Objective To study the expression and significance of human tissue kallikrein gene 6(KLK6) in cervical cancer tissues.Methods With glyceraldehyde 3-phosphate dehydrogenase (GAPDH)as reference,the expression of KLK6 in 80 cases of cervical cancer tissues (40 cases with metastasis and 40cases without metastasis) and 40 cases of normal cervical tissues was determined by Taqman probe real-time quantitative reverse transcription-polymerase chain reaction,and analyzed the relationship between cervical cancer occurring and KLK6 expression with clinical data and pathological dats.Results The expression of KLK6 in normal cervical tissues[(1.06 ± 0.40) × 10-3] was lower than that in cervical cancer tissues without and with metastasis[(4.41 ± 1.70) × 10-3,(32.22 ± 6.70) × 10-3],and there was significant difference (P＜0.01).The expression of KLK6 in Ⅰ a,Ⅰ b,Ⅱ a stage of cervical cancer tissues with metastasis was (30.42 ± 5.00) × 10-3,(31.64 ± 1.30) × 10-3,(33.02 ± 8.00) × 10-3,and there was no significant difference among them (P ＞ 0.05).The expression of KLK6 in Ⅰ a,Ⅰ b,Ⅱ a stage of cervical cancer tissues without metastasis was (4.12 ± 1.10) × 10-3,(4.35 ± 1.30) × 10-3,(4.82 ± 1.90) × 10-3,and there was no significant difference among them (P＞0.05).There was significant difference in the expreesion of KLK6 in Ⅰ a,Ⅰ b,Ⅱ a stage between cervical cancer tissues with metastasis and cervical cancer tissues without metastasis (P ＜0.01).Conclusion KLK6 can stimulate the cervical cancer cell proliferation,and participate in the progresses of cervical cancer metastasis.

Objective To enhance the anti-tumor efficiency of artemisinin and to explore the anti-tumor and sensitivity-enhancing mechanism of Artemisinin Sensitizing Compound(ASC).Methods The growth-inhibition rate of HepG2 cells was determined by methyl thiazolyl tetrazolium(MTT) method.Tumor-bearing nude mice were given inoculation of HepG2 cells subcutaneously.After treatment for 15 days,the tumor-inhibition rate was calculated according to the tumor mass weight.Results The growth-inhibition rate of tumor cells in ASC group(artesunate combined with diethyl maleate,mercaptosuccinic acid and aminotriazole),was increased 43.84%,and the tumor-inhibition rate was three times as much as that in artesunate alone group with the same titer.The amount of antioxidant agents and the activity of antioxidant enzymes were higher in the control group than those in the artesunate alone group and ASC group,while were lower in ASC group than those in the artesunate alone group.Conclusion ASC has anti-tumor and sensitivity-enhancing effect through decreasing the amount of anti-oxidant agents and inhibiting activities of anti-oxidant enzymes.

Objective To afford a clinical reference for applications of TIP-300 insert earphone in accordance with its difference in inter-aural attenuation from the telephonics TDH-50P supra-aural earphone.Methods Detections of inter-aural attenuation for pure tone air conduction were performed on the enrolled individuals combined with unilateral anakusis and one normal ear (male and female were 13 and 22 respectively) by GSI61 audiometer?TIP-300 insert earphone or telephonics TDH-50Psupra-aural earphone.Results Inter-aural attenuation differences were significant ( P

Brain ischemic tolerance refers to endogenous brain protective mechanism during severe ischemic injury of the body. Ischemia preconditioning is an effective mean to induce brain ischemic tolerance. However, the invasive and ethical limitation made the application of ischemic preconditioning difficult in the clinical practice. Studies found that extracellular signal regulated kinase (ERK) signaling pathway played an important role in the formation of brain ischemic tolerance. Meanwhile, brain ischemic tolerance induced by traditional Chinese medicine (TCM) had its unique advantages. This article reviewed effects of ERK signaling pathway in the inducing of brain ischemic tolerance and TCM intervention in recent years.

Most of the transfusion adult outpatients are old people with chronic disease, and theirsmall blood vessels become less elastic with age. They need long course and repeated localised puncture site. On the base of that fact, the author describes the 5.5gauge scalp acupuncture and 7 gauge scalp acupuncture about their use on the patients. The 5.5 gauge scalp acupuncture is smaller and shorter, so it has the advantage of high success ratio and it is easier to be fixed. Additionally, the transfusion speed is easy to be controlled, which reduces the ratios of transfusion reaction and adverse drug reactions. The pinhole is small and can be healed faster. The congestion around the puncture location is less than that of the 7 scalp acupuncture. It can alleviate the pain for patients and increase satisfaction. To sum up, the 5.5 gauge scalp acupuncture should be used widely as we allocate“people oriented”and“All for the patients”.

