasticity in immune tolerance and play an important role in immune regulation.Nuclear factor(NF)-?B is a nuclear transcription factor.In the immune response,NF-?B in the dendritic cells regulation of their own including development,maturity,the antigen-presenting,to stimulate T cells functions and so on.Such adjustments play an important role in the incidence of autommine diseases,could became a new target treatment of autommine diseases.

Objective To explore the influence of salmeterol fluticasone plus tiotropium bromide on right cardiac and thyroid functions of patients of chronic obstructive pulmonary disease ( COPD) with chronic cor pulmonale .Methods A total of 92 cases of COPD patients with chronic cor pulmonale at our hospital from July to December 2012 were recruited as subjects and divided randomly into 2 groups of treatment ( n=47) and control (n=45).The control group received diuretics and vasodilative drugs while the treatment group had additional therapies of salmeterol fluticasone ( 50/500 μg ) twice daily plus tiotropium bromide once per day once daily for 6 months.Measure and compare two groups of patients′FEV1%, forced expiratory volume in one second(FEV1), status of vigor, brain natriuretic peptide(BNP), left ventricular ejection fraction ( LVEF ) , ultrasonic cardiogram ( right ventricular outflow tract , right ventricular internal diameter, right pulmonary artery trunk ) and value of thyroid function before and after treatment .Results Before treatment, the treatment group′s FEV1 (%) (36 ±10)%, FEV1 (1 036 ±410) ml, BNP (357 ± 101) ng/L, LVEF (46.2 ±10.4)%, internal diameter of right ventricular outflow tract (36.0 ±5.4) mm, right ventricular internal diameter (38.1 ±6.7) mm, right pulmonary artery trunk (24.0 ±3.6) mm, TT3 (1.47 ±0.38) nmol/L, TT4 (114.56 ±21.39) nmol/L, FT3 (4.47 ±1.42) pmol/L, FT4 (15.6 ± 2.5) pmol/L and TSH (2.50 ±0.91) mU/L showed no marked difference from those of control group (P>0.05).After treatment, the treatment group′s FEV1(%)(58 ±1)%, FEV1(1 706 ±456)ml, BNP(225 ± 52) ng/L, LVEF (57.1 ±11.5)%, internal diameter of right ventricular outflow tract (30.1 ±4.2) mm, right ventricular internal diameter ( 32.0 ±3.9 ) mm and right pulmonary artery trunk ( 20.2 ±2.3 ) mm greatly improved ( P <0.01 ) while its TT3 ( 1.46 ±0.42 ) nmol/L, TT4 ( 113 ±22 ) nmol/L, FT3 (4.46 ±1.38) pmol/L, FT4 (15.4 ±2.5) pmol/L and TSH (2.51 ±0.82) mU/L showed no marked difference from those of control group ( P>0.05 ) .However , no obvious difference existed in the control group′s BNP, LVEF, internal diameter of right ventricular outflow tract , right ventricular internal diameter , right pulmonary artery trunk and value of thyroid function before and after treatment ( P >0.05 ) . Conclusion Salmeterol fluticasone plus tiotropium bromide can effectively improve right cardiac functions of patients of COPD with chronic cor pulmonale , without marked suppression of thyroid function .

Objective To evaluate the curative effects of the modified fixed reverse Twin-block ap-pliance (TBA)combined Edgewise in the treatment of adult Angle Ⅲ anterior crossbite. Methods The appliance was used in 18 subjects with adult Angle Ⅲ skeletal malocclusion and clinical characters and X-ray cephalometry were then compared before and after treatment. Results The mandibular soft tissue changes could be achieved fast and the profile had changed effectively. Moderate labioclination of maxil-lary anterior teeth played a role in the improvement of axillary soft tissue protrusion. The cases were fol-lowed up as long as 3 years. No relapse occurred in all the patients. Anterior occlusion was normal. Con-clusion All findings indicate that adult Angle Ⅲ anterior crossbite can be quickly corrected with the mod-ified fixed reverse TBA combined Edgewise. The effect on mandibular soft tissue is significant.

