BACKGROUND:Previous studies have shown that minichromosome maintenance 3 is related with fluorosis, but the expression of minichromosome maintenance 3 in fluorosis patients is not clear yet.
OBJECTIVE:To analyze the mRNA expression level of minichromosome maintenance 3 in peripheral blood from patients exposed to fluoride and normal controls.
METHODS:Eleven patients with mild fluorosis by drinking water (exposure group) and 11 cases of control (non-exposure group) were selected for research. SYBRGreen1 real-time quantitative PCR was used to determine the mRNA expression of minichromosome maintenance 3 in peripheral blood mononuclear cel s, and the liver and renal function indicators were detected.
RESULTS AND CONCLUSION:mRNA expressions of minichromosome maintenance 3 in the exposure group and non-exposure group were (0.573 60±0.102 59) and (0.550 0±0.171 81), respectively, and there was no significant difference between two groups (P>0.05). There were no significant differences in the liver and renal function indicators between two groups. The results indicate that mild fluorosis has no significant effect on mRNA expression of minichromosome maintenance 3 in the peripheral blood mononuclear cel s. More indicators are needed to compressively analyze the effect of fluoride on the liver and renal functions.

OBJECTIVETo evaluate the potential role of BME-10X collagen/hydroxyapatite (HA) bone graft in periodontal tissue regeneration.

METHODSFour 18 months old male beagles in the experiment were treated with non-surgical periodontal therapy. At the sites of mandibular 3rd and 4th premolars at the time of dogs were treated with non-surgical periodontal therapy one week later, the teeth with the same name in the same jaw were selected to the experimental group (T group) or the control group (C group) at random. The defects in T group were filled with BME-10X collagen/HA bone graft while the defects in C group were filled with nothing. The dogs were sacrificed in twelve weeks and analyzed by histopathology.

RESULTSThe defects in T group got more tissue regeneration compared with the defects in C group. The height of new bone (NB) was (3.01 +/- 0.14) mm in T group versus (1.32 +/- 0.11) mm in C group (P < 0.05). The height of new periodontal ligament (NP) was (3.12 +/- 0.19) mm in T group versus (1.35 +/- 0.12) mm in C group (P < 0.05). The height of new cementum (NC) was (3.30 +/- 0.15) mm in T group versus (2.70 +/- 0.12) mm in C group (P > 0.05). The new tissue guided by the bone graft was the same as the normal tissue in histopathology analysis.

CONCLUSIONThe results of the study suggest that BME-10X collagen/HA bone graft is a good bone graft for periodontal tissue regeneration.

Alveolar Bone Loss ; Animals ; Bicuspid ; Bone Regeneration ; Bone Transplantation ; Collagen ; Dental Cementum ; Dogs ; Guided Tissue Regeneration, Periodontal ; Hydroxyapatites ; Male ; Mandible ; Periodontal Ligament ; Regeneration