White matter damage in premature infant is the main form of brain damage in low birth and very low birth weight children,but its etiology and pathogenesis is not fully clear.Present studies show that Tolllike receptors(TLR) play an important role in the white matter damage mechanism.TLRs are regulators of innate immune responses.The studies have found that TLRs signaling pathway is activated to secrete large amounts of TNF-α,IL-1β,reactive oxygen species (ROS) and reactive nitrogen (RNS),NO,and other inflammatory factors,inducing the immune response.Nerve immune response damages brain white matter,namely the activation of microglia and proliferation of astrocytes in brain.TLRs provide a new research direction for prevention and treatment.Therefore,the research and progress of toll-like receptors in premature brain white matter injury disease are

ObjectiveTo evaluate the effect of naloxone on L-type calcium current (ICa-L) in isolated rat ventricular myocytes.MethodsAdult SD rats of both sexes aged 8 weeks weighing 200-250 g were used in this study.Single cardiac ventricular myocytes were enzymatically isolated from SD rats.ICa-L was measured in ventricular myocytes and recorded using whole cell patch-clamp technique.Different concentrations (20 and 100 μg/ml) of naloxone were added to the cardiomyocytes.The effect of naloxone on ICa-L was evaluated.ResultsThe peak current of ICa-L Was inhibited by naloxone in a concentration-dependent manner.Naloxone had no significant effect on steady-state activation curve.ConclusionNaloxone inhibits the L-type calcium channel of ventricular myocytes and exerts negative effect on ventricular muscle function.

TCM is the treasure of Chinese culture, and the world's cultural treasure as well. 21st century is the age of information, so China has been using information technology to speed up the internationalization of TCM. With overall expanding of system engineering of TCM informatization, wisdom medicine has become a growth industry, which leads to the demand for high-end information technology talents and management talents. Based on the data of informatization talents training in TCM colleges and universities in China, this article made a statistical analysis, and carried out the related discussion on the cultivation model for characteristics and high-end interdisciplinary TCM informatization talents.

Objective A quantitative study of the DNA skeletal muscle cell nuclei during 0～96h postmortem was performed.Method By the histochemical and image analysis.Result The color and the areas of the nuclei stained with the Feulgen staining method became fainter and smaller gradually at 12 hpm. There was a linear relationship between the degradation rate of the nuclear DNA and the length of the PMI.Conclusion The quantitative image analysis of DNA has a wide prospect of application, but the method has been studied further in aspects of the precise and standadization of the method as well as the effects exerted on the results from the study materials.

Objective To evaluate the expression of ?-defensin-2(BD-2) gene and protein with ventilator-associated pneumonia(VAP) in the old and grown rats.Methods Fifty-eight normal healthy Sprague-Dawley rats were divided into the old group(400～460 g,15～18 months,n=29) and grown group(280～320 g,4～6 months,n=29).Each rat received ventilation(VT=12 ml/kg) through tracheal tube for 24h and was challenged intra-tracheally with Pseudomonas aeruginosa(0.2 ml).The mRNA and protein levels of BD-2 were detected by RT-PCR and Western blot analysis respectively. Results Compared with the grown group,the rats had more severe interstitial pulmonary edema in the old group.There was no dominant difference in BD-2 mRNA and protein expression between the grown group and old group within 3 h,but BD-2 expressions in the grown group were significantly higher at 3 h,6 h,12 h,1 d,2 d and 3 d than those in the old group(P

Objective To investigate the changes in semen of chronic prostatitis patients and their impact on the fertile status. Methods The semen of 86 chronic prostatitis patients and 32 fertile volunteers were analyzed,and the main semen parameters of two groups were compared. Results The mean semen volume, sperm motility and normal sperm morphology of the prostitis patients were 2.08 ml,65.43% and 62.14% respectively,significantly lower than those of fertile men( P = 0.003 , P

Objective To study the thyroid volume(TV) of patients with autoimmune thyroid disease (AITD) and the iodine intake and the factors associated with the goiter of patients with AITD.Methods Questionnaires,physical examination,urinary iodine concentration and thyroid B ultrasound were performed in cases of newly diagnosed patients with AITD.Result The TV of patients with AITD did not associated with the urinary iodine concentration (P ＞ 0.05).The TV associated with patients gender and age and goiter of palpation (P ＜ 0.05).Conclusion The U-shape relation did not show between iodine and goiter in patients with AITD.The goiter of patients with AITD did not associated with the iodine intake.

