Paraffin - embedded tissue of skin biopsy specimens taken retrospectively from 24 patients with cutaneous malignant lymphomas(CML) and 8 patients with cutaneous benign lymphoid infiltrates (CLI) and other dermatoses were estimated with PC10 immunostaining. Our results show a statistical significant difference between PC10 indices for cutaneous genuine histiocytic lymphoma(CGHL), cutaneous germinal center cell-derived lymphomas(CGCCL), cutaneous peripheral T-cell lymphomas(CPTL) other than mycosis fungoides(MF) and Sezary's syndrome (SS),and MF when compared with those for CLI. There is a linear relationship between PC10 index and square root of PC10 density, both of which seem to have a parallel relationship to the seventy of malignancy in CML. The nuclear volume of the positive tumor cell or lymphocyte with PC10 immunostaining may be also useful in differentiating CML from CLI.

OBJECTIVE:To discuss the practice of voluntary principle on the community pharmacy so as to provide some good suggestions for the smooth implementation of the GPP.METHODS:The voluntary principle of GPP was analyzed from several aspects,including the purpose of GPP,the integrated pharmaceutical service level,justice,law and policy and etc.RE_ SULTS:The feasibility of the implementation of voluntary GPP principle in China is expected to be verified,meanwhile,the concerned law and policy should be established in no time and the knowledge of medicine administration should also be publi?cized widely among the public.

Objective To observe the clinical effect of hemabate for controlling atonic postpartum hemorrhage.Methods Form June 2004 to September 2007,21 patients who had postpartum hemorrhage caused by uterine atony,after failing to respond to conventional physical and medical therapies,were conducted an injection of hemabate intrauterinely or intracervically.Results The Postpartum hemorrhage was successfully controlled immediately after administration of hemabate in 19 cases in 15 minutes,the effective rate was 90.5%.And in 30 minutes,there were efficacy in 2 cases,the effective rate was 9.5%.The total effective rate was 100%(P

Objective: To probe into essence of syndrome types of blood stasis syndrome. Methods: High shearing whole blood viscosity (?HB). low shearing whole blood viscosity (?LB), plasma viscosity (?P), aggregation index of erythrocyte (AI), hematocrit (HCT) and levels of serum total cholesterol (CH), triglyceride (TG), plasma fibrinogen (Fb), prostacyclin (PGI2), thromboxane (TXA2) were determined in 216 cases of coronary heart disease with blood stasis syndrome. Results: ?LB and AI increased and PGI2/TXA2 ratio decreased significantly in the group of stagnation of Qi and stasis of blood, showing high aggregation state of blood: CH, HCT, Fb, AI and ?LB increased significantly in the group of blood stasis due to Yin -deficiency, showing high concentration, high agglutination and high aggregation state of blood : ?LB, ?P and TG increased significantly in the group of stagnation of phlegm and blood, showing high viscosity state of blood; only ?HB had some increase in the group of Qi - deficiency and blood stasis . Conclusion: Different syndrome types of blood stasis syndromehave different biochemical material base, and changes of blood physical and chemical properties are closely related with TCM syndrome types, which provides an experimental basis for treatment of subtypes of blood stasis syndrome.

Objective To examine serum levels of IgG, IgA, IgM, IgE and C3 and explore sensitivity of glucocorticoid (prednisone) in primary nephropathyic children to study pathogenesis of primary nephropathy and direct clinical therapy. Methods Examine serum IgG, IgA, IgM and C3 by scattering turbidimetry and IgE by ELISA. Results Compared with healthy children, children suffering from primary nephropathy had lower IgG and higher IgM and IgE, but the same IgA and C3 Simple and nephritic nephropathy had very significant difference in sensitivity of glucocorticoid(x2=18.48 P

Gaucher Disease ; diagnosis ; Humans

Objective:To seek the main incentive factors that influencing medical staff′s enthusiasm through the medical staff satisfaction survey among the grass-roots level medical staff in Shaanxi province.Methods:Choosing the north, middle and south parts of Shaanxi province and using the questionnaire survey, factor analysis method and Krukal-Wallis test were used.Results:Main drive common factor affect the enthusiasm of the staff with train-ing and development, compensation and protection level, management mode and team cooperation, working condi-tions and working environment, and different characteristics of medical staff on incentive satisfaction differences. Conclusion:In order to improve medical staff′s work enthusiasm, it is necessary to combine the Shaanxi regional specific circumstances, to establish a suitable incentive mechanism.

Salteffectinhigh-voltageassistedlaserdesorptionionizationmassspectrometry(HALDI-MS)has been studied under both positive and negative ion modes using L-tyrosine, β-cyclodextrin, α-lipoic acid, as well as the complex formed between α-lipoic acid and β-cyclodextrin as the test analytes. Compared the mass spectra obtained with and without the addition of salt, it is found that HALDI-MS can tolerate a higher amount of salt than electro spray ionization mass spectrometry ( ESI-MS) . Also, the addition of salt can enhance the HALDI-MS signal intensity of the complex ion of α-lipoic acid and β-cyclodextrin, and we suggest that such signal-enhancement effect is due to the formation of the three component complex consisting of α-lipoic acid,β-cyclodextrin and the positive ion of the salt.

Endogenous hydrogen sulfide is the third gaseous signal molecule after nitric oxide and carbon monoxide;it is synthesized through cystathionine-?-synthase and cystathionine-?-cleavage pathway.Hydrogen sulfide can directly react with the KATP channel,reduce extracellular Ca2+ influx,and work together with testosterone,nitric oxide,carbon monoxide,relaxing corpus cavernosum smooth muscle and improving diastolic function of blood vessels.It can also inhibit vascular smooth muscle cell proliferation,promote apoptosis,and improve vascular remodeling.Therefore,in-depth study of H2S in the regulating penile erection can provide a new way for the treatment of erectile dysfunction.

Objective To determine the contents of hypaconitine and mesaconitine in Shubining Granules. Methods The HPLC method was established for the determin ation of hypaconitine and mesaconitine in Shubining Granules. The HPLC was perfo rmed on Akasil C18(4.6 mm?250 mm,5 ?m) column,mobile phase consisted of meth anol-0.1 %triethylamine (70 ∶30),column temperature 30 ℃,and the flow rare was 1.0 mL/min.The detection wavelength was 230 nm.Results A good linearity w as obtained in the range of 0.126～2.520 ?g of Hypaconitine and 0.152～3.04 ?g of Mesaconitine.The average recovery of Hypaconitine was 101.12 %.The average recovery of Mesaconitine was 99.68 %. Conclusion This method is simple and qui ck,and can be used as quality control of Shubining Granules.