Objective To study the effects of acetylcholine combined with vincristine (VCR) on A549 cells, furthermore to study the influence that is due to affecting the expression of the two proteins bcl-2 and p53. Methods MTT assay was used to analyze growth inhibition effect of acetylcholine alone and combined with IC50VCR on A549 cells.The expression of bcl-2 and p53 was measured by immunohistological chemistry technique(IHC) and Western blot.Results The inhibition rates for A549 cells with 0.1,1 and 10 μmol/L acetylcholine were (0.050±0.032)％,(0.101±0.021)％,(0.169±0.015)％.The inhibition rates for tumor cells with 0.1,1 and 10 μmol/L acetylcholine joint 0.2 μg/ml VCR were (0.529±0.023)％,(0.545±0.011)％,(0.589±0.015)％,the difference was statistically significant compared with the VCR group (q'values were 1.09,1.37,1.83,P＜0.05).Acetylcholine alone exerted inhibitory effect on A549 cells in a concentration dependent manner and significanrtly enhanced its sensitivity to VCR (P＜0.05).acetylcholibne (0.1-10 μmol/L)combined with IC50VCR decrased the expression of bcl-2 and p53 (P＜0.05).Conclusion Acetylcholine alone and combined with VCR can inhibit the growth of A549 cells. Significant synergistic effect between acetylcholine and VCR is found in inducing cell apoptosis by changing the expression of bcl-2 and p53.

Objective To utilize service quality difference model to analyze the gap between expectation of emergency patients and recognition of nursing service, in order to improve the quality of emergency nursing service by making out the management strategy. Methods A total of 114 emergency observed patients were transversely selected by cluster sampling method and investigated by self-designed current status of emergency nursing quality scale which based on service quality difference model in Taiyuan one class A grade three hospital from March to October 2013. We assessed at time of admitted emergency and discharged from emergency, analyzed the gap of nursing service′s source and types between expectation and recognition and reordered. At the same time, we applied multiple factors analysis method to ensure attribute of nursing service requirement for emergency observed patients. Results The average scores of patients to emergency nursing service was 6. 23 including 5 dimensions and 22 items, and the average score of expectation was 6. 72 (P <0. 05); the SQ average score of 5 dimensions sequenced from large to small: reactive mode, guarantee, stylish, reliability and empathetic; the age, annual times of seeking medical advice and ability of self-care all were the influencing factor of nursing service recognition. Conclusions Service quality difference model is one service quality assessment method from patients′ perspective, and we utilize this model to analyze the expectation and real feeling of service object, help administrators to find out the source caused quality issues and suitable measures targeted to minimize gap.