A simple method for culture of human fetal adrenal medullary cells is described. Enzymatically dissociated human fetal adrenal cells were isolaled by centrifugation on Percoll in the buffer. Adrenal medullary cells with 75%-85% purity were obtained by this procedure. The medullary cells were then plated on plastic culture dishes coated with collagen. After 2-7 days medullary Cells began to flatten and extended processes. A further identification of the cells as medullary in origin was confirmed, by specific catecholamine fluorescence technique. Catecholamine content in the medium was also determined by HPLC.
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