Objective To evaluate the clinical effectiveness of minimally invasive external fixation in the treatment of intertrochanteric fractures of the femur in high-risk patients. Methods By using a monolateral external fixator with oblique pin-insertion, we treated 77 consecutive elderly patients (mean age, 71.3) with intertrochanteric fractures of the femur associated with at least one kind of severe systemic diseases from June 1996 to June 2004. Fractures included 70 cases of anterograde intertrochanteric fracture (According to the Evans classification, there were 5 cases of type Ⅰ fracture, 11 cases of type Ⅱ, 34 type ⅢA, 9 type ⅢB, and 11 type Ⅳ.) and 7 cases of retrograde intertrochanteric fracture. A total of 8 patients were combined with multiple injuries. Results A follow-up was made for 5~60 months (mean, 24.3 months). Complete bone union was achieved in all the patients within a period of 8~20 weeks (mean, 12 weeks). Postoperatively, no non-union or coxa vara occurred. According to the Harris hip scores, 49 patients were classified as “excellent” results, 21 as “good”, and 7 as “poor” (all of which were fatal cases), the “excellent-and-good” rate being 91%. Conclusions Minimally invasive external fixation is an alternative to open internal fixation in the management of intertrochanteric fractures in high-risk elderly patients, especially suitable to those with multiple injuries.

[Objective] To observe the effects of regulating Qi and strengthening spleen on the patients of chronic ulcerative colitis.[Methods] 38 patients of chronic ulcerative colitis(UC)were randomized and divided into experiment group(20 patients)and control group(18 patients)according to the selected standard.The patients of experiment group were treated with complex prescription of regulating Qi and strengthening spleen,the patients of control group were treated with granules of Mesalazine.To observe the symptom of accumulated points and enteroscope appearance on two groups.[Results] The difference on the symptom of accumulated points of experiment group exceeded the control group(P0.05).[Conclusion] There is effect of regulating Qi and strengthening spleen on the patients of chronic UC.

[Objective]To evaluate the unreamed femoral nail with spiral blade (UFN-SB) in the treatment of nonpathologic subtrochanteric femoral fractures.[Method]Twenty-seven patients who suffer subtrochanteric femoral fracture.Those fractures were classified with Seinsheimer’s way.Two cases of typeⅠ,9 cases of typeⅡ(5 of typeⅡ_A,3 of typeⅡ_B,1 of typeⅡ_C),12 cases of typeⅢ(7 of typeⅢ_A,5 of typeⅢ_B),3 cases of typeⅣ,1 case of typeⅤ.[Result]All cases were fellowed-up for 9~42 months,average 15 months.The authordidn't find any severe complications.All these patients could exercise early and bearing weight early.The authordidn't observe broken or loosen or quitted of this kind of internal fixation.[Conclusion]The UFN-SB is a better way for the treatment of subtrochanteric fractures of the femur.It can provide a satisfied restoration,a dependable fixation.It does good use to the concrescence of bone.It prevents short,rotation and varus angulation of femoral.The author get very good curative effect,less trauma,good sciatic function by using this kind of way to heal subtrochanteric femoral fracture.Especially in old people who suffer osteoporosis,this kind of interlocking way decreases the quantity lose of the bone,increases the contact area between bone and internal fixation,provides better angulation stability and rotation stability.

Human umbilical cord stromal cells derived from Wharton's jelly bear the potential of stem cells. They share the common surface markers with mesenchymal stem cells derived from bone marrow. They have a relatively higher proliferation rate and self-renewal capacity and can be successfully differentiated into mature neurons, cardiomyocytes, endothelial, adipocytes, chondrocytes and skeletal cells. Researches have shown their promising foregrounds in regenerative therapeutic applications. This article will give a review about the separation and cultivation methods, in vitro differentiation, and in vitro and in vivo transplantation experiments of the aforementioned stromal cells.

Objective To evaluate the accuracy of 64-slice spiral CT(64 SCT)in assessing the mild and severe stenosis of coronary artery.Methods A total of 72 patients suspected to suffer from coronary artery disease underwent orderly both 64 SCT and selective coronary angiography(SCAG).Volume rendering (VR),multi-planar reconstruction(MPR),maximum intensity projection(MIP)and transverse section wasused to analyze the 64 SCT data.The results were compared with those of SCAG to investigate the accuracy of the 64 SCT in assessing the≥50% stenosis of coronary artery.Results In the 720 SCAG segments of 72 patients.684 of them could be evaluated by 64 SCT.The evaluated rate Was 95.0%.Compared with SCAG,the sensitivity,specificity,positive predictive value,negative predictive value and accuracy rate was 67.4%,92.9%,69.5%,92.3% and 88.0% respectively.Conclusion The accuracy of 64 SCT in assessing mild and severe stenosis is relatively high and it can be used for screening patients with suspected coronary artery diseases as a non-invasive method.

