Objective To explore the effect and mechanism of Gli2 on EMT and invasion in the hepatocellular car-cinoma cell line SMMC-7721.Methods shRNA of Gli2 and Negative control (NC) shRNA were constructed and transfected into SMMC-7721 cells.shRNA-Gli2 group,shRNA-NC group and blank group were set up .Transwell chambers assay , adhesion experiments were used to detect the ability of invasion ,homogeneous and heterogeneous cells intercellular adhesion of each group .Meanwhile, the qRT-PCR, Western blot were used to examine Gli2, E-cadherin ,N-cadherin and vimentin mRNA and protein expression .Results In different hepatocellular carcinoma cell lines and be along with the increasing ability of the invasion in hepatocellular carcinoma cell lines , Gli2 expres-sion was higher .Compared with the shRNA-NC group and blank control group ,the interfered group extensive cells invasion ability was inhibited ( P <0.05 ) , homogeneous cells intercellular adhesion increased and heterogeneous cells intercellular was opposite ( P <0.05 ) , meanwhile , the expression of E-cadherin was declined significantly ( P<0.05 ) ,the expression of N-cadherin , vimentin raised significantly ( P<0.05 ) .Conclusions Silencing Gli2 gene can reverse the EMT of the hepatocellular carcinoma cell line SMMC-7721 and inhibit cell invasion ,its mecha-
nism may be related to the up-regulation of E-cadherin and the dow N-regulation of N-cadherin, vimentin.

Objective To study the clinical efficacy of Helicobacter pylori (Hp) eradication quadruple therapy combined with Bifidobacterium in the treatment of Hp infection in adolescents.Methods A total of 180 outpatient or hospitalization adolescents with Hp infection in our hospital were randomly divided into Hp eradication quadruple therapy combined with Bifidobacterium group (treatment group,90 cases) and Hp eradication quadruple therapy group (control group,90 cases).The control group was given oral administration of pantoprazole,clarithromycin,amoxicillin,and bismuth potassium citrate,at dose of 40 mg,500 mg,1 000 mg,and 220 mg respectively,at 0.5 h after meal,for twice a day.The treatment group was treated with quadruple therapy plus Bifidobacterium triple viable capsule,at a dose of 630 mg,at 4 hours of morning and evening meals.The two groups were both treated for 14 days.Results The eradication rates of the treatment group and control group were 91.11％ and 62.2％,respectively (P ＜ 0.05).The adverse reactions were mild,including nausea,abdominal distension,diarrhea,constipation,bitter mouth,etc.,and their incidences in the treatment group and the control group was 6.67％ and 21.11％,respectively (P ＜ 0.05).Conclusion Hp eradication quadruple therapy combined with Bifidobacterium might improve the Hp eradication rate and reduce the incidence of adverse reactions in the treatment of Helicobacter pylori infection in adolescents.

Objective To study the clinical efficacy of Helicobacter pylori (Hp) eradication quadruple therapy combined with Bifidobacterium in the treatment of Hp infection in adolescents.Methods A total of 180 outpatient or hospitalization adolescents with Hp infection in our hospital were randomly divided into Hp eradication quadruple therapy combined with Bifidobacterium group (treatment group,90 cases) and Hp eradication quadruple therapy group (control group,90 cases).The control group was given oral administration of pantoprazole,clarithromycin,amoxicillin,and bismuth potassium citrate,at dose of 40 mg,500 mg,1 000 mg,and 220 mg respectively,at 0.5 h after meal,for twice a day.The treatment group was treated with quadruple therapy plus Bifidobacterium triple viable capsule,at a dose of 630 mg,at 4 hours of morning and evening meals.The two groups were both treated for 14 days.Results The eradication rates of the treatment group and control group were 91.11％ and 62.2％,respectively (P ＜ 0.05).The adverse reactions were mild,including nausea,abdominal distension,diarrhea,constipation,bitter mouth,etc.,and their incidences in the treatment group and the control group was 6.67％ and 21.11％,respectively (P ＜ 0.05).Conclusion Hp eradication quadruple therapy combined with Bifidobacterium might improve the Hp eradication rate and reduce the incidence of adverse reactions in the treatment of Helicobacter pylori infection in adolescents.