On the basis of summarizing the general situation of current internal and external network construction of hospitals,the paper introduces the main hidden dangers of hospital network security,puts forward internal and external network security management measures,and elaborates the internal and external network security construction principle,network topology structure,and network construction achievements of the First Affiliated Hospital of Guangxi Medical University.

Humans ; Spirituality

Objective To evaluate CT features and diagnostic value of fungus infection of paranasal sinuses. Methods CT findings in 16 patients with fungus infection of paranasal sinuses proved surgically and pathologically were analyzed.Results CT findings of fungus infection of paranasal sinuses included: unilateral involved, maxillary sinus was mainly affected or other paranasal sinuses involved simaltaneously, the soft tissue within paranasal sinuses presented as hyperplasia of nodule and polyp-like, and there were some punctuated and tubercular calcification inside .Sinus cavity enlarged and the bone wall was destroyed in some cases. Conclusion CT manifestations of fungus infection of paranasal sinuses are of characteristics, CT scan is of important value in diagnosing this disease.

With rapid ageing population,the extensive application of mechanical ventilation and development of organ transplantation,infection caused by gram-negative (G-) bacilli,which have become the common pathogens causing nosocomial (especially ICU) infection,has increased and accounted for more than 65% of the hospital acquired infections.Severe infections in intensive care units are usually caused by Pseudomonas aeruginosa,Acinetobacter sp.,Enterobacteriaceae bacteria and Stenotrophomonas maltophilia,presenting challenges to the treatment.In addition,drug-resistant G-bacilli strains have increased year by year,accompanying with appearance of muti-drug resistant and pan-resistant strains of Pseudomonas aeruginosa and Acinetobacter baumannii.Several pathways may lead to drug resistance of G-bacilli,including permeability changes in ectal membrane,reformation of penicillin-binding protein,enhancement in level of pump MexAB-OprM,overproduction of antibiotic modification enzymes such as cephalosporinase,new type of beta-lactamases especially extended-spectrum beta-lactamases (ESBLs),AmpC beta-lactamase and Klebsiella pneumoniae carbapenemase (KPC).Clinical work is facing the challenges caused by drug resistance of G-bacilli,so the isolation and cultivation of pathogenic bacteria,drug sensitivity test in vitro,monitoring of drug resistance and researches on resistant mechanism should be enhanced,and proper antibiotics should be selected for treatment of G-bacilli infection.Polymyxin is recommended,if necessary,for treatment of multi-and pan-resistance G-bacilli infection,meanwhile the adverse effects of polymyxin treatment should be closely monitored.

Virtual Reality is just emerging as an accepted scientific discipline. It has been widely used in medicine. The major applications of virtual reality for medicine are introduced from three areas :virtual humans,the fusion of virtual humans with real humans and virtual telemedicine. The advanced graphics behind medical virtual reality,such as computational demands,hardware and software environments is briefly discussed. This article also gives a summary of existing distributed VR systems.

Objective:To research expression and function of c-myc gene in hyperacute r ejection of xenotransplantation. 　　Method:Through the model of hyperacute rejection of xenotransplantation wit h isolated rat heart perfused with human plasma,we detected c-myc mRNA expressi on and cell localization with in situ hybridization in hyperacute rejection. 　　Result:c-myc mRNA increased expression in the endothelium cell of heart.Th e value of signal is 450.09±409.99.The value of signal of group of control is 1 74.40±51.50(p＜0.05)。 　　Conclusion:From these study findings,it would appear that hyperacute rejecti on is associated with the elevation of c-myc mRNA expression.

MiR-200 family is a cluster of miRNAs closely linked to EMT. The miR-200 family contains miR-200a, miR-200b, miR-200c, miR-141 and miR-429. Many studies have shown that miR-200 family are key regulators of EMT and determine the epithelial phenotype. They are important to both EMT development and tumor progression. This paper reviews the advancement in the research of miR-200 family and its mechanism.

This article summarized literatures in the recent ten years on the mechanisms of Ci-xue therapy from such aspects as hemorheology, vascular biology, blood constituents and blood coagulating system.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Anti-Anxiety Agents ; therapeutic use ; Anxiety Disorders ; drug therapy ; etiology ; therapy ; Combined Modality Therapy ; Female ; Humans ; Male ; Middle Aged ; Stroke ; complications

AIM: To observe the therapeutical effects of resveratrol on non-alcoholic fatty liver disease and its potential mechanism.METHODS: Male C57BL/6J mice were fed with high-fat and high-cholesterol diet to established non alcoholic fatty liver disease model, and were administrated with resveratrol at doses of 80 mg/kg and 160 mg/kg.After 4-week treatment, the blood sample was collected for determination of total cholesterol (TC) and triglyceride (TG).The liver tissues were harvested for measuring the liver lipid content.The histopathological examination were conducted with hematoxylin and eosin staining.The ceramide levels in the liver tissues were detected by HPLC-MS.The microRNA (mi-RNA)-122 levels in the liver tissues were detected by real-time PCR.The protein levels of serine palmitoyltransferase (SPT) were determined by Western blot.The HepG2 cells were cultured and divided into 5 groups: control group, model group (induced by 0.25 mmol/L oleic acid), model+resveratrol group (treated with 5 μmol/L resveratrol), miRNA-122 siRNA group and resveratrol+miRNA-122 siRNA group.Except control group, the cells in other groups were stimulated with oleic acid and incubated with respective drugs simultaneously for 24 h.The levels of TC, TG and ceramide in the cells of each group were measured.The protein levels of SPT in each group were determined by Western blot.RESULTS: In non-alcoholic fatty liver disease mice, resveratrol dose-dependently reduced the serum TC and TG levels, decreased the lipid deposition, the ceramide level and the SPT protein level, and increased the level of miRNA-122 in the liver tissues.In the in vitro study, compared with model group, resveratrol reduced the serum TC and TG levels, decreased the ceramide level, reduced the SPT protein level.Compared with control group, the levels of TC, TG and ceramide, and the protein expression of SPT were increased in miRNA-122 siRNA group.Compared with miRNA-122 siRNA group, no statistical difference of TC, TG, ceramide and protein expression of SPT in resveratrol combined miRNA-122 siRNA group was observed.CONCLUSION: Resveratrol significantly reduces lipid accumulation by reduction of miRNA-122 and ceramide levels, and decrease in SPT protein levels in the liver.