Objective To compare the features of magnetic resonance imaging (MRI) and electroencephalogram (EEG) to differentiate Alzheimer's disease (AD) and vascular dementia (VD). Methods It was analyzed of the MRI and EEG from 39 patients with AD and 56 patients with VD, to compare the proportion of cerebral atrophy, hippocampal atrophy and leukoaraiosis in MRI, and the proportion of the moderate to severe disorder of EEG and the power spectrum. Results The proportion of cerebral atrophy and hippocampal atrophy was more and leukoaraiosis was less in the AD group than those in the VD group. The proportion of the moderate to severe disorder of EEG increased in AD group, and the ratio of (θ+δ)/(α+β) of whole brain was more in the AD group than in the VD group (P<0.05). Conclusion It is more likely to be AD in dementia patients with atrophy without leukoaraiosis and cerebral ischemic lesions, especially for those with severe abnormal EEG.

To assess the expression of peripheral blood CD62P in patients with non-small cell lung cancer and its clinical significance, peripheral blood CD62P content in 75 patients with lung cancer was detected by immune-flow-cytometry. Among them 45 patients with lung cancer were subjected to comparison of the CD62P contents between pre-operation and post-operation, and comparison with those of 30 normal donors. The results showed that peripheral blood CD62P content in patients with lung cancer was significantly higher than that in normal con-trols(P

Objective To study the expressions of MMP-2 and TIMP-2 in pancreatic carcinoma and their relationship with tumor invasion, local metastasis and prognosis of the carcinoma. Methods The expressions of MMP-2 and TIMP-2 were examined in 32 patients with pancreatic carcinomas by S-P immunohistochemical technique and the correlation with pathological tumor parameters were analyzed. Survival analysis was made by using the Kaplan-Meier method. Results The positive rates of MMP-2, TIMP-2 in 32 patients with pancreatic carcinoma were 56.25% and 75.00%, which were significantly higher than those of the controls(P＜0.05). Expressions of MMP-2 and TIMP-2 were independent of sex, age, histological grading and type, but well correlated with the lymph node metastasis and TNM clinical staging(Ⅰ and Ⅲ, Ⅱ and Ⅲ). There was a significant association between MMP-2, TIMP-2 and prognosis in pancreatic carcinoma. Conclusion MMP-2 and TIMP-2 might be useful markers for biological aggressiveness of this malignancy and might contribute to the invasive properties of pancreatic carcinoma, which can be used to evaluate the prognosis of patients.

Objective To test the degree of acceptance and understanding of the entries for patient reported outcomes of stagnation of qi and blood;To further optimize and modify the entries. Methods A small sample pre-survey and group interviews were used to get the feedback information abut entries of patients, then the panel discussed to modify entries according to the data information. Results After a small sample pre-survey and group interviews, 11 items for modification were obtained, including the rejection in physical pain, belching and so on. Through further optimization and modification, the entries for patient reported outcomes of stagnation of qi and blood covered 41 entries in physiological domain, 11 entries in psychological domain, 1 entry in independent domain, and 8 entries in social domain. Conclusion The core part of the entries for patient reported outcomes of stagnation of qi and blood was formed, which comprehensively reflects the connotation of stagnation syndrome of qi and blood.

Objective To explore if grape seed polyphenols (GSP) reverses human breast cancer multidrug resistance. MethodsIn this study,adriamycin-resistant human breast carcinoma cells ( MCF-7/ADR ) and its parental cells (MCF-7) were used to determine the effect of GSP. MTT assay was adopted to evaluate the cytotoxity. Western blot and Northern blot were performed to observe the expression of MDR1 in MCF-7/ADR. Adriamycin accumulation was measured by flow cytometry (FCM). ResultsGSP ( 1.2 mg/L ,2.4 mg/L) inhibited the expression of P-gp to 80.83%( t =5.58,P

Objective To explore the clinical significance of preserving the intercostobrachial nerve (ICBN) in the radical resection of breast cancer. Method All of 140 cases of breast cancer with Ⅰ - Ⅲ a stage operable eligible,were randomly divided into the experimental group with 79 cases (7 cases were rejected, 1 case was lost) that patients received radical resection of breast cancer with preservation of ICBN,and the control group with 61 cases (2 cases were lost) that patients received routine radical resection of breast cancer based on the time of admission. Analyzed postoperative local recurrence and compared to sensory dysfunction of the two groups. Results There was no significant difference between the two groups of patients in the rate of postoperative local recurrence [0-4.2% ( 3/71 ) vs. 0-3.4% (2/59)] ( P ＞ 0.05 ). The local sensory disturbance of the experimental group [2.8% (2/71 )-11.3% (8/71)] was significantly lower than the control group [54.2%(32/59)-71.2%(42/59)], there was significant difference between the two groups (P ＜ 0.01 ). Conclusion Radical resection of breast cancer with preservation of ICBN is an ideal operation in breast cancer.

