Objective: To compare the difference of Vitamin B in serum in healthy adults and patiens with Crohn's disease.Methods: 24 healthy adults and 20 patients were measured for Vitamin B1,B6,B12 in serum.A HPLC-DAD method was developed to determine vitamin B extracted directly from human serum.Purospher RP-18 column was used for the gradient elution with mobile phase: 0.04 mol phosphate buffer: methanol(60∶40).Results: The results proved to be linear in measurements of VB1,VB6 and VB12 and the correlation coefficients were 0.999 827,0.985 097 and 0.999 994,respectively.The contents of Vitamin B(VB1,VB6,VB12) in Cronhn's patients were significantly lower than in healthy adults.Conclusion: The measurements of Vitamin B in human serum by HPLC-DAD method are clinically helpful to diagnosis and treatment for Crohn's disease.

Objective To investigate the influence of Smac to the chemosensitivity of cyclophosphamide (CTX) and doxorubicin (DOX) in MCF-7 cells.Methods MCF-7 cells were exposed to CTX,DOX and the combination of both.3-(4,5-dimethyl-2-thiazoly)-2,5-diphenyl-2 H-tetrazolium bromide (MTT) assay was used to estimate the cell viability.Apoptosis was measured by acridine orange staining and Ho.33342/PI dou-ble staining.The mRNA and protein expressions of Smac were determined by RT-PCR and Western blotting.The study also analyzed the changes of pro-apoptotic proteins active caspase-3 and active caspase-9.Results CTX,DOX and the combination of both drugs reduced the cell survival rates in a concentration-dependent manner.The cell viability after being treated with 4.0 μg/ml CTX or 0.2 μg/ml DOX or 2.0 μg/ml CTX and 0.1 μg/ml DOX for 48 hours was (52.90 ± 8.78) ％,(53.35 ± 6.29) ％ and (34.19 ± 5.43) ％,respectively.The drug combination developed a stronger inhibitory effect compared to the single drugs (t =9.051,P=0.014;t =9.074,P =0.014).The Smac mRNA and protein levels in 2.0 μg/ml CTX and 0.1 μg/ml DOX group were 7.47 ± 0.82 and 4.13 ± 0.36,which were higher than those in 4.0 μg/ml CTX group (3.27 ± 0.40 and 2.28 ± 0.27;t =-50.120,P =0.000;t =-42.588,P =0.000) and 0.2 μg/ml DOXgroup (3.34±0.62and2.45±0.40;t=-46.233,P=0.000;t=-39.541,P=0.000).Furthermore,pro-apoptotic proteins active caspase-3 and active caspase-9 increased activity was confirmed by Western blotting.Conclusion Smac plays a vital role in enhancing the sensitivity of chemotherapeutic drugs CTX and DOX in MCF-7 cell line.

Objective To obtain a stably inherited Sprague-Dawley rat model of congenital umbilical hernia by in-breeding, and to observe the structure of umbilical hernia and treat it surgically.Methods Congenital umbilical hernia rats were fostered by full-sib mating.The birth number and umbilical hernia quantity were recorded, and the umbilical hernia rate of rats was analyzed.Six female and 6 male rats with congenital umbilical hernia of 6-month aged F2 generation were selected randomly, among which 2 female and 2 male rats were examined anatomically, and the rest rats underwent surgical suture. Results The umbilical hernia rate was increased along with the increasing inbreeding coefficient, and the rats of F12 and F13 generations were all with congenital umbilical hernia.The umbilical hernia rate in female rats was significantly higher than that in male rats based on the total number of rats from F1 to F13 generation (c2 =11.1, P=0.001).Female and male rats had the same structure of umbilical hernia, and all rats recovered 3-4 weeks after surgery without recurrence.Conclusion After 13 consecutive generations of full-sib mating, a rat model of congenital umbilical hernia with stable genetic properties is successfully established.

Objective To evaluate the dosimetric differences of one RapidPlan Model on different Radiotherapy devices.Methods A RapidPlan Model was built based on 30 reoptimization IMRT plans of cervical cancer patients on typeA LA.Dosimetric differences of automatic optimized IMRT plans using this model on 4 different type LAs,named respectivelyA,B,C andD,were compared with 12 test cervical cancer cases.These four LAs were well commissioned in the treatment planning system (TPS).Student t test was applied for statistical analysis on dosimetric differences.Results Dosimetric differences between A vs.B,C and D were observed on Dmean,HI,CI of PTV50 and PTV45,as well as on V50,V40,V30 of rectum and bladder.Significant dosimetric differences were observed between A and D (P<0.05).Conclusions Automatic planning with RapidPlan model may result in dosimetric differences on different Radiotherapy devices.These differences should be aware of with caution in its clinical application.

