Lung nodules are one of the most common pathological changes, thus early detection of lung nodule is very important for the diagnosis medical treatment of lung eancer. In recent years, as the application of multi-slice spiral CT(MSCT), high-resolution CT(HRCT) and low-dose chest CTCLDCT), computer-aided diagnosis (CAD) system will be more essential and more important. Since CAD system can improve the working efficiency of doctors and provide service to more patients, has become the research hotspot and achievement has been made in relevant area internationally recently. This review summarizes the basic methods and applieations of computer-aided detection and diagnosis of lung nodule based on CT image.

ObjectiveTo explore the expression of EZH2 and p53 protein in primary prostate cancer (Pca) and its clinical significance.Methods High-throughput tissue microarray technique and immunohistochemistry was used to detect the expression of EZH2 and p53 protein in 48 human prostate cancer specimens without a history of chemo-radiation therapy and 15 cases of benign prostate hyperplasic (BPH) tissues. The pathological characteristics and the relationship of the expression of EZH2 and p53 protein in primary prostate cancer was analyzed. ResultsImmunohistochemical results showed that the positive rates of EZH2 and p53 protein in prostate cancer were 87.50 ％ (42/48) and 33.33 ％ (16/48), respectively, which were significantly higher than that in BPH tissues[13.33 ％ (2/15) and 0 (0/15)](x2=26.429, x2=5.058,P ＜0.05). The expression of EZH2 and p53 protein was significantly related to Gleason score, TNM stage (P ＜0.05), but not to age and serum prostate-specific antigen (PSA) level (P ＞0.05). The positive expression in patients with Gleason＞6 was higher than that with Gleason≤6(P ＜0.05).The positive expression in patients with T3-T4 stage was higher than that with T1-T2 stage(P ＜0.05).Spearman rank correlation showed a significantly positive correlation between EZH2 and p53 protein (r=0.294, P ＜0.05). ConclusionEZH2 and p53 protein may participate in the pathogenesis of prostate cancer.The overexpression of EZH2 and p53 protein could become an index for the evaluation of the level of malignancy and progression of prostate cancer.Furthermore,combining detection of EZH2 and p53 protein may provide a new theoretical basis for the treatment of prostate cancer.

OBJECTIVE:To provide theoretical basis for the detection of paracetamol by infrared spectroscopy. METHODS:0.002-0.02 g paracetamol was accurately weighed,added into 0.2 g potassium bromide,respectively,fully ground and well mixed, potassium bromide pressing plate method was used for to-be-tested test sample. Using hollow cathode lamp as light,scanning fre-quency was 30 times,resolution was 0.5 cm-1,scan range was 4000-400 cm-1,infrared absorption spectrum was determined and recorded to select the characteristic absorption peaks,then determine the optimized characteristic absorption peaks. Linear and non-linear models were respectively established by using mathematics modeling methods. RESULTS:1 016 cm-1 absorption peak with weaker absorbance but stronger features was selected for the analysis. According to the model establishing and calculation,the accu-racy of the nonlinear model was much higher than the linear model,r=0.942. CONCLUSIONS:Nonlinear quantitative model for quantitatively determining the content of paracetamol is feasible,and suitable for the on-destructive and rapid on-line quality control of paracetamol.

Recent scientific achievements in cell and developmental biology have provided unprecedented opportunities for advances in biomedical research. The demonstration that fully differentiated cells can reverse their gene expression profile to that of pluripotent cells, and the successful derivation and culture of human embryonic stem cells (ESCs) have fuelled hopes for applications in regenerative medicine. Ethical issues concerning the use of cloned human embryos for the derivation of stem cells have stimulated the search for alternative methods for reversing differentiated cells into pluripotent cells. The present state of these reprogramming technologies will be reviewed and their relative success will be discussed.

Stat3 is a member of the STAT (signal transducer and activator of transcription) protein family. It can be activated by many cytokines, growth factors and carcinogens. Activation of Stat3 in the cytoplasm leads to its dimerization, translocation into the nucleus, DNA binding and gene transcription. Constitutively activated Stat3 is associated with cellular transformation, invasion, metastasis, angiogenesis, chemoresistance and radioresistance, and therefore suppress apoptosis of cancer.

