Objective To explore the relationship between personality and mental status of inpatients in the department of neurology of general hospital.Methods 150 inpatients in the department of neurology had been surveyed by Eysenck Personality Questionnaire(EPQ) and Symptom Checklist-90(SCL-90) with the domestic norm as control.Results The examining results of the inpatients in the department of neurology were as following.The scores of neurotisim(N) of EPQ were higher than that of the domestic norm(P<0.05);the scores of extra-introversion(E) and psychoticism(P) of EPQ were lower than that of the domestic norm(P<0.05).The scores of 7 factors including total scores,somatisation,depression,anxiety,hostility,panic and psychosis in SCL-90 were higher than that of the domestic norm( P<0.05).The N scores of EPQ was positively correlated with all factors of SCL-90,the E scoers of EPQ was positively correlated with hostility and paranoia factor of SCL-90,the P scores of EPQ was positively correlated with paranoia factor of SCL-90.Conclusion The mental characteristics of inpatient in the department of neurology of general hospital in acute period are that the incidence of somatisation,depression,anxiety,panic and psychosis are higher than normal people,compulsion and paranoia are as well as normal people.The character of neurotisim and introversion may be the personality background of it.

[Objective] To investigate the clinical effect of treatment of lumbar vertebral instability with posterior interbody fusion using autogenous bone and pedicle screw fixation.[Methods]Thirty-seven cases of lumbar instability were treated with posterior spinal canal,nerve root canal decompression,pedicle screw fixation and autogenous bone grafting,and results were evaluated.[Results]Postoperative follow up time was average 25 months(ranged,12-32 months).Excellent fusion was achieved in 29 cases and good fusion was achieved in 8 cases,with good-to-excellent rate of 100%.According to JOA standard efficacy evaluation,postoperative JOA score improved significantly than preoperative one(P

Objective To study the clinical significance of CD4 + CD25+ Foxp3 + regulatory T cell (Treg) and interleukin 10 (IL-10),transforming growth factor-β1 (TGF-β 1) in patients with steroidresistant asthma (SRA).Methods Seventy-nine patients with asthma were divided into SRA group (31cases) and steroid-sensitive (SSA) group (48 cases).Forty-five healthy subjects were selected simultaneously as control group.CD4+ CD25+ Foxp3 +Treg level was detected by flow cytometry and serum IL-10 and TGF-β1 levels were detected by enzyme-linked immunosorbent assay.Results The percentage of CD4 + CD25 +Foxp3 +Treg in CD4+ T cell and its absolute value in SRA group and SSA group were 0.0225 ± 0.0063,(1.09 ± 0.23) × 107/L and 0.0345 ± 0.0094,(1.35 ± 0.14) × 107/L,they were significandy lower than those in control group [0.0537 ± 0.0128,(2.06 ± 0.27) × 107/L],and SRA group was significandy lower than SSA group,there were statistical differences (P ＜ 0.05).The levels of serum TGF-β1 in SRA group and SSA group were significantly lower than those in control group [(138.12 ± 23.26),(176.25 ± 40.37) ng/L vs.(281.22 ±47.15) ng/L],there was statistical difference (P ＜0.05).The levels of serum IL-10 in SRA group was significantly lower than that in control group [(516.43 ± 86.33)ng/L vs.(763.02 ± 90.19) ng/L],there was statistical difference (P ＜ 0.05).There was no statistical difference in the level of serum IL-10 between SSA group and control group (P ＞ 0.05).The levels of serum IL-10 and TGF-β 1 in SRA group were significantly lower than those in SSA group,there were statistical differences (P ＜ 0.05).The levels of serum IL-10 and TGF-β1 had positive correlation with CD4 + CD25 + Foxp3 +Treg in SRA group and SSA group (P ＜ 0.01).Conclusion The interaction among CD4+ CD25+ Foxp3 +Treg,IL-10 and TGF-β1 may play an important role in the SRA occurrence and development,while by increasing peripheral blood CD4 + CD25 + Foxp3 +Treg number and stabilizing its function can increase IL-10 and TGF-β1 expression,which may be an important way to treat SRA.

Objective:To study the vaccine potency of gene-modified tumor cells. Methods:The EL-4 lymphoma was transduced with recombinant retrovirus containing the murine B7-1 gene. The effect of gene transduction on antitumor immunity was investigated.Results:The appearance, growth rate and surface marker of MHCⅠand MHCⅡ molecules of EL-4 cells transduced with B7-1 gene were the same with control cells except for CD80 positive in B7-1 gene transduced cells. B7-1 gene transduced EL-4 cells resulted in remarkable loss of tumorigenicity in syngenetic mice. EL-4/B7-1 cells could induce system protective immunity. Therapeutic vaccine of EL-4/B7-1 cells could retard the growth of established early-stage EL-4/Wt tumor significantly, but not retard the growth of late-stage EL-4/Wt tumor. Irradiated EL-4/B7-1 vaccine showed weak effect against challenged EL-4 cells.Conclusion:B7-1 gene transduced EL-4 cells can induce system protective immunity. It suggested that this vaccine have a potential application value in human cancer treatment.