Objective]To investigate the effect of acupuncture-assisted anesthesia on cerebral state index(CSI) under the steady-state anesthesia of sevoflurane. [Methods] Fifty patients scheduled for elective knee arthroscopy operation were randomly divided into two groups with 25 in each. All patients were induced with sevoflurane, the end-tidal concentrations of sevoflurane in two groups were adjusted to 1.0 MAC and maintained for 15 min after tracheal intubation, acupuncture-assisted anesthesia was used on the affected side in group A, Yanglingquan(GB34) and Zusanli(ST36) were selected as the acupoints. The positive and negative electrodes of the electropuncture apparatus(G8650) were respectively connected with Yanglingquan(GB34) and Zusanli(ST36). People reccived transcutaneous electrical acupoint stimulation with condensation-rarefaction wave(2/15 Hz, 8~12mA) untill the end of operation, patiant's body types SBP, SDP, MAP, HR and CSI were recorded at lying still for 5 min(T1), steady state of sevoflurane(T2), acupuncture-assisted anesthesia after 20 and 30 min(T3, T4). [Results] SBP, DBP, MBP, HR and CSI at T3~T4 were significantly lower in group A than those in group B and T2(P<0.05). [Conclusion]Acupuncture-assisted anesthesia can reduce the CSI under the steady-state anesthesia of sevoflurane and lower blood pressure and heart rate at the same time.

Objective To investigate the efficacy of using the extensor carpi ulnaris as the motor tendon in correcting claw hand deformity and improving hand function.Methods A total of 12 patients with ulnar nerve palsy and claw finger deformity were included into this study from October, 2009 to September, 2011.Results All ceses had an average followed-up of 15.5 months, ranging from 12 months to 24 months.According to the Total Active Movement (TAM) score, there were 4 cases receiving excellent results, 6 cases receiving good results and 2 cases receiving fair results.The claw finger deformity was corrected and intrinsic hand function improved significantly.The average increase in grip strength was 41％ at the 12 months post operation, compared with the preoperative data (P ＜ 0.05).The mean DASH score decreased from 53.6 ± 11.4 preoperatively to 20.4 ± 6.8 postoperatively (P ＜ 0.05).Conclusion The technique of using the extensor carpi ulnaris as the motor tendon is effective in correcting claw fingers, increasing the grip strength and improving overall hand function.This surgical procedure is also beneficial for patients accompanied by the median nerve injury.

Objective To evaluate the efficacy, safety, and patient acceptance of a vacuum-assisted, hand-held biopsy device(Mammotome) in minimally traumatic percutaneous removal of multiple breast masses in female patients under real-time ultrasound guidance. Methods Forty-three women with 99 low-risk multiple palpable masses were involved in the study. Forty-one breast lesions with the diameter between 9 and 20mm, and 58 with the diameter between 21 and 33mm, were removed by the aid of a vacuum-assisted, hand-held biopsy device with 11-gauge or 8-gauge probes. The pathological examination and follow-up evaluation were performed after the procedure. Results The imaged lesions were completely removed successfully from 27 patients and 16 patients using the 8-guage probe and 11-gauge probe, respectively. Most of the lesions were proved to be breast fibroadenoma by pathological examination. No evidence of recurrence was found by physical examination and the ultrasound evaluation during the follow-up period. Most patients were satisfied with the incision, postoperative breast appearance, and the procedure results. Conclusions Minimally traumatic percutaneous removal of palpable benign breast masses using the Mammotome system with ultrasound guidance is feasible, safe, and yields high patient satisfaction.