MiR-200 family is a cluster of miRNAs closely linked to EMT. The miR-200 family contains miR-200a, miR-200b, miR-200c, miR-141 and miR-429. Many studies have shown that miR-200 family are key regulators of EMT and determine the epithelial phenotype. They are important to both EMT development and tumor progression. This paper reviews the advancement in the research of miR-200 family and its mechanism.

Objective: To investigate the expression of survivin in non-small cell lung cancer (NSCLC) and analyze its relationship with clinic opathological features and the expression of p53. Methods: The expression of survivin and p53 in 61 cases of NSCLC were detected by immunohistochemistry using monoclonal antibodies against human survivin protein and P53, respectively. Results: The expression of survivin in NSCLC could be detected in cytoplasm and/or in nucleus. The overall immunoreactivity was found in 77.0% (47/61) of tumors. Cytoplasmic, nuclear and both immunoreactivities were present in 26.2% (16/61), 8.2% (5/61) and 42.62%(26/61) respectively. The cytoplasmic and nuclear immunoreactivities in squamous cell carcinoma (57.6%, 19/33) were higher than that in adenocarcinoma (25%, 7/28) and there was significant difference (P

In order to deepen the reform of public hospitals,Beijing Tongren Hospital has carried out the pilot effort of famous experts team service model in out-patient,established a new mode of interior hierarchical medical services in the hospital.Through the adjustment of outpatient service process and the change of service model,issues of difficult medical treatment and ticket scalpers were solved.The reform has achieved the optimal utilization of outpatient resources inside the hospital,has been the beneficial exploration and practice of hospital internal hierarchical diagnosis and treatment,truly reflected the hospital service concept of taking the patient as the center,and has laid a solid foundation for the future widely hierarchical diagnosis and treatment mode.

Objective To explore the diagnosis and treatment of heterotopic pancreas. Methods A total of 21 histologically confirmed cases of heterotopic pancreas were treated in our Department from 1986 to 1998. We retrospectively analyzed the major clinical symptoms, pathogenesis, diagnostic procedure, results of operation and pathological findings. Results Among all the patients, 9 presenting with severe symptoms were surgically treated and the prognosis was fine. Of the 9 patients, 5 had epigastric pain, 2 obstructive and 2 massive digestive hemorrhage. The other 12 asymptomatic cases were followed up for 1.8 to 12 years. It was difficult to diagnose the disease by clinical situation, observation and imaging examination and only pathological examination could provide the final diagnosis. Conclusions Cases of heterotopic pancreas with symptoms should be surgically managed and the results of resection is satisfactory. Long term follow up needs to be conducted for those asymptomatic cases.

Objective To investigate the effect of PDGF-A on cardiac allograft vasculopathy and myocardial fibrosis in rats.Methods By using inbred Wistar rats as donors,and Sprague Dawley (SD)rats as recipients,heterotopic heart transplantation model was established,Four groups,each having 8 animals,were used.In normal heart group,Wistar rat hearts as blank control;In no rejection group,inbred Wistar rats as donors and recipients without immunosuppressive drugs,and grafts were removed on the day 60;In acute rejection group and chronic rejection group,Wistar rats as donors,SD rats as recipients,and the grafts were harvested on the day 5 without immunosuppresslve drugs in acute rejection group;In chronic rejectlon group,the graits were Immunohistochemistry was used for macrophages(CD68 positive cells)and reverse transcriptionpolymerase chain reaction(RT-PCR)assay for expression of PDGF-A mRNA in cardiac allografts.Results Macrophage infiltration was not found in normal heart group and no rejection group.In acute rejection group and chronic rejection group,macrophage infiltration was found around coronary vessels and in myocardial interstitium,especially in myocardial fibrosis area in chronic rejection allografts.The relative content of PDGF-A mRNA in normal heart group,no rejection group,acute rejection group,and chronic rejection group was 0.26±0.06,0.31±0.04,0.88±0.12,0.94±0.11respectively.PDGF-A mRNA was increased in chronic rejection group and acute rejection group significantly as compared with that in normal heart group and no rejection group(P＜0.01).Conclusion Macrophage infiltration and expression of PDGF-A are associated with cardiac allograft vasculopathy and myocardial fibrosis.

