Platelet play an important role in cerebral ischemial nerve injury. Aspirin (ASA) had been used to treat and prevent stroke in clinic. 30 rabbits were randomly divided into A, B and C groups. In group A ASA was given orally at a daily dosage of 15 mg/kg per rabbit for 5 days before cerebral ischemia; group B cerebral ischemia without giving ASA, and group C was normal rabbits as controls. The cerebral ischemial model was produced by occluding bilateral carotid arteries and bleeding from femoral artery. The results indicated that there was an obvious decrease of PAgT and TXA_2 and had no significance changes in free radicals increasing and Ca~(2+) rising from cerebral tissue in group A. The cerebral edema of group A was less severe than group B. It seemed that ASA had a protective effect on the nerve injury of cerebral ischemia. The derangement of ASA, platelet, free radicals and calcium ions interrelation and their significance on the nerve injury should be further studied.

BACKGROUND AND OBJECTIVES: This study aims to explore the effects of a long non-coding RNA, LINC00525, on colorectal cancer (CRC) and its underlying molecular mechanisms. METHODS: The qPCR, MTT, colony formation, Western blotting, Luciferase reporter and biotin pull-down, shRNA knockdown and DNA fragmentation assays were performed in this study. RESULTS: High expressions of LINC00525 were associated with poor prognosis of CRC patients. LINC00525 knockdown decreased stemness properties and increased sensitivities to oxaliplatin. MiR-507 was a direct target of LINC00525 and overexpression of miR-507 significantly decreased abilities of tumorsphere formation and cell growth. Overexpression of miR-507 resulted in a decrease of expression of cancer stem cell markers and the increase of apoptosis rates. MiR-507 regulated the expression of ELK3. In addition, LINC00525 knockdown decreased the expression of ELK3. Restoration of ELK3 expression abrogated the effects of LINC00525 knockdown. LINC00525 could be served as prognostic marker of CRC. CONCLUSIONS: LINC00525 enhanced stemness properties and increased sensitivities of CRC cells to oxaliplatin by targeting miR-507/ELK3 axis.
Apoptosis ; Biotin ; Blotting, Western ; Colorectal Neoplasms ; DNA Fragmentation ; Humans ; Luciferases ; Neoplastic Stem Cells ; Prognosis ; RNA, Long Noncoding ; RNA, Small Interfering

OBJECTIVETo study the risk factors of male infertility.

METHODCase control study including 94 cases and control group with a ratio of 1 to 1.

RESULTSThe risk factors of male infertility were long time heavy smoking habit (OR = 3.45, 95% CI: 1.95 - 6.10), illegal sexual intercourse (OR = 7.29, 95% CI: 2.54 - 20.89), growing vegetable under plastic in higher temperature (OR = 6.73, 95% CI: 1.91 - 23.69), contact with benzene chemicals (OR = 20.53, 95% CI: 4.67 - 90.25) and having Ureaplasma urealyticum (Uu) infection (OR = 5.55, 95% CI: 2.28 - 13.53).

CONCLUSIONMale infertility was resulted from many factors repeatedly acting on men for long time. In order to prevent male infertility, issues as environmental pollution, occupational protection need to be improved while bad working condition and risky behavior should be changed.

Adult ; Benzene ; toxicity ; Case-Control Studies ; China ; epidemiology ; Environmental Pollution ; adverse effects ; Humans ; Infertility, Male ; epidemiology ; etiology ; Male ; Occupational Exposure ; adverse effects ; Risk Factors ; Smoking ; adverse effects ; Ureaplasma Infections ; complications ; Ureaplasma urealyticum

The study of atrial fibrillation (AF) has been known as a hot topic of clinical concern. Body surface potential mapping (BSPM), a noninvasive electrical mapping technology, has been widely used in the study of AF. This study adopted 10 AF patients' preoperative and postoperative BSPM data (each patient's data contained 128 channels), and applied the autocorrelation function method to obtain the activation interval of the BSPM signals. The activation interval results were compared with that of manual counting method and the applicability of the autocorrelation function method was verified. Furthermore, we compared the autocorrelation function method with the commonly used fast Fourier transform (FFT) method. It was found that the autocorrelation function method was more accurate. Finally, to find a simple rule to predict the recurrence of atrial fibrillation, the autocorrelation function method was used to analyze the preoperative BSPM signals of 10 patients with persistent AF. Consequently, we found that if the patient's proportion of channels with dominant frequency larger than 2.5 Hz in the anterior left region is greater than the other three regions (the anterior right region, the posterior left region, and the posterior right region), he or she might have a higher possibility of AF recurrence. This study verified the rationality of the autocorrelation function method for rhythm analysis and concluded a simple rule of AF recurrence prediction based on this method.

Objective To evaluate the quality of life of patients with type 2 diabetes mellitus in Weifang City, Shandong Province and to explore its influencing factors. Methods A multistage stratified random sampling method was used to investigate patients in endocrine outpatient clinics in four medical institutions in Weifang from July to September 2022. The survey included general information, multi-dimensional evaluation of quality of life with the EQ-5D-5L scale, calculation of health utility values, and analysis of influencing factors using Tobit regression models. Results A total of 397 patients with type 2 diabetes were included in the present investigation, with health utility value of 0.82±0.21 points and visual analogue scale (VAS) score of 79.47±12.81 points. Pain or discomfort, anxiety or depression were more prominent in the study population. Age, diabetic complications, BMI, daily need for care, social support, and daily level of glycemic control were factors influencing health-related quality of life in patients with type 2 diabetes. Conclusion In the actual treatment of type 2 diabetes patients, an emphasis should be placed on protecting elderly type 2 diabetic patients, preventing and controlling the occurrence and development of diabetic complications, and improving daily blood glucose control to further improve the health-related quality of life of the population.