Objective:To study the distribution of allergens in our district and the correlation between skin prick test with antigen and nasal provoking test in patients with allergic rhinitis.Method:A total of 1 400 cases with allergic symptoms and signs underwent skin prick test with standardized aeroantigens provoking by AllergoPhamar Company. The patients allergic to Dermatophagoides with different scale of the skin prick test were taken nasal provoking test in order to evaluate the correlation between skin prick test and nasal provoking test.Result:One thousand and eighty-eight(78%) cases showed a positive result in skin prick test.The percent of patients with positive skin prick test were as follows: 462 cases of Dermatophagoides pleronyssinus, 428 cases of Dermatophagoides farinae. Positive perennial aeroantigens were 52.2%(568),positive seasonal aeroantigens were 35.7%(388). All the patients of the scale of skin prick test was +++ and ++++ were positive. There are 29 patients were positive with nasal provoking test which the scale of skin prick test was ++. There are 21 patients were positive with nasal provoking test,which the scale of skin prick test was +.Conclusion:The correlation between skin test with antigens and nasal provoking test in patients whose positive scale were +++ and ++++. There are false positive in the patients whose positive scale were + and ++. The specific immunotherapy should consider the scale of the skin prick test and eliminate the false positive before the therpy.

[Objective] To observe the sera immunoglobulin E(IgE) and Complement C3(C3)level changes in patients with chronic urticaria.[Methods] To detect sera IgE and Complement C3 content of 60 cases of chronic urticaria patients and 60 cases of healthy persons by chemiluminescence and Immunity Transmission Turbidity.At the same time,we tested sera IgE and Complement C3 content of both penetration needling and drug groups before and after treatment of 60 cases,and anaylysed the correlation of IgE and Complement C3.[Results]Higher level of sera IgE was detected in patients with chronic urticaria(P 0.05);the correlation of IgE and Complement C3 was negative in both groups.[Conclusions]Detecting Sera IgE and Complement C3 level have reference value in the diagnosis and treatment of chronic urticaria.

Since cardiopulmonary resuscitation(CPR)guidelines was constituted in 1974,it was adopted many times.The American Heart Association,in collaboration with the International Liaison Committee on Resuscitation(ILCOR),adopted new CPR science guidelines in January,2005.This article gives a short introduction of CPR,and showes the progress of several editions.

ObjectiveTo explore the mental health level of family members of stroke patient and related factors.Methods34 stroke patients' family members were tested with the Symptom Checklist-90(SCL-90), and compared with norms; SCL-90 factor scores, which were higher than that of norms, were analyzed for related factors.ResultsScores of 4 factors in the SCL-90 (somatization, depression, anxiety and phobia) were higher than norms in the family members of stroke patients. Scores of somatization factor correlated with the education background; depression factor correlated with the family income variable; anxiety factor correlated with the cognitive impairment variable of patients. In the correlation analysis with patient's activities of daily living (ADL) scores, somatization factor negatively correlated with total score of ADL and hygiene, feeding, bowel management, locomotion, bathing scores; both depression factor scores and positive item numbers negatively correlated with feeding and bowel management scores; phobia anxiety factor scores with hygiene, feeding, bowel management, communication, locomotion, bathing and total scores.ConclusionThe mental health level of family members of stroke patient is lower, and it is related to family income reduction, patient's cognitive impairment, and especially with the patient\s ADL level.

BACKGROUND:Surface marker molecules and differential ability of human adipose tissues-derived mesenchymal stem cells can change with the prolonged culture time.OBJECTIVE:To analyze the morphological characteristics and colony forming capacity of early cultured rabbit adipose tissues-derived mesenchymal stem cells in vitro.DESIGN,TIME AND SETTING:The cytological in vitro study was performed at the Institute of Ophthalmology,and Central Laboratory,Eye and ENT Hospital,Fudan University from January to March 2008.MATERIALS:Nine female New Zealand rabbits aged three months were obtained from the Shanghai Yingenyang Rabbit Room.METHODS:Adipose tissue was harvested from the nape fat pad of the New Zealand rabbits following anesthesia.Primary cultured cells were established using type I collagenase and cell cultures were maintained with DMEM containing 0.1 volume fraction of fetal bovine serum.Cells were passaged when 80% was confluent.The second,third and fourth passages of cells were utilized for the study.MAIN OUTCOME MEASURES:The morphology of adipose tissues-derived mesenchymal stem cells was observed using inverted microscope.Cell phenotype was detected utilizing flow cytometry.The cloning capacity was determined.RESULTS:Adipose tissues-derived mesenchymal stem cells were fibroblast-like and proliferated rapidly in vitro at the second,third and fourth passage.Flow cytometry showed that adipose tissues-derived mesenchymal stem cells expressed CD29 and PCNA.The colony-forming rates were respectively(8.0?0.6)%,(6.7?0.4)% and(4.6?0.5)%(F=12.18,P