Objective To investigate the mechanism of albtrans retinoic acid (atRA)attenuating cardiac allograft vasculopathy and myocardial fibrosis. Methods With inbred Wistar rats as donors and Sprague Dawley (SD) rats as recipients, heterotopic heart transplantation model was rejection group received same doses of cyclosporine A for 60 days. Grafts were removed on the day 60 post-transplant. Paraffin-embedded sections of cardiac allograft were stained with Masson's trichrome and Van Gieson for examination of myocardial fibrosis and vascular stenosis. Immunohistochemistry was performed to observe CD68 positive cell infiltration. Platelet-derived growth factor-A (PDGF-A)mRNA was detected by using reverse transcription-polymerase chain reaction (RT-PCR). Results The index of fibrosis in chronic rejection group and atRA-treated group was 64. 0 ± 11.9 and 34. 7 ±6. 3 respectively with the significant difference (P<0. 01). Chronic rejection all,grafts showed severe vessel disease. The luminal occlusion index of coronary arteries in chronic rejection group was 62. 9 4± 17. 2, and 40. 1± 8. 2 in atRA-treated group with significant difference (P<0. 01). CD68-positive cell count in atRA-treated group and chronic rejection group was 17. 6 4± 4. 2 and 32. 1 ± 9. 3 with significant difference (P<0. 01). The relative expression levels of PDGF-A mRNA in atRA-treated group and chronic rejection group were 0. 46 ± 0. 08 and 0. 94 4±0. 11 respectively with significant difference (P<0. 01). Conclusion AtRA attenuates cardiac all,graft vasculopathy and myocardial fibrosis. The effects might be induced by inhibition of CD68 positive cell infiltration and PDGF-A mRNA expression.

A composite material of decalcified bone matrix (DBM) and bone cement (BC) combined with bovine bone morphogenitic protein (bBMP) was used to repair canine femoral defect caused by microwave induced hyperthermia. The composite material was examined with scan electron microscope and its biomechanical properties were assessed. Then a canine femoral defect was produced by microwave induced hyperthermia, and the composite material was implanted.Then the femurs were examined with X ray, 99m Tc MDP bone scintigraphy, histology and biomechanics at different postoperative periods. It was found that DBM and BC were tightly connected in a multipolar mode with irregular gaps in a diameter of 400?m to 800?m. Callus was formed in the first month and its amount was most abundant in the third month as shown by X ray examination. The new bone was formed in the composite material,and it was confirmed by bone scintigraphy and histology. The border of new bone was connected with the normal bone. So the composite material could merge with the normal bone finally.

Notch signaling pathway is a highly conserved signaling pathway ,and plays an important role in the individual growth and development process in the vertebrate .Many studies have demonstrated Notch 3 re-ceptor expression is aberrant in a variety of tumors .Recent studies show that aberrant Notch 3 mediated signaling pathway is closely related with the progression of diverse types of tumors .Notch3 plays a vital role in apoptosis , proliferation and differentiation of tumor cells .To promote the studies of Notch 3 as a drug development targets ,the molecular mechanisms of abnormal Notch 3 expression and its role on tumorgenesis ,progression and treatment re-sistance are reviewed in this paper .

Targeted cancer therapy is one of the modalities to increase efficiency and decrease adverse effects of chemotherapy,and the selection of drug vector system is of importance for targeted therapy.An ideal adriamycin targeted vector system should have maximal efficacy and minimal adverse effects in inhibiting cancer cells through different mechanisms.This article reviews the recent progress in adriamycin targeted therapy,including the modes of adriamycin administration,the selection of drug vectors,as well as other chemotherapeutic drugs in targeted therapy of liver carcinoma.

The development of equipment techniques of CT is comprehensively reviewed,including detectors, tube and data processing system. The clinical application of CT are analyzed. Several problems are put forward which must be dealt with urgently.