Purpose: To study the clinical efficacy, toxicity of preoperative chemotherapy and survival time with Gemicitabine-Cisplatin combination in the treatment of stage Ma( N2) NSCLC. Methods: Thirty patients with stage IIIa( N2) NSCLC were included. Gemicitabine was administered on dl, 8 and 15 at a dose of 1000 mg/m" and Cisplatin at a dose of 100 mg/m on d2. The chemotherapy was repeated every 28days. Results: Thirty patients were evaluable for response. The overall response rate was 70%. Surgical'excision rate after preoperative chemotherapy was 93%. Total surgical excision rate was 70%. Median survival time was 15 months, one year survival rate was 67%. The main toxicity was hematological, thrombocytopenia of grade III-IV appeared in 46% course of treatment, but it did not lead to hemorrhage. Conclusions: Preoperative chemotherapy with Gemicitabine-Cisplatin combination is effective and well-tolerated in the treatment of stage IIIIa( N2) NSCLC. So it is worthy to be further studied and popularized.

OBJECTIVE: To establish a method for the simultaneous determination of the contents of salicylic acid and nitrofural in Fu-La pastes.METHODS: A dual-wavelength spectrophotometry was adopted for the determination of the contents of salicylic acid and nitrofural with water used as solvent.The content of salicylic acid was determined with the detective wavelength set at 296.6 nm and the reference wavelength set at 314.4 nm.The content of the nitrofural was determined directly with the detective wavelength set at 375.0 nm.RESULTS: The linear ranges of salicylic acid and nitrofural were 10.77～43.08 ?g?mL-1(r=0.999 0) and 2.58～10.32 ?g?mL-1(r=1.000 0),respectively;and their average recovery rates were 99.55%(RSD=0.47%) and 100.03%(RSD=0.80%),respectively.CONCLUSION: The method is simple,rapid and accurate,and suitable for the quality control of Fu-La pastes.

Objective: To detect the correlation of the expression of c-erbB-2, p53, PCNA and bcl-2 with the biological behavior of breast cancer. Methods: We used immunohistochemistry to detect the expression of c-erbB-2, p53, PCNA and bcl-2 in 56 breast cancer patients. Results: The expression of c-erbB-2 was 41.1% and was negatively correlated with histological classification but was not correlated with age, clinical stage, ER, or PR. The expression of p53 was 48.2% and was positively related with ER and PR but was negatively correlated with clinical stage and pathological type. The expression of bcl-2 was 50.0% and was negatively correlated with histological classification, ER and PR and was positively correlated with clinical stage and pathological type. PCNA was highly expressed in the 56 cases. Conclusion: We should combine the detection of c-erbB-2, p53, PCNA and bcl-2 with the evaluation of clinical stage, histological degree and pathological type in order to improve the prognostic estimation of breast cancer.

Triple negative breast cancer is a special subtype of breast cancer , including basal -like breast cancer and unclassified breast cancer .Since it was defined in 2005 ,the differences from other subtypes in biological characteristics and poor prognosis of TNBC have became priorities and hotspots which are researched by epidemiologists ,pathologists and clinicians .According to the research ,the risk factors of TNBC include race ,obe-sity,age,women′s reproductive process,oral contraceptive use,physical activity,abortion history,family history, socio-economic factors and so on .This review focuses on race ,obesity,age,women′s reproductive process ,oral contraceptive use ,according to the latest research results of risk factors of TNBC ,helping healthy people get a bet-ter understanding of TNBC and preventing the incidence of TNBC ,suggesting clinicians combine the biological characteristics and epidemiological features and further instructing people to take precautions .This paper also pro-vides theoretical basis for researcher on the further investigaticans of the etiology of TNBC .

To improve the status of management in respiratory failure in China,the project of Study on Pathogenesis and Treatment of Respiratory Failure was designed and conducted by three medical centers(Beijing Institute of Respiratory Medicine-Beijing Chaoyang Hospital,Affiliated to Capital Medical University,Zhongshang Hospital-Fudan University,Guangzhou Institute of Respiratory Medicine-First Guangzhou Medical College)for more than ten years.This project was focused on pathogenesis and treatment strategies of respiratory failure and achieved the following important innovations:(1)Pulmonary Infection Control Window(PIC Window)was firstly proposed and used to determine the time switching point of sequential invasive-noninvasive ventilation;(2)The largest sample size of early use of noninvasive positive pressure ventilation(NPPV)for acute exacerbated COPD(AECOPD)on general ward provided the evidence-based data for expanding the indication of NPPV from treating respiratory failure to alleviating respiratory muscle fatigue;(3)Three new types of masks with intellectual property for NPPV were developed;(4)Designing of intrinsic expiratory end positive pressure(PEEPi)lung model with property of expiratory flow limitation confirmed that PEEPi was the most important factor that increased inspiratory difficulty;(5)The systematic measurement was established for diaphragm strength and endurance;(6)Aquaporin 1(AQP1)was firstly proved the key channel of fluid transportation in the lung;(7)A multicenter prospective cohort study provided objective data that depression had causal effect on COPD exacerbation and hospitalization;(8)Two guidelines for NPPV and mechanical ventilation of AECOPD were initiated by this group.This project has been widely used in clinical practice and promoted the research and treatment of respiratory failure in China.