The qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections.Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins,benzofuran glycosides,flavonoids,and meroterpene were identified.The determination of four major constituents including bavachin,isobavachalcone,bavachinin,and bakuchiol was accomplished by HPLC with UV,MS,and electrochemical detection (ECD).These methods were evaluated for a number of validation characteristics (repeatability,LOD,calibration range,and recovery).ECD obtained a high sensitivity for analysis of the four components; MS provided a high selectivity and sensitivity for determination of bavachin,isobavachalcone,and bavachinin in negative-ion mode.After optimization of the methods,separation,identification.and quantification of the four components in Fructus Psoraleae were comprehensively tested by HPLC with UV,MS,and ECD.

Objective To discuss the health education modes in order to promote the effect of health education for TURP(transurethral resection of prostate)patients and their family members.Methods A total of 380 cases after TURP were divided into the observation group and the control group at random with 190 cases in each group.The discharge instruction for the patients and their family members in the observation group was in the form of health education prescription,while the discharge instruction for the control group was in the form of oral instruction.The awareness rate of disease-related knowledge and incidence of complication were compared between the two groups. Results The awareness rate of disease-related knowledge in the observation group was higher,and incidence of complications was lower than that of the control group.Conclusions The application of health education prescription in the discharge instruction for the patients after TURP can guarantee the effectiveness of health education,simultaneously decrease the incurrence of complications and improve the life quality of patients.

Objective:To establish an HPLC method to determine the content of rosmarinic acid in Wuling capsules. Methods:The chromatographic column was Linksil-ODS (250 mm × 4. 6 mm,5 μm) with a gradient elution mode at the flow rate of 0. 8 ml· min-1,and the mobile phase was acetonitrile-water (32∶68) including 0. 5% acetic acid. The detection wavelength was 319 nm, the column temperature was 25 ℃ and the injection volume was 20 μl. Results: The linear range was 2-80 μg·ml-1 and the standard curve showed good linear regression within the range(r=0. 999 8). And the average recovery was 97. 07﹪ (n=6) with the inter-day precision (RSD) of 1. 35 %. Conclusion:The method is accurate and rapid with good reproducibility in the determination of ros-marinic acid in Wuling capsules, which can be used to control the quality of rosmarinic acid in Wuling capsules.

We observed the normal variations of metatarsal and phalangeal bones in 1363 cases of normal children and youngsters (city group and countryside group), described occurring rate, area, ages and sex of 6 types of the variations, contrasted the difference between city group and country side groups, and discussed some characterastics and clinic significances of normal variations.

To evaluate the efficacy of intravenous administration of aminocaproic acid in pre-venting secondary hemorrhage after traumatic hyphemas. Methods: 60 patients with traumatic hyphemaswere random separated into two groups: (1 ) Routine treated group: 31 patients received intramuscular ad-ministration of adrenosin or dicynone and oral administration of steroids t (2) Arninocaproic acid treatedgroup: 29 patients received aminocaproic acid in an intravenous drip. Results: The incidence of secondaryhemorrhage in the amin0caproic acid treated group was much lower than that in the routine treated group(P

OBJECTIVE:To analysis and identify the liposoluble constituents extracted with supercritical CO 2 fluid ex-traction(SFE)from atractylodes macrocephala by gas chromatography-mass spectrometry(GC/MS).METHODS:Volatile oil was extracted from atractylodes macrocephala,to analsis and identify the chemical components in liposoluble constituents with GC/MS,DB-5MS capillary column was the chromatographic column and helium was carrier gas,the flow rate was 2.0ml/min,and the column temperature was raised from 70℃ to 250℃ at an increasing rate of 10℃/min.EI ion sources were adopted in mass spectra.RESULTS:A total of 19 chromatographic peaks were separated and identified,most of which were unsatu-rated fatty acids and esters,accounting for 96.2% of the total peak area.CONCLUSION:Using GC/MS,the chemical com-position of the liposoluble constituents in atractylodes macrocephala can be analyzed,which provides references for the further exploitation and utilization of atractylodes macrocephala.

OBJECTIVE:To establish a HPLC method for the determination of yohimbine in corynanthe yohimbe K.schum.METHODS:The analysis was performed on C18 column with column under room temperature.The mobile phase consisted of methanol-phosphoric acid buffer-triethylamine(30∶ 70∶ 0.5,pH5.0)with a flow rate of 1.0mL? min-1.The wavelength was set at 270nm.RESULTS:There was a good linear relationship when the sample size of yohimbine was over a range of 10～ 1 000? g? mL-1(r=0.999 8),with recovery at 96.5% and precision at 1.23%.CONCLUSION:This method was simple and reliable,and suitable for the determination of content of yohimbine.

Objective:To establish a rapid and sensitive method for the determination of carbendazim in honeysuckle flowers by LC-MS/MS. Methods:The sample was extracted by acetic ether. The mass spectrometer was operated in the positive ionization elec-trospray ( ESI) mode using selection ion monitoring ( SIM) . The transition m/z 192→160 was used to quantify carbendazim. Results:The method had a satisfactory linearity within the range of 50-5 000 ng·ml-1 for carbendazim (r=0. 999 4), the limit of detection (LOD) was 2. 0 ng·ml-1, and the mean recovery was 93. 2%. Conclusion:The method of LC-MS/MS is sensitive, simple and ac-curate,which is proved to be suitable for the determination of carbendazim in honeysuckle flowers.

Objeetive To explore the correlative factors of early progressive motor deficits in cerebral infarction of moderately size in basal ganglia and the relationship between motor deficit progression and the stem lesion of middle cerebral artery.Methods One hundred and fifty patients with single acute infarction located in white matter of basal ganglia region were recruited in this study retrospectively.All patients were performed brain and neck MRI and magnetic resonance angiography (MRA) within 72 h after onset.They were divided into two groups based on the maximal diameter of infarction on diffuse weighing imaging(DWI):moderate size infarction group(75 patients,maximal diameter of infarction from 1.5 cm to 3.0 cm) and lacunar infarction group(75 patients,maximal diameter of infarction lower than 1.5 cm).The scores of dynamic National Institutes of Health Stroke Scale(NIHSS) in 7 d after the admission and multiple Logistic regression analysis were used and the risk factors of the early progressive motor deficits were analyzed.Results The rate of early progressive motor deficits in moderate size infarction group was significant higher than that in lacunar infarction group [32.0 ％ (24/75) vs.8.0 ％ (6f75)] (P ＜ 0.05).The multiple Logistic regression analysis showed that elevated systolic blood pressure on admission was the independent risk factor for early progressive motor deficits (P =0.016).The rate of stem lesion of middle cerebral artery in moderate size infarction group was significantly higher than that in lacunar infarction group [41.3 ％ (31/75) vs.9.3 ％ (7/75)] (P ＜ 0.01).Conclusions Patients with acute single infarction located in white matter of basal ganglia and with the diameter of 1.5-3.0 cm are more prone to early progressive motor deficits and elevated systolic blood pressure on admission is the most significantly independent factor.The pathogenic mechanism may be associated with the stem lesion of middle cerebral artery.