Objective To look for a better way to assay vascular endothelial growth factor(VEGF) and explore the correlation of serum VEGF levels with platelet and leucocyte count in breast cancer by comparison of peripheral blood plasma, serum and P-APR. Methods Blood samples were obtained from 42 patients with breast cancer and 20 healthy volunteers. Serum, plasma and P-APR samples were analyzed for VEGF using a commercially available sandwich ELISA, and assayed for semiquantitative measurement using microplatereader. At the same time, samples were analyzed by hematology analyzer. Results The level of VEGF in peripheral blood positively correlated to platelet (health control group: r=0.424, P=0.063) and leucocyte (health control group: r=0.443, P=0.050). The difference in VEGF values of P-APR between the localized breast cancer group and the health control group was statistically significant. Conclusion The level of VEGF in peripheral blood positively correlates to platelet and leucocyte. Platelet and leucocyte possibly secrete VEGF in vitro. Compared with serum and plasma, P-APR is the best section assaying VEGF in peripheral blood.

ObjectiveTo investigate the significance of clinical feature in the source localization of epileptogenic zone in children with frontal lobe epilepsy.MethodsThirty children who were diagnosed frontal lobe epilepsy consecutively admitted to the pediatric department of Shengjing hospital from Oct 2010 to Jun 2011 were recruited for retrospective study.All the cases were subject to video-electroencephalogram monitoring.Results Seventy-six episodes with clear origins of the electroencephalogram were monitored in 30 cases,including orbital frontal attack 11 times (14.5％),dorsolateral frontal attack 33 times (43.4％),mesial frontal attack 32 times (42.1％ ).The correlation analysis showed that the head and eye deviation (47 times,61.8％ ),asymmetric tonic seizures (32 times,42.1％ ) and parakinesia seizures (29 times,38.2％ ) prompted the focus was located in the dorsolateral frontal and mesial frontal.But there was no significant difference between the two areas ( x2 =10.58,9.67,11.55,P ＞0.05).The vocalization,manual pedal automatism and emotional seizure prompted the focus was located in the dorsolateral frontal and orbitofrontal;and the emotional seizure was significant differences between the two areas(x2 =38.68,P ＜0.001 ).Emotional seizure was the characteristic of orbitofrontal attack.Conclusion The clinical features have important significance in the judgment of the source localization of epileptogenic zone in children with frontal lobe epilepsy.The emotional seizure could be considered as the characteristic attack of orbitofrontal.

Objective To investigate surgical techniques of minimally invasive management for multiple calculi in the kidney and ureter. Methods Combined use of ureteroscopic lithotripsy(URSL) and minimally invasive percutaneous nephrolithotomy(MPCNL) was carried out in 21 patients with multiple calculi in homolateral kidney and ureter between January and October 2005 in this hospital.Results Percutaneous nephrostomy was required before the stone removal in 2 patients with pyonephrosis.Of the remaining 19 patients,all the ureteral calculi were completely removed on one session.And the renal calculi were removed by single session in 13 patients,by secondary percutaneous nephrolithotomy in 3 patients,and by tertiary nephrolithotomy in 2 patients.The combination treatment failed in 1 patient because of stricture of uretero-vesical orifice and a conversion to open surgery was needed.Conclusions Combination use of ureteroscopic lithotripsy and minimally invasive percutaneous nephrolithotomy is effective for the treatment of multiple calculi in homolateral kidney and ureter,offering advantages of minimal invasion,rapid recovery,and few complications.

A high efficiency phenol-degrading bacterial strain PS1 was isolated from the drainage ditch of chemical laboratory of East China Institute of Technology.PS1 is a coccus,Gram negative and can live on phenol as its sole carbon and energy source.PS1 to identified as a strain of Raoultella sp.by 16S rRNA gene sequence analysis,which can degrade and tolerate more than 3500mg/L phenol.When phenol concentration is 500mg/L and 1000mg/L,PS1 can completely degrade it in 22 h and 32h,respectively.And while it is between 1500mg/L～3000mg/L,all phenol can be degraded by PS1 in 32h～50h.When phenol concentration is 2500mg/L,the phenol-degrading rate is the biggest and can reach to 78.1mg/h.The optimum growth and phenol-degrading conditions were obtained by orthogonal experiment,which are 25℃,pH6.5,glucose concentration 500mg/L and 20℃,pH7.0,glucose concentration 500mg/L,respectively.

