Objective To investigate the infection effects of different strains(Prototype,Delta,Beta,and Omicron BA. 1)of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)on transgenic mice K18-hACE2 expressing angiotensinconverting enzyme 2(ACE2)by nasal drip.MethodsMice were challenged with each strain of different gradients of doses by nasal drip,which were monitored for body mass and survival daily for 6 d in Beta group and 14 d in the other three groups. The median lethal dose(LD_(50))was calculated by IBM SPSS Statistics 26 analysis software,and the virulence of different SARS-CoV-2 strains were compared by detection of the viral nucleic acid loads of lung,brain and turbinate tissues and the lesion of lung tissues.ResultsBeta strain showed the strongest virulence and the highest lethality,and the lowest dose(1 CCID_(50))was 100% lethal with a LD_(50)less than 1 CCID_(50);The LD_(50) of Delta,Prototype and Omicron BA.1 strains were 0. 52,0. 60 and 52. 92 CCID50respectively. The copy number of viral nucleic acid in lung tissue of mice infected with different strains elevated with the increase of challenge dose,and the copy number of viral nucleic acid in brain tissue of dead mice reached 10~8～ 10~(11) copies/mL. The lung tissues of mice infected with different strains of different doses showed different degrees of pathological damage.ConclusionThe virulence of different SARS-CoV-2 strains in K18-hACE2 mice from high to low were Beta,Delta,Prototype and Omicron BA.1.

The clinical data of 16 patients with synovitis,acne,pustulosis,hypemstosis,osteitis (SAPHO) syndrome were retrospectively analyzed.There were 7 men and 9 women with a mean age of (38 ± 11) years at onset and middle-aged females predominated.The most common clinical manifestations were palmoplantar pustulosis and osteoarticular disease involving anterior chest wall,spondylarthrilides and shoulder joint.ECT was the sensitive imaging examination for SAPHO syndrome.Combined treatment can alleviate symptoms of the disease.

Depressed syndrome theory in Chinese Medicine originated from Huang Di Nei Jing and was developed and consummated by later doctors.Since the pathological changes and treatment of five essences depressed syndrome were ehcidated in Huang Di Nei Jing,doctors from successive generations had continuously been tmprovmg and ennclung the academic essential of depressed syndrome theory in Chinese Medicine,which made the theory more mature and flawless.All the theories,including Bing Wang'S improvement in the theory of treatment in five essences depressed syndrome,the first time Wuze Chen pointing out emotion leading to depressed syndrome,Qi-Blood-Flow-Smoothly theory raised by Congzheng zhang,the opinion of six depression by Zhenheng Zhu,the development on the theory of treatment in five essences depressed svndrome by Lv Wang,the posed of Abnomal-Rising-and-Failing-of-Vital-Energy by Sigong Dai,Lun Wang's version of relationship of Qi,Blood and phlegmatic stagnation,the idea from Xianke Zhao of all depressed syndrome belonging to liver disease,Yikui Sun'S talking about Qi itself in five viscera is depressive,and Shixiong Wang'S discussion about the most important character of Qi in body is flowing,gradually guided the research on depressed syndrome theory in Chinese Medicine moving forward.In this paper,the author emphasizes the development of depressed syndrome theory in Chinese Medicine.

The main content of TCM emotional therapy is according to the pathogenesis of abnormal emotional changes, such as functional disorder of viscera, qi and blood, corresponding therapeutic methods are formulated for the purpose of regulating mental state, eliminating emotional stimulating, restoring balance of viscera, qi and blood, and treating diseases in both body and mind. This paper outlined the emotional therapy in Chinese Medicine, demonstrated that TCM psychology has a long history, rich contents and variety of methods.