Objective:To study the distribution and expression of STAT6 and to examine the suggested roles of STAT6 in the pathogenesis of eosinophil infiltration in nasal polyps and to evaluate the role of STAT6 in the pathogenesis of nasal polyps.Method:All selected cases met the enrollment criteria.Thirty samples of nasal polyps were obtained from patients undergoing nasal polypectomy, and 10 samples of inferior turbinate tissues were from patients undergoing nasal septal reconstruction.STAT6 in nasal polyp tissues from 30 nasal polyposis patients and 10 samples of inferior turbinate tissues were detected with immunohistochemistry(SP) method.SPSS13.0 system was used to perform the statistical analysis.Result:The positive expression of STAT6 was significantly higher in epithelium of nasal polyps than that of the control. The number of eosinophils was significantly higher in epithelium of nasal polyps than that of the control. The difference between these two groups was statitically significant(P<0.05). STAT6 positive cell were localized on epithelium, gland cells and on inflammatory cell of nasal polyps. STAT6 expression was positively correlated with the recruitment of eosinophils in nasal polyps.Conclusion:The high expression of STAT6 protein and the suggested roles of STAT6 in the recruitment of eosinophils in nasal polyps may contribute to the initiation and progression of nasal polyps.

(0.05)).There had significant difference((25.47)?(8.27) vs(32.40)?(5.86)) between them at the seventh day(P

Objective To investigate the expression of CD86 on systemic lupus erythematosus (SLE) peripheral B cells and the effect of triptolide (TL) on it.Methods The percentage of CD86 + B cells was detected by flow cytometer when the peripheral blood mononuclear cells (PBMC) was freshly seperated or after cultured with TL in different concentration for 48 hours.Results The percentage of CD86 + B cells of SLE patients was higher than that of normal controls either when the PBMC was freshly separated ( P

A 24-year-old man presented with a 6-year history of pustules,atrophic scars,and alopecia of the scalp.Dermatological examinations showed generalized alopecia and atrophic scarring of the scalp.A few hairs remained at the vertex region with keratotic papules,pustules,or black sears around the hair follicles.There were also residual hairs at the forehead,temples,and occipital region near the hairline.Clusters of hairs were noted in the occipital region,and keratotic papules were observed around the hair follicles.Erythema,scales,and follicular keratotic papules were found in the superciliary arch.The eyebrows and axillary hairs partially shed and were sparse.Follicular keratotic papules were also found on both cheeks,axillae,chest,abdomen,back and limbs.Stomatological examinations revealed a large space between the upper and lower anterior teeth,anteverted upper anterior teeth,congenital absence of (+1) tooth,deep overbite and deep overjet of anterior teeth.He had a fissured tongue which lacked filiform papillae.Pathological examinations of the skin lesionsshowedathickenedspinouslayer andmassiveinfiltrationsof plasmacytes, neutrophils, and multinucleated giant cells around hair follicles.A diagnosis of folliculitis spinulosa decalvans was made based on the clinical manifestations and histopathological findings.

OBJECTIVETo achieve the rapid identification for seedlings of medicinal Chrysanthemum morifolium, the discriminant equation was established and the software for rapid identification was designed.

METHODLeaf structure of medicinal Chrysanthemum of 12 cultivars was analyzed to establish the discriminant equation based on variance analysis and discriminant analysis. On this basis, the identification program and software (based on the python language) were designed.

RESULTThrough the analysis of variance and multiple comparisons for the 11 leaf parameter index data of 12 different cultivars, it was found that that the leaf parameters were significant different from each other and reached significant levels. The discriminant equation and the rapid identification software were set up based on the analysis of various indicators.

CONCLUSIONThe rapid identification system of seedlings of medicinal Chrysanthemum could be achieved through the establishment of discriminant equation combined with computer technology.

Analysis of Variance ; Chrysanthemum ; anatomy & histology ; classification ; Discriminant Analysis ; Plant Leaves ; anatomy & histology ; classification ; Seedlings ; anatomy & histology ; classification

Objective To investigate the quality of nursing care after the application of tracer methodology in patients undergoing chemoradiotherapy with peripherally inserted central catheters (PICC),evaluate the nurses' adherence to the procedural protocol of PICC,and calculate the number of infectious complications related with the placement.Methods We compared the nurses' adherence to the procedural protocol and the number of infectious complications before (April 2014 to June 2015) and after (July 2015 to October 2016) the tracer methodology was applied at the Chemoradiotherapy Center of Ningbo First Hospital in July 2015.Results After the tracer methodology was introduced to the care of patients with PICC,the nurses had better adherence to the procedural protocol,as shown in the rise of quality control score from (94.00± 3.35) to (97.87± 1.42).The incidence of infectious complications dropped significantly (all P＜0.001).Conclusion The clinical application of tracer methodology can improve the quality of nursing for patients with PICC,reduce the incidence of PICC-related infectious complications,and promote patient safety.