Liver metastases is the independent risk factor of prognosis of colorectal cancer.In this article we summarized the research about the mechanism of colorectal liver metastases over the latest five years.Studies showed that the specific cell adhesive interaction in metastatic target organs plays an important role in metastases. The metastatic procedure call be roughly assigned to:escape,adhesion,invasion,survival and proliferation.The interactions between tumor cells and nonparenchymal cens in the liver lead to tumor growth.Tumor micro-environ ment is characterized by the lack of oxygen,inflammation,immune suppression,meanwhile,the role played by cell factors in the tumor micro-environment can not be ignored.

Objective To study the clinical nursing significance of ECG monitor in primary PCI for AMI patients and the relation of changes in ECG and the reperfusion of infarction related artery.Methods Regressional analysis was made in ECG changes including different types of arrthymia,degree of ST segment regression,and their relation between the reperfusion of infarction related artery before and after interventional therapy in AMI patients.Results In 468 AMI patients,142 patients had ventricular arrhythmia before therapy.After interrentional therapy,285 patients were co-morbid with accelerated ventricular tachycardia,203 patients with transient ventricular tachycardia or R-on-T phenomenon,62 patients with ventricular fibrillation and received asynchronous electriacal cardioversion.Twenty four patients had new onsent of Ⅱ-Ⅲ degree atrio-ventricular block and 45 patients had bradycardia or sinus arrest.The atrio-ventricular blook in 10 patients before PCI disappeared after the operation.There are 301 patients with apparent ST segment regression(ST segment regressed ≥50%,57 patients with partial ST segment regression,and 29 patients without ST segment changes.Conclusion Arrhythmias always accompanied with IRA reperfusion.Careful monitoring of ECG is essential for patients' safety after AMI.Obvious ST segment is observed in sufficient IRA reperfusion.However,poor regression of ST segment as well as haemodynamics disorder and cardiogenic shock is observed in patients with poor reperfusion or subacute occlusion of IRA.

0 05) between the two procedures in incidence of anastomotic leakage (25 0% vs 10 7%), pulmonary infection rate (31 3% vs 32 1%), incide nce of thoracic cavity infection (0 vs 3 6%), proportion of re-thoracotomy (0 vs 3 6%), incidence of d elayed gastric emptying (3 1% vs 21 4%), postoperative SICU stay (4d?2 3d vs 5d ?3 6d), and peri-operative mortality (3 1% vs 7 1%). The incidence of recurr ent laryngeal nerve injuries (28 1% vs 7 1%, ? 2=4 391,P =0 036) and arr hythmia (43 8 % vs 17 9%, ? 2 =4 627, P =0 031) in the THE Group were significantly higher than those in the TTE Group, while the intra-operative blood loss in the THE Group was signif icantly less than that in the TTE Group ( t = -3 100, P =0 003).ConclusionsVideo- assisted THE is a safe procedure. With the increase of the experience, the incid ence of post- operative complications is subject to further decrease.

Objective To detect the expression of CD70 in peripheral T lymphocytes of patients with systemic lupus erythematosus (SLE) and the effect of azacitidine, an inhibitor of DNA methylation, on it. Methods Blood samples were obtained from 10 patients with active SLE (SLEDAI score ≥5), 10 patients with nonactive SLE (SLEDAI score < 5) and 10 normal human controls. Peripheral T lymphocytes were isolated and cultured for 72 hours. A part of the T lymphocytes from normal controls, which were cultured in the presence of azacitidine at 1 mol/L, served as the methylation-inhibited group. Semiquantitative reverse transcription PCR and flow cytometry were applied to detect the mRNA expression of CD70 and frequency of CD70+CD4+ cells in the cultured lymphocytes, respectively. Results The frequency of CD70+CD4+ lymphocytes was 14.55%±5.49% in normal control group, 85.25%±14.08% in active SLE group, 77.65% ±18.77% in nonactive SLE group, and 81.54%±8.71% in methylation-inhibited group. Compared with the normal control group, a significant increase was observed in both the frequency of CD70+CD4+ lymphocytes (all P < 0.01) and the expression of CD70 expression (all P < 0.05) in other three groups. There was a positive correlation between the frequency of peripheral CD70+CD4+ lymphocytes and disease activity of SLE in patients (r = 0.72, P < 0.05). Conclusions The elevated expression of CD70 appears to play a significant role in the immunologic disarrangement in SLE, and may act as a indicator of disease activity of this disease.