Objective To evaluate the effect of pelvic floor muscle training in postpartum urinary incontinence. Methods A total of 355 postpartum women 6 weeks after delivery were randomly allocated to training group ( 182 cases) and control group ( 173 cases). The training group attended in one to one pelvic floor muscle training conduction. The control group received the customary information. The two groups finished the questionaire 6 months after delivery to understand the general state of health, duration of pregnancy, urinary tract symptom and urinary incontinence 6 weeks,6 months after delivery. Results Ten cases failed, 4 cases lost in the training group and 6 cases lost in the control group. Significantly fewer women in the training group were reported stress urinary incontinence compared with the control group 6 months after delivery [11.3%(19/168) vs. 21.0%(35/167)](P＜0.01). Significantly fewer frequency of nocturia in the training group was reported compared with the control group 6 months after delivery [(1.3 ± 0.3) times vs.(2.2 ± 0.4) times ] (P ＜ 0.01 ). Conclusion Pelvic floor muscle training plays a positive role in postpartum urinary incontinence.

Objective In this research with the method of Alu -PCR we investigate the integration of hepatitis B virus ( HBV) DNA in human hepatocarcinoma cell line (PLC/PRF/5) genome DNA.Methods We at first extracted the genome DNA from PLC/PRF/5 cells, and then the potential integration fragments of HBV DNA and human genome DNA were amplified with according to the Alu -PCR after three rounds PCR .The Alu-PCR amplification products were observated with agarose gel electrophoresis , then integration positive electrophoresis bandings were chipped and purified for nucleic acid sequencing .At last he bioinformatics information was acquired by blast online.Results Through agarose gel electrophoresis after Alu -PCR amplification, we got four potential integration bindings , among which we got three integration sequences of HBV DNA in human genome DNA .These integration sequences could be individually located in the human chromosome of 03p21.31, 05p15.33, 12q13 and 12-q14.1.Conclusion With Alu-PCR we can accurately measure the integration of HBV DNA in human genome DNA , and Alu-PCR can be a a convenience and economic method in the study of HBV DNA ′s integration in human genome DNA .

Tissue factor (TF), a transmembrane glycoprotein with a molecular mass of 47000, plays an important role of coagulation within vessel wall. Recently, people also present that TF have identifying and regulating function in cells signaling. And it contributes in the pathogenesis of atherosclerosis, angiogenesis and restenosis. This review presents the development of TF in above mentioned .

Objective: To observe the change of the levels of tumor necrosis factor-?(TNF-?),interferon-?(IFN-?) and interleukin-10(IL-10) in bronchial alveolar lavage fluid(BALF) from rats with pulmonary fibrosis induced by bleomycin and investigate the intervention by IFN-? on it. Methods: The SD rats were randomly divided into three groups: the normal control group(Group N),the bleomycin-A_5 group(Group B),and the interferon-? treated group(Group I).The pulmonary fibrosis model was established by a single intratracheal injection of 5 mg/kg of bleomycin.The rats were sacrificed at day 17,21,28 after modeling in batch.Semi-quantitative analysis of the histopathologic changes stained with Haematoxylin-eosin(H-E) was performed.The levels of TNF-?,IFN-? and IL-10 in BALF were measured with ELISA.The content of hydroxyproline in the lungs was determined by digestion method.Results:①The scores of pulmonary alveolitis and pulmonary fibrosis in Group I and Group B were significantly higher from 17d after bleomycin administration compared with Group N(all P

On the basis of the research on interaction between plasmodium and mosquito vector,the mechanism of innate immune defense responses in anopheline mosquitoes against plasmodium infection has been studied.The innate immune defense may be applied to confine and kill malaria parasites under migration and development,and contribute to an effective control strategy on malaria vectors.

Objective To explore the association of single nucleotide polymorphism-SNP44 of Calpain 10 gene with genetic susceptibility to type 2 diabetes in Kunming.Methods The polymorphism of site UCSNP44(T/C) in Calpain 10 gene was detected by Polymerase chain reaction and Allele specific amplification(PCR-ASA)in 131 type 2 diabetic patients and 131 non-diabetic controls who were selected using case-control study in the Kunming urban communities.Results The frequency of"C"allele and"TC"gene of SNP44 in type 2 diabetic patients was significantly higher than that in the controls.Conclusion The study suggested that the polymorphism-SNP44 of Calpain10 gene may be associated with genetic susceptibility to type 2 diabetes in Kunming population.