Objective To characterize the effects of Sanchi on the alcoholic hepatopathy rats.Methods 70 SD male rats were randomly divided into five groups:normal group(n=10),control group(n=15),high-dose Sanchi group(n=15),low-dose Sanchi group(n=15),and Tiopronin group(n=15).In order to induce alcoholic hepatopathy,animals in the control,high-dose and low dose Sanchi,and Tiopronin groups were orally dosed with HongXing ErGuoTou(56% ethanol;5g/kg),corn oil(2ml/kg),and pyrazole(27.2 mg/kg)every morning for 14 weeks.Rats in high-dose Sanchi,low dose Sanchi,and Tiopronin groups,were treated every afternoon during the 14-week induction of hepatopathy,with 1.2g/kg Sanchi,0.6g/kg,and 100mg/kg Tiopronin,respectively.All rats were sacrificed 12 hours following the last injection.Blood samples were obtained prior to euthanization.Blood-fat and serum lipoidase activity were measured using an automatic blood biochemical analyzer.Blood haluronic acid(HA),laminin(LN)were determined by ELISA.Histopathological changes of hepatic tissues(i.e.steatosiss,inflammation,and fibrosis)were assessed by microscopic examination of HE and Masson staining of the right lobe of livers.Results Compared with rats from the normal group(treated with ddH2O),rats in the control group have significantly higher steatosis,inflammation,liver fibrosis,and increased level of serum cholesterol,HDL-C,LDL-C,haluronic acid,laminin and AST/ALT activity,(P0.05).More importantly,treatment with high-dose,low-dose Sanchi,or Tiopronin resulted in less severe steatosis,inflammation,fibrosis in the liver,and reduced the levels of serum haluronic acid,laminin(P0.05).Conclusions(1)Daily dosing of alcohol,corn oil,and pyrozole for 14 weeks faithfully replicated the alcoholic hepatopathy symptoms observed in the ALD model,such as hepatic steatosis,immune cell infiltration,blood-fat disturbance,increase in serum AST/ALT activity,and liver fibrosis.(2)Sanchi can significantly alleviate the symptoms in the alcoholic hepatopathy rats including steatosis,improve hepatic function and fibrosis.

BACKGROUND:High fat diet (HFD) can induce overweight and obesity,which have been considered to positively affect bone mineral density (BMD) in adults.However,it is unclear how HFD affects the bone development during childhood.OBJECTIVE:To determine the effect of HFD on bone development in young female mice.METHODS:Twelve female CD1 mice were fed with either HFD or normal fat diet (NFD) starting at 4-week of age for 10 weeks.The bone mineral content (BMC),BMD,fat and lean mass were examined in 14-week old mice using dual-energy X-ray absorptiometry,and bone biomechanical properties were also evaluated using three-point bending test.Serum concentration of bone metabolic markers was measured using enzyme immunoassay.Femora were sectioned in the transverse plane and stained with hematoxylin and eosin for observing the adiposity of bone marrow and changes in trabecular bone area.RESULTS AND CONCLUSION:The body weight and fat mass in HFD-treated mice were increased compared with those in NFD-treated mice,respectively.There were no significant differences between HFD-treated and NFD-treated mice in whole body BMD,BMC,bone area and lean mass.However,the spine BMC and bone area in HFD mice were significantly lower than that in NFD mice,while femoral BMD,BMC and bone area in HFD mice were significantly greater than that in NFD mice.But,there was no statistically different in bone biomechanical values between the two groups.Bone metabolic markers were lower in HFD mice than NFD mice,indicating the less active of bone metabolism in HFD mice.It is suggested that HFD can produce deleterious effect on bone during the active growing phase of young mice.Vertebral bone is more sensitive to this negative effect than cortical bone due to the decreased vertebral mineralization.Weight-bearing bone does not response sufficiently to compensate for the excessive weight gaining.

Objective Speckle is the inherent problem exists in B-Mode ultrasound image,and effective speckle noise reduction will improve the image quality and contribute greatly to clinical doctors to diagnose,especially in fine ophthalmic examination with high frequency ultrasound.Methods This paper proposed a new speckle reduction method based on the Laplacian pyramid and multiscale analysis.In the Laplacian pyramid,true clinical features were separated from noise,according to the different bandpass ultrasound image characteristics in each layer,and the advanced eight directions speckle reduction anisotropic diffusion (SRAD)was adapted to suppressed the speckle noise,and the identified noise and the extracted features were selectively emphasized by suitable edge,coherence and contrast enhancement filtering from fine to coarse scales.The performance of the proposed method was compared to the traditional SRAD method and coherence-enhancing diffusion method by measuring the equivalent number of looks (ENL) and Time cost.Results The ENL and Time cost value of the proposed method were higher compared to the SRAD and the cedif method,i.e 1.172 3 vs 1.122 3,0.929 3 and 0.864 0 vs 1.396 0,1.468 3.Conclusions In summary,the proposed method can more effectively remove the speckle noise while preserving the edge and details of the images.

Aim To develop King cobra antivenom from the egg yolks of immunized hens,and study dynamic expression of IgY in egg yolks.Methods chickens(white Leghorn) were immunized with detoxicated King cobra venom by formaldehyde ,Egg yolk antibody (IgY) was isotated by thiophilic interaction chromatography and identified by SDS-PAGE. Activity of IgY was evaluated by enzyme-linked immunoserbent assay(ELISA) and Double immuodiffusion. Protein was measured using folin-lowry method. Results Specific antivenom could be detected in the yolk laid by the hens 9 d after immunization, At the 60th day after primary immunization ,ELISA titers reached 1∶ 100 000,and 97.5 mg IgY?ml -1 yolk was obtained from thiophilic interaction chromatography. IgY was highly specific, No cross reactivity was observed among IgY and agkistrodon acutus venom and vipera venom ;Little cross reactivity was shown with cobra genus venoms.Conclusion King cobra IgY was obtained and purified from the egg yolks of immunized hens,The dynamic expression of IgY was manitered during the course of immunization,Further investigation is needed.

Objective To investigate the neuroprotective effect of panaxynol on primary cultured cortical neuron against oxidative stress. Methods Viability of panaxynol acted on neuron oxidative stress was monitored by MTT assay and FCM method. Scavenging effects of panaxynol on free radicals were observed in vitro. Effects of panaxynol on SOD activity and GSH-Px, and MDA content in primary neuron injured by H_2O_2 were also determined. Results Panaxynol (2—16 ?mol/L) could dose-dependently protect neuron from oxidative stress induced by H_2O_2; 8 ?mol/L of panaxynol could decrease necrosis and apoptosis rate of neuron significantly (P