Objective To analyze post-traumatic growth of Chinese caregivers of dementia patients and to explore the caregivers′positive and potential energy, in order to formulate more perfect nursing intervention measures. Method Useing phenomenological method of qualitative research in selecting 6 dementia caregivers in-depth interviews to understand possible posttraumatic growth. Result Five major themes were found: changes in individual cognitive, changes in relations with others, promote their children′s education, reflections and concerns on status of pension-related changes in philosophy of life. Conclusions Dementia caregivers also have the positive performance of posttraumatic growth, in addition to the negative experience. Clinical nurses should actively explore the potential and power from the growth perspective. This is conducive to the caregivers with more health caring for life, so as to improve the quality of care, and ultimately improve the quality of life.

AlM: To improve the diagnosis and treatment of diseases associated with noses and eyes.
METHODS: To retrospectively analyze 39 nasosinusitis patients with the symptoms of eyes as the primary presentation during 2006. 3-2013. 6.
RESULTS:Among the 39 cases, 37 recovered, 2 cases with malignant tumor, which were confirmed by orbital CT scan, sinus CT scan and endoscopic examination.
CONCLUSlON: Some nasal disease without nasal symptoms in the early stage, so patients consult doctors for symptoms of eyes. lf oculists know little about these diseases, it may lead to a high misdiagnosis rate with those patients. Oculists should realize it and get more knowledge to afford the patients timely diagnosis and proper treatments. Please for otorhinolaryngologists' consultation if the patients receiving ineffective treatment in Department of Ophthalmology.

Objective To investigate surgical techniques of minimally invasive management for multiple calculi in the kidney and ureter. Methods Combined use of ureteroscopic lithotripsy(URSL) and minimally invasive percutaneous nephrolithotomy(MPCNL) was carried out in 21 patients with multiple calculi in homolateral kidney and ureter between January and October 2005 in this hospital.Results Percutaneous nephrostomy was required before the stone removal in 2 patients with pyonephrosis.Of the remaining 19 patients,all the ureteral calculi were completely removed on one session.And the renal calculi were removed by single session in 13 patients,by secondary percutaneous nephrolithotomy in 3 patients,and by tertiary nephrolithotomy in 2 patients.The combination treatment failed in 1 patient because of stricture of uretero-vesical orifice and a conversion to open surgery was needed.Conclusions Combination use of ureteroscopic lithotripsy and minimally invasive percutaneous nephrolithotomy is effective for the treatment of multiple calculi in homolateral kidney and ureter,offering advantages of minimal invasion,rapid recovery,and few complications.

Objective:To study the effect of subepithelial connective tissue grafting in covering an exposed root surface.Methods:Eight cases with gingival recession on upper and lower anterior teeth and premolars receited connective tissue grafting received connective tissue grafting were presented with 12 months of follow-up.Results:Initial gingival recession averaged 4.3 mm,with a mean probing pocket depth of 2.3 mm.At the 12-month follow-up,a mean of 3.0 mm of root coverage was observed(ie,71% root coverage,range 60% to 80%).Probing pocket depth averaged 1.6 mm,indicating a 3.6 mm gain of clinical attachment(range 2 to 5 mm).Conclusion:The application of connective tissue graft results in predictable root coverage and clinical attachment while maintaining shallow pockets.

Objective:To construct NBS1 microRNA expressing eukaryotic recombinants,and identify biological activity of recombinants in Hela cell after transfection.Methods:According to sequence of NBS1mRNA,the NBS1 pre-microRNA was designed and synthesized,then cloned into the GFP reporter pcDNA6.2-GW/EmGFP-miR vector and transfected into Hela cell line.To detect integrity of inset fragment through colony PCR and sequencing analysis.The biological activity of recombinants through identify interference efficiency of NBS1 microRNA recombinants by way of Real-Time PCR and Western blot were determined.Results:Sequences of inset fragment in four microRNA expressing recombinants were correct.NBS1 mRNA and protein expression of four microRNA recombinants were decrease,which is the lowest in the NBS1mi-2 group.Conclusion:Four NBS1-targeting microRNA expressing recombinants all have biological activity in Hela cell line,and NBS1mi-2 recombinant has the most interference efficiency.The microRNA expressing plasmid which were successfully constructed and lay foundation for the studies on the tumor gene therapy of microrna targeting NBS1.