Diabetic retinopathy is a major cause of blindness all over the world, and it is one of the most serious and common microvascular complications of diabetes. Breakdown of the endothelial blood - retinal barrier ( BRB ) , as occurs in diabetic retinopathy, result in vasogenic edema and neural tissue damage, causing loss of vision. The inner BRB is created by complex tight juctions of retinal capillary endothelial cells, this barrier prevents the free diffusion of substances between the circulating blood and the neural retinal, the inner BRB efficiently supplies nutrients to the retinal and removes endobiotics and xenobiotics from the retina to maintain a constant milieu in the neural retina. The central mechanism of altered inner BRB function is a change in the permeability characteristics of retinal endothelial cells caused by elevated levels of cytokines, growth factors, advanced glycation end products, inflammation, hyperglycema and loss of pericytes. This article reviews the relationship between diabetes and the ultrastructure changes of BRB.

Background Retninal neovascular diseases caused by hypoxia has become a major blinding disease,which is lack of effective chemical treatment currently,it's important to study the molecuar mechanism of the disease,so as to guide the clinical medication.Objective This study was to explore the effect of 15 (S)-hydroxyeicosate traenoic acid (15-HETE) on the proliferation of hypoxic retinal microvascular endothelial cells (RMVECs) and its probable mechanism.Methods RMVECs were isolated from C57BL/6J mice and incubated and then identified with anti-Ⅷ factor antibody by immunochemistry and immunofluorescence.The cells were divided into the normoxia group and the hypoxia group.The hypoxia cell models were established by treated with 125 μmol/L CoCl2.The cells were cultured with serum-free DMEM containing endothelial cell growth supplement (ECGS)and high glucose for 48 hours,and then different concentrations of 15-HETE (0.0,0.1,1.0,5.0 μmol/L) were added in the medium for 48 hours respectively to subgroup the groups.The proliferation of the cells (absorbance,A) was detected using MTT.The relative expression levels of protein and mRNA of hypoxia inducible factor-1α (HIF-1α),bcl-2 and caspase-3 were assayed by reverse transcription PCR (RT-RCR)and Western blot.Results The cells showed the positive response for anti-Ⅷ factor antibody with the positive rate of (94.38 ±4.25)％.No significant difference was found in the cell proliferation of various groups under the normoxia condition (F =0.283,P =0.837),but under the hypoxia condition,the proliferation values were significantly different among various groups (F =702.582,P＜0.001).The cell proliferation value in the 1.0 μmol/L 15-HETE group and 5.0 μmol/L 15-HETE group was lower than that of the simple hypoxia group respectively(both at P＜0.05).The inhibitory rates in the 0.1,1.0,5.0 μ mol/L 15-HETE groups were (1.09±0.31) ％,(21.09± 3.53) ％ and (49.86 ±4.15) ％,showing a dosedependent manner.No significant difference was seen in the expression levels of bcl-2,caspase-3 and HIF-1α mRNA in various groups under the normoxia conditions.However,compared with the simple normoxia group,the relative expressions of bcl-2 mRNA and HIF-1α mRNA in the cells were increased by 1.53 folds and 1.7 folds in the simple hypoxia group respectively,and caspasse-3 mRNA expression decreased by 70％ (all at P ＜ 0.05).Under the normoxia condition,the expression of bcl-2 and pro-caspase-3 protein in the cells were not significantly different among the various groups (P＞0.05),however,the expressions of bcl-2 and pro-caspase-3 proteins were elevated by 1.6 folds and 1.9 folds in the hypoxia group in compared with the normoxia group (P＜0.05).Compared with the simple hypoxia group,the expressions of bcl-2 and pro-caspase-3 were lowed by 40.4％ and 42.5％ in the 5.0 μmol/L 15-HETE group (P＜0.05).Conclusions 15-HETE inhibits the proliferation of RMVECs and therefore suppresses neovascularization by down-regulating the expressions of HIF-1α and bcl-2 and the activation of caspase-3 in a dose-dependent manner.