Objective To study the effects of permissive hypercapnia (PHC)on cardiovascular system, oxygen metabolism and gastrointestinal function with acute respiratory distress syndrome(ARDS). Methods In 18 sheep with LPS induced ARDS, hemodynamics, oxygen metabolism and gastrointestinal intramucosal pH(pHi)were observed at different tidal volume(V T).Results PHC was induced by decreasing V T from 16 ml/kg to 4 7ml/kg.Compared with those at V T 16 ml/kg, cardiac index decreased significantly in PHC (P

Five non-anesthetized dogs were intravenously infused with 4% pyrithione at a constant rate of 2 ml per minute for 5 min.During the course of treatment,experimental animals had salivation, nausea, vomiting, urination and defecation. In according with the accumulation of infused drug,the symptoms got worse and worse progressively. Finally, convulsion and respiratory failure appeared and death occured due to heart arrest. One hour after infusion and just before death, the concentration of pyrithione in the blood was 341? 180?g/ml and 657?158?g/ml respectively.The average accumulative lethal dose of pyrithione by intravenous infusion was 0.77?0.07 g/kg for dogs.

A cDNA library prepared from human liver was screened for Factor Ⅶ,a clotting factor in the middle phase of blood coagulation,with P114.12 probe.3 recombinant phages were distinguished,and one of the 3recom-binants was further analyzed and extended by 450 bp as compared with the p114.12 probe.The clone 1 (FⅧhc1) was sequenced and homology between 210 bp of FⅧhc1 and 5559-5769 in FⅧ cDNA was found.It was shown that the FⅧhc1 extended forwardly 800~900bp from 17 exon of FⅧ cDNA.

Alkaline Phosphatase ; blood ; Biomarkers ; blood ; Child, Preschool ; Female ; Humans ; Infant ; Isoenzymes ; blood ; Phosphorus Metabolism Disorders ; blood ; diagnosis ; Risk Factors

Objective To evaluate the effects of recombinant human bone morphogenetic-2-polylactide sustained release nanospheres (rhBMP-2-PLA-Ns) on cultured rabbit osteoblasts in vitro. Methods Rabbit osteoblasts were cultured in vitro, and rhBMP-2-PLA-Ns was added into the medium of the third generation of rabbit osteoblasts. The expression of the proliferating cell nuclear antigen (PCNA) was examined by immunofluoreacence staining, and the formation of tuberculums observed with alizarin red staining. Western blot was used to detect the effects of rhBMP-2-PLA-Ns on the expression of vascular endothelial growth factor (VEGF), which was compared with that in rhBMP-2 group and blank group. Results There was no significant difference in the number of osteoblasts with positive PCNA expression among three groups five days later. Ten days later, the number of osteoblasts with positive PCNA expression in rhBMP-2-PLA-Ns group was significantly higher than that in rhBMP-2 group and blank group, which indicated that rhBMP-2-PLA-Ns could enhance the expression of PCNA. Compared with rhBMP-2 group and blank group, rhBMP-2-PLA-Ns could significantly enhance the formation of tuberculums, with statistical difference (P<0.05). The expression of VEGF was detected in all three groups, and the level of the VEGF expression in rhBMP-2-PLA-Ns group was significantly higher than that in the other 2 groups (P<0.05). Conclusion The biological activity of rhBMP-2-PLA-Ns is superior than that of rhBMP2, and rhBMP-2-PLA-Ns can promote the proliferation, mineralization of osteoblasts and the secretion of VEGF, which has a better prospect in facilitating traumatic bone healing.

Whole mount in situ hybridization with BHC80 RNA probe showed that BHC80 was expressed in zebrafish central nervous system. Morpholino-modified antisense oligonucleotide was injected into zebrafish zygotes to knock down BHC80 expression. BHC80 knockdown resulted in striking decrease of erythrocytes and accumulation of erythrocytes at PBI. Further investigation of embryonic erythrocytes marker βe3 globin and hematopoiesis transcription factors gata1, c-myb and lmo2 by in situ hybridization showed that the erythroid progenitors marked with gata1 in BHC80 knockdown embryos were high proliferation and their differentiation were delayed, which led to decrease of erythrocytes and accumulation of erythrocytes at PBI. Both in situ hybridization and microangiography indicated that vasculature pattern of BHC80 knockdown embryos were almost normal.