Objective To elucidate the antitumor activity of exopolysaccharide from Aphanothece halophytica(EPAH).Methods The in vivo inhibition of EPAH on growth of tumor was performed by inoculation of S_(180) sarcoma cells into ICR mice.While in vitro activity against tumor cells was assayed by the growth inhibition of cell lines of S_(180) sarcoma,Smcc7721,and HeLa.The effects of EPAH on immune function were evaluated by the influence on the thymus,spleen,and the number of lymphocytes in blood stream,the influence on proliferation of lymphocytes,the killing activity of NK cells,and the production of NO,IL-1?,and TNF-?. Results EPAH inhibited in vivo S_(180) sarcoma growth with the highest inhibi-(tory) rate of 66.79% and 47.93% in the test mice of pretreatment and simultaneous treatment,respectively.EPAH also displayed in vitro activity against the test cell lines with the highest inhibitory rate being more than 60% at a concentration of 100 ?g/mL.EPAH was found to affect the immune function in mice including increasing the weight of thymus,spleen,and the number of lymphocytes in the blood stream,accelerating the proliferation of lymphocytes,enhancing the killing activity of NK cells,and stimulating the production of NO,IL-1?,and TNF? by macrophages.Conclusion EPAH is an effective antitumor(agent.) It inhibites the tumor cells directly and hence the growth of tumor.Its antitumor activity is probably realized by increasing the weight of immune organs and the number of immunocytes as well as lymphocyte proliferation,enhancing the killing activity of NK cells,facilitating the production of NO and related(cytokines) in tumor-bearing mice.

Objective To assess the safety and the curative effect of the combination of minipercutaneous nephrolithotomy (mini-PCNL) and ureteroscopic lithotripsy (URL) in the treatment of nonhydronephrotic staghorn calculi. Methods The clinical data of 53 eases with non-hydronephrotic staghom calculi treated by mini-PCNL combined with URL were retrospectively analyzed. Results Fifty-three cases (64 renal units) were performed first-stsge operation, 9 renal units were stone free in first-stage operation, 33 renal units were stone free in second-stage operation, other 13 renal units were stone free in third-stage operation. A complete stone clearance rate of 85.9%(55/64) was achieved, and after one to three sessions of mini-PCNL and extracorpereal shock wave lithotripsy afterwards that increased to 95.3% (61/64). Blood transfusion was performed in 3 cases, no major complication was noted in the patients. Conclusions The combination of mini-PCNL and URL has more advantages, less invasions, easier recovery and less complications. It provides a new minimally invasive way for non-hydronephrotic staghorn calculi.

Objective To explore emergency treatment methods for acute renal failure caused by negative imaging ureterolith. Methods There were 36 cases of acute renal failure caused by negative imaging ureterolith, which were finally diagnosed by ureteroscope examination. The negative imaging ureterolith were broken by air pressure ballistic curve shock wave,and taken out of ureter by ureteroscope. All cases were put double-J in ureter. Results Thirty-six cases were got success relieves of ureter obstruction in 24 hours. The urine volume of them were increased, symptoms of urinemia were disappeared, BUN and creatinine were normal after operations. Conclusions The treatment and diagnosis methods by ureteroscope for acute renal failure caused by negative imaging ureterolith are quick and safety, which can treat both side ureterolith at the same time and get reliable and safe effect with less trauma. It should be the first choice to treat acute renal failure caused by negative imaging ureterolith.