Objective To compare the surgical outcomes between laparoscopic and open surgeries in treatment of ectopic oviduct pregnancy, and investigate the clinical value of laparoscopic surgery. Methods Two hundred and forty-six patients with ectopic oviduct pregnancy in our hospital from June 2009 to March 2013 were retrospectively analyzed. 134 cases were treated with laparoscopic operation and 112 with open surgery. Some parameters such as operative time, blood loss, usage of pain-killer, hospital stay were compared between both groups. Results All operations were successful. Laparoscopic surgery was shown to be superior to open operation in the parameters of operative time, blood loss, usage of pain-killer and hospital stay ( < 0.05) . Conclusion Compared with open operation, laparoscopic surgery has the advantages of less trauma and rapid postoperative rehabilitation. It may become the first line treatment for ectopic oviduct pregnancy.

Objective To understand the distribution characteristics and drug resistance of bacteria isolated from blood cul‐ture in Xinjiang area during 2013 .Methods The identification of isolated bacteria were performed by adopting the France VITEK‐Compact and the ABI series bacterial identification instruments .The antimicrobial susceptibility test was carried out by using the minimum inhibitory concentration (MIC) and Kirby‐Bauer (K‐B) methods .Results A total of 3 962 strains of bacteria were isola‐ted from clinical blood culture ,in which Gram‐positive bacteria and Gram‐negative bacteria accounted for 50 .8% and 49 .2% respec‐tively .The most frequent strains were coagulase‐negative staphylococci (31 .7% ) ,Escherichia coli (23 .2% ) ,Staphylococcus aureus (9 .5% ) ,Klebsiella pneumoniae (8 .7% ) ,Acinetobacter baumannii (3 .6% ) ,Enterobacter cloacae (2 .5% ) ,Enterococcus faecium (2 .5% ) ,Pseudomonas aeruginosa (2 .2% ) ,Enterococcus faecalis (2 .0% ) and Streptococcus pneumoniae (1 .1% ) .The detection rate of extended‐spectrum beta‐lactamase (ESBLs) producing Escherichia coli ,Klebsiella pneumoniae and Proteus mirabilis were 69 .8% ,62 .6% and 66 .7% respectively .The detection rates of methicillin‐resistant Staphylococcus aureus(MRSA) and methicillin‐resistant coagulase‐negative Staphylococcus (MRCNS) was 36 .2% and 86 .3% respectively .The pan‐drug resistant (XDR) strains of Acinetobacter baumannii ,Pseudomonas aeruginosa ,Klebsiella pneumonia were 14 strains(9 .9% ) ,1(1 .2% ) ,2 strains(0 .1% ) ,16 strains(0 .6% ) .No strains resistant to vancomycin or linezolid were found in Staphylococcus and Enterococcus faecalis .Conclusion Among blood culture isolated bacteria in Xinjiang area ,the proportion of Gram‐positive bacteria and Gram‐negative bacteria have little difference .The diversity of bacterial species exist .The resistance to commonly used antibiotics is serious .The distribution situ‐ation of blood culture isolated bacteria should be timely understood .The bacterial drug resistance monitoring should be strengthened to control the nosocomial infections ,guide rational drug use in clinic and control the generation and spread of drug resistant bacterial strains .

Depression, a seriously harmful disease to peoples ’ physical and mental health , is prevailing world-wide.But the etiology and pathological mechanism is still not fully uncovered .With the development of social economy and the pace of life speeding up , increasing pressure and vulnerable emotion cause depression incidence increased rapidly .For the further research on depression , study of depression mechanism and antidepressant drugs highly depends on effective ani -mal models .The degree of similarity of animal models to human disease status and the accuracy and reliability of evaluation methods of animal models directly influence the value of the resuts of experiments .In this article we introduce the common-ly used animal models of depression , the evaluation indicators at organism , organ, and molecular levels , and to provide theoretical references for the further studies on depression .

Adult ; Carcinoid Tumor ; complications ; Cystic Adenomatoid Malformation of Lung, Congenital ; complications ; Female ; Humans ; Lung ; pathology ; Middle Aged