Complementary Therapies ; Congresses as Topic ; Humans ; Research

Multiple organ dysfunction syndrome MODS (MODS) is the one of the major factors,which causing the death of the severe patients.More and more research proved that there are some relatonship be-tween MODS with the cellular apoptosis and necrosis.In recent years mesenchymal stem cells has been ap-plied to a variety of diseases as seed cells in the field of rehabilitation,and shows the initial effect in treat-ment and research.Through analysis the distribution and division of mesenehymal stem cells in the body,we found it may play an important role in the treatment in the MODS.We reviewed the mechanism of the MSC dealing with the MODS.

Purpose To investigate the expression of Dkk3 and Cyclin D1 protein in human hepatocellular carcinoma (HCC) and clinical significance.Methods Immunohistochemistry was used to detect Dkk-3 and Cyclin D1 protein expression level in 80 cases of hepatocellular carcinoma and corresponding para-cancer tissue.Results The expression of Dkk-3 in hepatocellular carcinoma was significantly lower than those in corresponding para-cancer tissue (P ＜ 0.05) and the expression of Cyclin D1 in hepatocellular carcinoma was significantly higher than those in CoTesponding para-cancer tissue (P ＜ 0.05).The up-regulation of Cyclin D1 and the down-regulation of Dkk-3 proteins were correlated with pathologic differentiation degree (P ＜0.05).There was a significant inverse correlation between Dkk3 and Cyclin D1 expression (P =0.044,rs =-0.226).Conclusion The abnormal expression of Dkk-3 and Cyclin D1 gene in human hepatocellular carcinoma suggest that Dkk-3 and Cyclin D1 gene may play an important role in the development and progression of the cancer.The combination deteetion of the two biomarkers may provide valuable data for diagnosis and prognosis estimation of HCC.

OBJECTIVE To analyze the high risk factors and etiology character of ventilator-associated pneumonia(VAP) and provide the guidance for preventing VAP. METHODS Eighty three cases given mechanic ventilation over 48 hours in ICU of our hospital from Jan to Oct 2005 were observed.Thirty seven cases met the diagnosis criteria of VAP,while 46 cases without VAP were taken as control. RESULTS Airway secretion was cultivated and Gram-negative germ was the majority(58.0%).The first three pathogens were Pseudomonas aeruginosa(32.0%),Acinetobacter,and Staphylococcus epidermidis,22.0% cases had mixed infection with fungi.Except antiacid treatment,the following factors,such as long time for mechanical ventilation,trachea incision,corticosteroids treatment and coma state had significant relationship with the occurrence of VAP. CONCLUSIONS The occurrence of VAP is related with multiple clinical factors.Clinicians should reduce high risk factors to lower the occurrence of VAP.

Objective To study the bone marrow-derived mesenchymal stem cell(MSC) potential as seed cells for treatment of multiple organ dysfunction syndrome(MODS) in rabbit model, lay fundamental for clin-ical utilization of the MSC for treatment of MODS. Methods MODS rabbit model was established by hemor-rhagic shock combined with endotoxin, isolation and characterization of rabbit bone marrow mesenchymal stem cells labeled MSC with GFP by lentivirus transfection, administration of MSC by ear margin vein injec-tion, MSC integration determined by PCR and pathological section, the MSCs effect on MODS rabbits evalu-ated by physical observation. Results Compared with controls, ransplanted MSCs were found in liver, lung, kidney, MODS rabbits were physically improved obviously. Conclusions MSCs are able to integrate into host without any rejection reaction, and capable of ameliorating MODS evidently.