Objective To investigate the infection effects of different strains(Prototype,Delta,Beta,and Omicron BA. 1)of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)on transgenic mice K18-hACE2 expressing angiotensinconverting enzyme 2(ACE2)by nasal drip.MethodsMice were challenged with each strain of different gradients of doses by nasal drip,which were monitored for body mass and survival daily for 6 d in Beta group and 14 d in the other three groups. The median lethal dose(LD_(50))was calculated by IBM SPSS Statistics 26 analysis software,and the virulence of different SARS-CoV-2 strains were compared by detection of the viral nucleic acid loads of lung,brain and turbinate tissues and the lesion of lung tissues.ResultsBeta strain showed the strongest virulence and the highest lethality,and the lowest dose(1 CCID_(50))was 100% lethal with a LD_(50)less than 1 CCID_(50);The LD_(50) of Delta,Prototype and Omicron BA.1 strains were 0. 52,0. 60 and 52. 92 CCID50respectively. The copy number of viral nucleic acid in lung tissue of mice infected with different strains elevated with the increase of challenge dose,and the copy number of viral nucleic acid in brain tissue of dead mice reached 10~8～ 10~(11) copies/mL. The lung tissues of mice infected with different strains of different doses showed different degrees of pathological damage.ConclusionThe virulence of different SARS-CoV-2 strains in K18-hACE2 mice from high to low were Beta,Delta,Prototype and Omicron BA.1.

Decompression, Surgical ; Humans ; Nose ; surgery ; Ophthalmologic Surgical Procedures ; methods ; Orbit ; surgery

Objective: To study the determinants that affect patient satisfaction to rural doctors. Methods:Based on survey data of Liupanshui City, Guizhou Province, structural equation modeling analysis was adopted, and the study was mainly from the following four aspects:the image of rural doctors, service quality, quality of diagnosis and treatment, and harmonious doctor-patient relationships. Results: The study found that the quality of diagnosis and treatment had the greatest direct impact on rural patient satisfaction, followed by service quality, the image of ru-ral doctors and harmonious doctor-patient relationships;rural patient satisfaction has a significant impact on rural pa-tient loyalty. Conclusion:Rural patient satisfaction and loyalty to rural doctors is mainly based on kinship and geopo-litical relations;given the state of medical technology, the medical environment, medical equipment, service aware-ness and other restrictions, rural doctors cannot fulfill the “gatekeeper” role, and consequently, the primary health-care system has been severely affected.

Objective To investigate the value of early hematocrit (Hct) level in predicting early neurological deterioration (END) in patients with acute ischemic stroke. Methods The patients with acute ischemic stroke within 24 h of onset were enrolled prospectively. They were divided into low Hct, normal Hct, and high Hct according to the quantile of the measured Hct. END was defined as an increase of ≥2 of the National Institute of Health Stroke Scale (NIHSS) score or ≥1 of the motor item score within 5 d after admission compared with the baseline. The vascular risk factors, clinical features, baseline NIHSS score, infarct size, and laboratory test variables were compared between the END group and the non -END group. Multivariate logistic regression analysis was used to identify the independent risk factors for END. Results A total of 216 patients with acute ischemic stroke were enrolled, including 128 males (59.26%). Their mean age was 67.40 ±14.12 years. Sixty-two patients (28.70%) experienced END. The normal ranges of Hct in male and female were 40.12%-46.35% and 38.32%-44.17%, respectively. Univariate analysis indicated that there were significantly differences in baseline NIHSS score ( P =0.001), fasting glucose (P =0.030), C reactive protein (CRP) (P =0.041), and the proportions of different Hct levels between the END group and the non-END group (P =0.023). The END incidences in patients with high -level Hct (40.0%) and low –level Hct (35.2%) were significantly higher than that in the normal Hct patients (20 .4%), but there was no significant difference between the high-level and low -level Hct patients ( P = 0.690). Multivariate logistic regression analysis showed that theigh-level Hct (odds ratio 2.460, 95% confidence interval 1.146-5.283; P =0.021) and the baseline NIHSS score (odds ratio 1.070, 95% confidence interval 1.014-1.129; P = 0.013) were the independent risk factors for END. Conclusion The elevated Hct in patients with acute ischemic stroke are susceptible to END.

The clinical data of 16 patients with synovitis,acne,pustulosis,hypemstosis,osteitis (SAPHO) syndrome were retrospectively analyzed.There were 7 men and 9 women with a mean age of (38 ± 11) years at onset and middle-aged females predominated.The most common clinical manifestations were palmoplantar pustulosis and osteoarticular disease involving anterior chest wall,spondylarthrilides and shoulder joint.ECT was the sensitive imaging examination for SAPHO syndrome.Combined treatment can alleviate symptoms of the disease.

Tissue factor (TF), a transmembrane glycoprotein with a molecular mass of 47000, plays an important role of coagulation within vessel wall. Recently, people also present that TF have identifying and regulating function in cells signaling. And it contributes in the pathogenesis of atherosclerosis, angiogenesis and restenosis. This review presents the development of TF in above mentioned .

OBJECTIVE To explore the value of molecular epidemic technology on tuberculosis outbreak in prison.METHODS Using mycobacterial interspersed repetitive unit(MIRU) genotyping to discriminate and recognize 7 strains on tuberculosis cases.RESULTS Among 7 Mycobacterium tuberculosis isolates,6 strains′ MIRU genotyping was 233325153533,another one was 242325152322.Six M.tuberculosis cases were infected by the same MIRU genotyping tuberculosis strains,but another one was not.CONCLUSIONS MIRU genotyping to discriminate and track source of infection is possible.

Objective: To observe the change of the levels of tumor necrosis factor-?(TNF-?),interferon-?(IFN-?) and interleukin-10(IL-10) in bronchial alveolar lavage fluid(BALF) from rats with pulmonary fibrosis induced by bleomycin and investigate the intervention by IFN-? on it. Methods: The SD rats were randomly divided into three groups: the normal control group(Group N),the bleomycin-A_5 group(Group B),and the interferon-? treated group(Group I).The pulmonary fibrosis model was established by a single intratracheal injection of 5 mg/kg of bleomycin.The rats were sacrificed at day 17,21,28 after modeling in batch.Semi-quantitative analysis of the histopathologic changes stained with Haematoxylin-eosin(H-E) was performed.The levels of TNF-?,IFN-? and IL-10 in BALF were measured with ELISA.The content of hydroxyproline in the lungs was determined by digestion method.Results:①The scores of pulmonary alveolitis and pulmonary fibrosis in Group I and Group B were significantly higher from 17d after bleomycin administration compared with Group N(all P

On the basis of the research on interaction between plasmodium and mosquito vector,the mechanism of innate immune defense responses in anopheline mosquitoes against plasmodium infection has been studied.The innate immune defense may be applied to confine and kill malaria parasites under migration and development,and contribute to an effective control strategy on malaria vectors.

Objective To explore the association of single nucleotide polymorphism-SNP44 of Calpain 10 gene with genetic susceptibility to type 2 diabetes in Kunming.Methods The polymorphism of site UCSNP44(T/C) in Calpain 10 gene was detected by Polymerase chain reaction and Allele specific amplification(PCR-ASA)in 131 type 2 diabetic patients and 131 non-diabetic controls who were selected using case-control study in the Kunming urban communities.Results The frequency of"C"allele and"TC"gene of SNP44 in type 2 diabetic patients was significantly higher than that in the controls.Conclusion The study suggested that the polymorphism-SNP44 of Calpain10 gene may be associated with genetic susceptibility to type 2 diabetes in Kunming population.