BACKGROUND: Intestinal mucosa was damaged by ischemia and hypoxia during severe burn, and injury and infection of oxygen-derived free radicals during reperfusion.OBJECTIVE: To study on the basis of sub-cell level whether danshen can improve respiratory function of mitochondrion of epithelial cell in intestinal mucosa and decrease the production of oxygen-derived free radicals so as to protect intestinal mucosa of burnt rats.DESIGN: Randomized controlled animal study with single sample.SETTING: Department of Burn, the Third Hospital of Chinese PLA.MATERIALS: The experiment was conducted at the Surgery Institute of the Third Hospital of Chinese PLA from December 2001 to February 2002.Totally 96 SD rats of clean grade were randomly divided into normal control group (n=24), burning model group (n=36) and danshen treatment group (n=36). 1 mL danshen parenteral solution had 1.5 g raw materials.METHODS: ① Rats in the burning model group and danshen treatment group were used to establish grade Ⅲ burning models with 20% scald of total body surface. ② Rats in the normal control group were not injured.③ After modeling, 1 mL/kg danshen parenteral solution was slowing injected into rats in the danshen treatment group through femoral vein, but saline was injected slowly into rats in the normal control group and burning model group. ④ Twelve rats from each of the burning mgdel group and the danshen treatment group were sacrificed at 1, 2 and 6 hours after modeling, and 8 rats in the normal control group were sacrificed at relevant time points respectively. ⑤ Samples of small intestine were collected to measure cytochrome aa3, cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa.MAIN OUTCOME MEASURES: Cytochrome aa3, cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa 1, 2 and 6 hours after modeling.RESULTS: Totally 96 SD rats entered the final analysis. ① Level of cytochrome aa3 of mitochondrion of epithelial cells in intestinal mucosa at various time points after modeling: Levels of cytochrome aa3 did not changed obviously in the burning model group and danshen treatment group 2 hours after modeling as compared with that in the normal control group (P＞ 0.05). Six hours after burning, levels in the burning model group were obviously lower than those in the normal control group (P ＜ 0.05), but those in the danshen treatment group were obviously higher than those in the burning model group [(3.16±0.13), (2.5640.15) μkat/g, P ＜ 0.05]. ②Measurements of cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa at various time points after modeling: As compared with those in the normal control group, measurements were decreased obviously in the burning model group 1, 2 and 6 hours after modeling (P ＜ 0.05 or 0.01), but those in the danshen treatment group were obviously higher than those in the burning model group (P ＜ 0.05 or 0.01).CONCLUSION: Danshen can raise the levels of cytochrome aa3, cytochrome C, energy production and SOD, and also reduce the production of oxygen-derived free radicals so as to improve respiratory function of mitochondria of epithelial cells in intestinal mucosa of rats.

Objective To study the role of bacterial collagenase in wound healing with observing capillary neovascularization, epidermis regeneration and collagen fiber reparation in vivo. Method A total of 28 New Zealand rabbits were made into models of superficial soft tissue trauma and divided into two groups. In the experimental group, the right limbs of rabbits were traumatized intentionally in the same length and depth, and their wounds were treated with bacterial collagenase liquor 133 MIU/mL, and the wounds were sutured. Similarly, in the control group, the left limbs of the rabbits were modeled in the same way and their wounds were managed with physiological saline instead and closed. Their wounds were observed continually 1, 2, 3, 5, 7, 10 and 14 days after traumatized. Data were analyzed statistically by using One-way ANOVA, LSD-test, and Wilcoxon rank test. Results There were no significant differences in capillary neovascularization and epidermis regeneration between two groups ( F = 0.12, P = 0.740 and F = 0.95, P = 0.33, respectively), but significant difference in collagen fiber reparation was found between two groups (F = 6.63, P = 0.01). Conclusions In the progress of wound healing, bacterial collagenase can improve the collagen fiber reparation without effects on the new capillary vasculogenesis and epidermis regeneration. The bacterial collagenase does not play a positive role in wound healing.

Objective To discuss dyadic fallopian tube of supermini underbelly cut windowing transplanting tiny simple skill wound method.Methods Choose 25 example mesenchymes headquarters or the gorge headquarter blocks nature barrenness(8 examples)and mesenchyme headquarter or gorge headquarter ectopic pregnancy(17 examples),adopt supermini underbelly 2.5 ~ 3.5 cm cuts to do dyadic fallopian tube of windowing transplant a skill,and transplant skill 15 examples action with the tradition fallopian tube contrasting.Results 25 example patients transplant 29 strip fallopian tube together,operation time shares 68.3 ?22.9 minutes,operation bleeding very few 35.8 +/-28.6 average mL,the skill queen is in hospital time 5.4 +/-0.8 days,no complication happens.Skill last 25 routine fallopian tube exchange the liquid experiment,17(68.0%)examples smooths,7(28.0%)examples exchanges but no free,1(4.0%)regular clausura.Conclusion That the supermini cut of underbelly and the dyadic fallopian tube of windowing transplanting skill is simple and microinvasive,which makes it worth extensive appeication.

Marginal zone lymphomas include three subgroups of lymphoma,with differences on etiology,pathogenesis,molecular cytogenetics,prognosis,as well as involved sites.Based on the reports from the latest ASH annual meeting,the present article introduces the uptodate understanding on the three subtypes of MZL.The recent advances on etioloty,pathogenesis,diagnosis and differential diagnosis were reviewed.

Objective To understand the clinical significance of serum immunoglobulins and C3 at the initial episode on the treat-ment and prognosis of childhood primary nephrotic syndrome(PNS) .Methods 426 children patients with first episode of PNS ad-mitted to the nephrology department of our hospital from January 1 ,2003 to December 30 ,2012 were retrospectively analyzed .The clinical data were collected for conducting the analysis on the immuneglobulins and C 3 levels in different age groups ,clinical classifi-cation ,hormone response ,recurrence ,prognosis and correlation among various pathological types .Results (1)Compared with the healthy children ,the peripheral blood IgG level in childhood PNS was significantly decreased ,while the IgM and IgE level were sig-nificantly increased .(2)The IgE level in steroid-sensitive nephrotic syndrome(SSNS) was higher than that in steroid-resistant ne-phrotic syndrome(SRNS);which in frequent recurrence nephrotic syndrome was higher than that in non-recurrence nephrotic syn-drome .(3) The C3 level in the PNS children patients aged over 1 years and nephritis nephrotic syndrome(NNS) was lower than that in simple nephrotic syndrome(SNS) .Conclusion PNS is correlated with the immune dysfunction .Serum IgE level increase clinically manifests by the steroid sensitivity and frequent relapse ;the lower the C3 level ,the poorer the prognosis .