0.05) of HKG testing slip had no significant differ-ence in statistics compared with pour plate method.As the gelling agent,HKG shows good performance in bacterial testing slip,and can also improve the detection efficiency.

Objective To observe the effect of Wenxiao Decoction in treating the patients with carotid atherosclerostic (CAS) plaque and assess the safety. Methods Sixty patients with CAS plaque were randomly allocated to treatment group (33 cases) and control group (27 cases). The control group was given routine therapy, and the treatment group was given Wenxiao Decoction additionally. And they were further divided into half-year treatment group and half-year control group, 1-year treatment group and 1-year control group. Carotid artery color Doppler ultrasound and hepatorenal function were examined before and after treatment. Results Compared with the control groups after therapy, the average numbers of plaques, maximal plaque thickness, Crouse plaque integral were decreased both in 1-year and half-year treatment groups (P<0.05, P<0.01). The plaque stability of 1-year treatment group was better than that of half-year treatment group. The average numbers of plaques, plaques size and Crouse plaque integral increased in control groups after routine therapy (P<0.05, P<0.01). Bilateral common carotid artery diameter, intima-medial thickness (IMT) and hepatorenal function had no statistical difference between before and after treatment both in treatment and control groups (P>0.05). Conclusion Wenxiao Decoction is effective and safe in treatment of CAS plaque, and plaque stability is more obvious with the extension of treatment time.

AIM: To investigate whether the circadian mRNA expression of the renin-angiotensin system (RAS) components (ATNG、AT_1R、AT_2R) in myocardium is correlated with left ventricular hypertrophy in both spontaneously hypertensive rats (SHR) and renal hypertensive rats (RHR). METHODS: The model of RHR was established using 2K1C (two-kidney one clip). SHR, RHR, and normotensive control rats (CON) were housed in a separate environment-controlled room for two weeks, in which a strict 12-hour light/12-hour dark cycle regimen was enforced (lights on at 900, zeitgeber time [ZT0]). Rats were killed every 4 hours from 900 am (ZT0) to 500 pm (ZT20) on three separate days. We examined the circadian mRNA expression of ATNG、AT_1R、AT_2R in the hearts of SHR, RHR and CON rats by RT-PCR. RESULTS: Heart weight to body weight ratios and left ventricle weight to heart weight ratios indicated a significant increase in SHR and RHR compared with normotensive rats. The cardiac mRNA expression of the RAS components showed circadian oscillations in SHR, RHR and CON rats. The amplitudes of these circadian fluctuations were greater in the SHR and RHR than in the CON rats. The mRNA levels of the RAS components were increased in the SHR and RHR compared with the CON rats at many time points. CONCLUSION: The data indicate that overexpression and enhanced amplitudes of circadian fluctuations of RAS components in SHR and RHR may play a role in the development of left ventricular hypertrophy.

Objective To explore the methods for training the abitity of critical thinking of ICU nurses.Methods ICU nurses were trained in terms of knowledge training, case study, care rounds, self-reflection, case discussion, simulation training and seminars. The Chinese version of the critical thinking disposition inventory(CTDI-CV)was used before and one year after the training.Result The scores on CTDI-CV as well as the items after training were all significantly higher than that before training(P<0.05).Conclusion The knowledge and ability in critical thinking can be improved by knowledge and skills training.

Objective To investigate the frequency of ocular complication and the quality of patient's life after radiation therapy in nasopharyngeal carcinoma ( NPC ) . Methods 254 NPC patients who initially received radiation were followed and analysed. Visual acuity, automational visual field, slit-lamp microscopic findings, pattern visual evoked potential ( P-VEP ) and fundal findings were determined before, during and after radiation therapy. The severity of retinal impairment was assessed according to the international criteria on late tissue effects. Results The radiation dose was more than 70 Gy in 241 (94.9%)NPC patients, giving a radiation retinopathy incidence of 8. 7 % (22) patients after a mean of 46. 8 ? 14. 4 months. After being diagnosed as radiation retinopathy, 16 patients received combined-modality therapy of the modern medicine and Chinese traditional medicine. The disease condition was controlled in 56% (9) patients but progressed into optic neuropathy in 7 patients, 3 of whom developed radiation encephalopathy in 14 to 20 months after onset of retinopathy. The morbidity of radiation retinopathy was not associated with the patient' s age, but was related to the radiation dose. The retinopathy rate was as high as 13.6% in the 75-79 Gy group, which is significantly higher than 5.6% in the 70-74 Gy group ( P