Objective To evaluate experience for early diagnosis of pulmonary tembolism alter thoracic injury or surgery. Methods The 9 patients after thoracic injury or surgery with highly-suspected pulmonary embolism underwent chest radiograph of X-ray, helical CT pulmonary angiography, electrocardiogram, radionuclide pulmonary ventilation perfusion imaging, D dimer assay and arterial blood gas analysis respectively and the clinic data and results were analyzed. Results All patients were diagnosed and recovered after 8 patients received thrombolytic therapy and another patient received anticoagulant therapy. Conclusion If there were some symptoms such as suddenly severe dyspnea, cyanosis, with/without chest pains and the feeling of fear to death unable to be explained by original disease on those patients alter thoracic injury or surgery, it's very important for pulmonary embolism to be recognized, and early diagnosis and quickly efficient treatment is the key to prevent death. Helical CT pulmonary angiography is a safe, cheap, noninvasive and effective method for the diagnosis of pulmonary embolism with high sensitivity and specificity.

ObjectiveTo investigate the effect of artificial femoral head replacement surgery in the treatment of elderly patients with intertrochanteric fracture.MethodsThere were 120 cases with intertrochanteric fracture according to the different surgical procedures,they were divided into the observation group with 60 cases and the control group of 60 cases.The observation group were taken hemiarthroplasty.The control group were taken dynamic hip fixation.The situations for the two groups of patients after surgery were compared.ResultsThe observation group:the blood loss was (413.6 ± 125.2) ml,operative time was (65.2 ± 9.8 ) min,ambulation time was (5.9 ± 2.3 ) d,length of stay was ( 15.6 ± 2.6 ) d,complication rate was 11.7 ％.The control group:blood loss was (440.5 ± 126.3 ) ml,operative time was (81.2 ± 12.1 ) min,ambulation time was ( 16.4 ± 4.2) d,length of stay was (25.7 ± 3.1 ) d,complication rate was 33.3％.The blood loss,operative time was not different between two groups.The ambulation time,hospital stay,complication rate were significantly different.There were statistical significance ( P ＜ 0.05 ).ConclusionThe hemiarthroplasty was safe,patients with weight-bearing take exercise early,and bed time was short,had less complications,and could achieve satisfied clinical results,it should be widely applied.

Linoleic acid (C18∶2n-6) and ?-linolenic acid (C18∶3n-3) are found widely in fungi, plants and some lower animals. However, they can not be synthesized in mammals due to lack of △12 and ?-3 fatty acid desaturases. To enable endogenous production of essential fatty acids in mammalian cells, here the stable expression of a Caenorhabditis elegans gene FAT-2 encoding △12 fatty acid desaturase in CHO cells was reported. First, the FAT-2 coding sequence was cloned by RT-PCR. To facilitate high level synthesis of heterogeneous protein, the codon usage of the fatty acid desaturase genes was optimized according to the codon preference of mouse by site-directed mutagenesis, 2 synonymous mutations were introduced into FAT-2 gene by overlapping PCR. The codon-modified gene was finally fused to pBudCE4.1 vector (Invitrogen) under the control of CMV promoter. The expression vector pBudCE-FAT2 was linearized with NheⅠ, and then transfected CHO cells, the cells were under Zeocin selection for nine days and then propagated, then the transfected cells were harvested. The genome and total RNA were isolated for PCR and Norhern blot ananlysis. The results revealed that FAT-2 gene has been integrated into the genome of CHO cells and expressed properly. Fatty acids of total cellular lipids were analyzed by gas chromatography. The results indicate that the expression and function of △-12 fatty acid desaturase resulted in accumulation of linoleic acid. The levels of linoleic acid in transgenic cells were 2.4-fold higher than those in wild-type cells. The moderate linoleic acid in CHO cells was derived from cell culture media uptaken by cell membrane. The results demonstrate that a heterogenous desaturase gene can function well in mammalian cells and prove that transgenic approach is an efficient strategy for changing fatty acid composition of mammals.

Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes. Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus (HCV) by cloning the gene of NS5ABP37 protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (α type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we made a sequence analysis by bioinformatics. Results We screened twenty-five proteins binding to NS5ABP37, including Homo sapiens cyclin Ⅰ (CCNI) gene, Homo sapiens matrix metallopeptidase 25 (MMP25) and Homo sapiens talin 1. Conclusion The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of HCV. And the biological function of NS5ABP37 may be associated with glycometabolism, lipid metabolism and apoptosis.